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rihA pyrimidine-specific ribonucleoside hydrolase RihA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945503, updated on 3-Dec-2024

Summary

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Official Symbol
rihA
Official Full Name
pyrimidine-specific ribonucleoside hydrolase RihA
Primary source
ECOCYC:G6358
Locus tag
b0651
See related
ASAP:ABE-0002227
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0644; ybeK
Summary
The rihA gene encodes a ribonucleoside hydrolase that preferentially utilizes cytidine and uridine . [More information is available at EcoCyc: G6358].
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Genomic context

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Sequence:
NC_000913.3 (683477..684412, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF1266 domain-containing protein YbeU Neighboring gene co-chaperone DjlC Neighboring gene chaperone protein HscC Neighboring gene glutamate/aspartate ABC transporter ATP binding subunit Neighboring gene glutamate/aspartate ABC transporter membrane subunit GltK Neighboring gene glutamate/aspartate ABC transporter membrane subunit GltJ

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Active site plasticity revealed from the structure of the enterobacterial N-ribohydrolase RihA bound to a competitive inhibitor. Garau G, et al. BMC Struct Biol, 2010 Jun 8. PMID 20529317, Free PMC Article
  2. New insights into the mechanism of nucleoside hydrolases from the crystal structure of the Escherichia coli YbeK protein bound to the reaction product. Muzzolini L, et al. Biochemistry, 2006 Jan 24. PMID 16411753
  3. The RihA, RihB, and RihC ribonucleoside hydrolases of Escherichia coli. Substrate specificity, gene expression, and regulation. Petersen C, et al. J Biol Chem, 2001 Jan 12. PMID 11027694
  4. Bacterial evolution through the selective loss of beneficial Genes. Trade-offs in expression involving two loci. Zinser ER, et al. Genetics, 2003 Aug. PMID 12930738, Free PMC Article
  5. A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Lasserre JP, et al. Electrophoresis, 2006 Aug. PMID 16858726

See all (8) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. the functional characterization of the Escherichia coli YbeK (RihA) protein as a pyrimidine nucleoside-preferring NH and its first crystal structure to 1.8 A resolution
    Title: New insights into the mechanism of nucleoside hydrolases from the crystal structure of the Escherichia coli YbeK protein bound to the reaction product.
Submit: New GeneRIF Correction

Pathways from PubChem

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General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables calcium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables hydrolase activity, acting on glycosyl bonds IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables purine nucleosidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ribosylpyrimidine nucleosidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ribosylpyrimidine nucleosidase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables uridine nucleosidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables uridine nucleosidase activity IEA
Inferred from Electronic Annotation
more info
  
enables uridine nucleosidase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in nucleobase-containing compound metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in nucleobase-containing small molecule interconversion IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within protein homotetramerization IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in purine nucleoside catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in pyrimidine nucleobase metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within pyrimidine ribonucleoside catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of protein-containing complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
pyrimidine-specific ribonucleoside hydrolase RihA
NP_415184.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    683477..684412 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415184.1 pyrimidine-specific ribonucleoside hydrolase RihA [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415184.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9ANV0
    Conserved Domains (1) summary
    PRK10443
    Location:1 → 311
    rihA; ribonucleoside hydrolase 1; Provisional
Nucleotide Protein
Heading Accession and Version

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