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uvrB UvrABC excision nuclease subunit B [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945385, updated on 3-Dec-2024

Summary

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Official Symbol
uvrB
Official Full Name
UvrABC excision nuclease subunit B
Primary source
ECOCYC:EG11062
Locus tag
b0779
See related
ASAP:ABE-0002658
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
dar-1; dar-6; ECK0768
Summary
[More information is available at EcoCyc: EG11062].
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Genomic context

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Sequence:
NC_000913.3 (813526..815547)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene biotin synthase Neighboring gene 8-amino-7-oxononanoate synthase Neighboring gene malonyl-acyl carrier protein methyltransferase Neighboring gene dethiobiotin synthetase Neighboring gene putative transferase YbhK Neighboring gene GTP 3',8'-cyclase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Functions of base flipping in E. coli nucleotide excision repair. Malta E, et al. DNA Repair (Amst), 2008 Oct 1. PMID 18638572
  2. Introduction of a tryptophan reporter group into the ATP binding motif of the Escherichia coli UvrB protein for the study of nucleotide binding and conformational dynamics. Hildebrand EL, et al. J Biol Chem, 1998 Apr 3. PMID 9525874
  3. Accessibility of epitopes on UvrB protein in intermediates generated during incision of UV-irradiated DNA by the Escherichia coli Uvr(A)BC endonuclease. Kovalsky OI, et al. J Biol Chem, 1998 Aug 14. PMID 9694852
  4. Detection of DNA damage by Escherichia coli UvrB-binding competition assay is limited by the stability of the UvrB-DNA complex. Routledge MN, et al. Carcinogenesis, 1997 Jul. PMID 9230288
  5. Thermodynamic characterization of the interaction of mutant UvrB protein with damaged DNA. Ma H, et al. Biochemistry, 2004 Apr 13. PMID 15065864, Free PMC Article

See all (103) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Real-time investigation of the roles of ATP hydrolysis by UvrA and UvrB during DNA damage recognition in nucleotide excision repair.
    Title: Real-time investigation of the roles of ATP hydrolysis by UvrA and UvrB during DNA damage recognition in nucleotide excision repair.
  2. Recruitment of UvrBC complexes to UV-induced damage in the absence of UvrA increases bacterial survival.
    Title: Recruitment of UvrBC complexes to UV-induced damage in the absence of UvrA increases cell survival.
  3. ATPase domain, ATP binding domain and/or the DNA binding domain of the UvrB protein are required for DNA repair.
    Title: Evidence for involvement of UvrB in elicitation of 'SIR' phenotype by rpoB87-gyrA87 mutations in lexA3 mutant of Escherichia coli.
  4. study visualized how UvrA and UvrB interacted with DNA individually or together by single- molecule imaging of quantum-dot-labeled proteins
    Title: Collaborative dynamic DNA scanning by nucleotide excision repair proteins investigated by single- molecule imaging of quantum-dot-labeled proteins.
  5. found that the N-terminal domain and the unstructured region at the C-terminus of UvrD interact with UvrB.
    Title: The unstructured C-terminal extension of UvrD interacts with UvrB, but is dispensable for nucleotide excision repair.
  6. Exploring damage recognition models in prokaryotic nucleotide excision repair with a benzo[a]pyrene-derived lesion in UvrB.
    Title: Exploring damage recognition models in prokaryotic nucleotide excision repair with a benzo[a]pyrene-derived lesion in UvrB.
  7. N-terminus verified by Edman degradation on mature peptide
    Title: Sequences of the E. coli uvrB gene and protein.
  8. In solution, two UvrB subunits bind to UvrA, most likely as part of a UvrA2B2 complex. The UvrA-induced dimerization of the C-terminal domain 4 regions of UvrB serves to shield these domains from premature UvrC binding.
    Title: Dynamics of the UvrABC nucleotide excision repair proteins analyzed by fluorescence resonance energy transfer.
  9. transcription-repair ATP-dependent coupling factor-N reveals a fold that resembles the three N-terminal domains of the repair enzyme UvrB
    Title: Structural basis for transcription-coupled repair: the N terminus of Mfd resembles UvrB with degenerate ATPase motifs.
  10. we examined the interaction of UvrABC with a DNA substrate containing a single G[8,5-Me]T cross-link and compared it with T[6,4]T (the 6-4 pyrimidine-pyrimidone photoproduct) and the C8 guanine adduct of N-acetyl-2-aminofluorene (AAF)
    Title: Recognition and incision of gamma-radiation-induced cross-linked guanine-thymine tandem lesion G[8,5-Me]T by UvrABC nuclease.
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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables excinuclease ABC activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables nuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in SOS response IEA
Inferred from Electronic Annotation
more info
  
involved_in nucleotide-excision repair IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in nucleotide-excision repair IEA
Inferred from Electronic Annotation
more info
  
involved_in nucleotide-excision repair NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in nucleotide-excision repair, DNA damage recognition IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in nucleotide-excision repair, DNA damage recognition IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in nucleotide-excision repair, preincision complex assembly IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within response to radiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
part_of excinuclease repair complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of excinuclease repair complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of excinuclease repair complex IEA
Inferred from Electronic Annotation
more info
  
part_of excinuclease repair complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
UvrABC excision nuclease subunit B

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    813526..815547
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415300.1 UvrABC excision nuclease subunit B [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415300.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S7FF61
    Conserved Domains (1) summary
    PRK05298
    Location:1 → 673
    PRK05298; excinuclease ABC subunit UvrB
Nucleotide Protein
Heading Accession and Version

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