U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

folD bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945221, updated on 17-Dec-2024

Summary

Go to the top of the page Help
Official Symbol
folD
Official Full Name
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
Primary source
ECOCYC:EG10328
Locus tag
b0529
See related
ASAP:ABE-0001818
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ads; ECK0522
Summary
Early purification studies indicated that the bifunctional protein had a structure of five dissimilar subunits. [More information is available at EcoCyc: EG10328].
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Sequence:
NC_000913.3 (556875..557741, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene PF04307 family inner membrane protein YbcI Neighboring gene putative RNA-binding protein YbcJ Neighboring gene putative fimbrial protein SfmA Neighboring gene putative fimbrial chaperone SfmC

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. A complex of N5,N10-methylenetetrahydrofolate dehydrogenase and N5,N10-methenyltetrahydrofolate cyclohydrolase in Escherichia coli. Purification, subunit structure, and allosteric inhibition by N10-formyltetrahydrofolate. Dev IK, et al. J Biol Chem, 1978 Jun 25. PMID 350870
  2. Physical map location of the Escherichia coli gene encoding the bifunctional enzyme 5,10-methylene-tetrahydrofolate dehydrogenase/5,10-methenyl-tetrahydrofolate cyclohydrolase. Dimri GP, et al. J Bacteriol, 1991 Sep. PMID 1885508, Free PMC Article
  3. Purification and properties of 5,10-methylenetetrahydrofolate dehydrogenase from Escherichia coli. Donaldson KO, et al. J Biol Chem, 1965 Nov. PMID 4378935
  4. Purification, crystallization, and preliminary x-ray studies of a bifunctional 5,10-methenyl/methylene-tetrahydrofolate cyclohydrolase/dehydrogenase from Escherichia coli. Cheung E, et al. Proteins, 1997 Feb. PMID 9061797
  5. The crystal structure of a bacterial, bifunctional 5,10 methylene-tetrahydrofolate dehydrogenase/cyclohydrolase. Shen BW, et al. Protein Sci, 1999 Jun. PMID 10386884, Free PMC Article

See all (19) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. characterization of wild-type and mutant (R191E, G122D, D121A, Q98K, C58Y, K54S, and Y50S) FolD together with three-dimensional modeling has allowed us to develop a better understanding of the mechanism for substrate binding and catalysis by E. coli FolD.
    Title: Impact of Mutating the Key Residues of a Bifunctional 5,10-Methylenetetrahydrofolate Dehydrogenase-Cyclohydrolase from Escherichia coli on Its Activities.
  2. N-terminus verified by Edman degradation on mature peptide
    Title: Purification, characterization, cloning, and amino acid sequence of the bifunctional enzyme 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase from Escherichia coli.
Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables methenyltetrahydrofolate cyclohydrolase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables methenyltetrahydrofolate cyclohydrolase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables methenyltetrahydrofolate cyclohydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables methylenetetrahydrofolate dehydrogenase (NADP+) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables methylenetetrahydrofolate dehydrogenase (NADP+) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables methylenetetrahydrofolate dehydrogenase (NADP+) activity IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in L-histidine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in methionine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within methionine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in one-carbon metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in purine nucleotide biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in tetrahydrofolate interconversion IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in tetrahydrofolate interconversion IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

Go to the top of the page Help
Preferred Names
bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
NP_415062.1

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    556875..557741 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415062.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415062.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    W1X0K2
    Conserved Domains (1) summary
    PRK10792
    Location:1 → 285
    PRK10792; bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.