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putP proline:Na(+) symporter [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945602, updated on 3-Dec-2024

Summary

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Official Symbol
putP
Official Full Name
proline:Na(+) symporter
Primary source
ECOCYC:EG10802
Locus tag
b1015
See related
ASAP:ABE-0003433
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1006; putC
Summary
PutP is a member of the SSS family of sodium/solute transporters . [More information is available at EcoCyc: EG10802].
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Genomic context

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Sequence:
NC_000913.3 (1079305..1080813)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene fused DNA-binding transcriptional repressor/proline dehydrogenase/1-pyrroline-5-carboxylate dehydrogenase PutA Neighboring gene protein YmdG Neighboring gene pseudo Neighboring gene ferrous iron transport system protein EfeO

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Site-specific alteration of arginine 376, the unique positively charged amino acid residue in the mid-membrane-spanning regions of the proline carrier of Escherichia coli. Yamato I, et al. J Biol Chem, 1994 Feb 25. PMID 8119910
  2. Charge translocation during cosubstrate binding in the Na+/proline transporter of E.coli. Zhou A, et al. J Mol Biol, 2004 Oct 29. PMID 15476811
  3. Interresidual distance determination by four-pulse double electron-electron resonance in an integral membrane protein: the Na+/proline transporter PutP of Escherichia coli. Jeschke G, et al. Biophys J, 2004 Apr. PMID 15041691, Free PMC Article
  4. Sites important for Na+ and substrate binding in the Na+/proline transporter of Escherichia coli, a member of the Na+/solute symporter family. Pirch T, et al. J Biol Chem, 2002 Mar 15. PMID 11756453
  5. Core Transmembrane Domain 6 Plays a Pivotal Role in the Transport Cycle of the Sodium/Proline Symporter PutP. Bracher S, et al. J Biol Chem, 2016 Dec 9. PMID 27793991, Free PMC Article

See all (45) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. the results demonstrate that TM6' plays a central role in substrate binding and release on the inner side of the membrane also in PutP and extend the knowledge on functionally relevant amino acids in transporters with a LeuT-type structural fold.
    Title: Core Transmembrane Domain 6 Plays a Pivotal Role in the Transport Cycle of the Sodium/Proline Symporter PutP.
  2. Remarkably,as proposed computationally and discovered here experimentally, residues Y140, W244, and Y248 of transmembrane segments 4 and 7 are particularly important for PutP function and are suggested to participate in proline binding and/or gating.
    Title: Homology model of the Na+/proline transporter PutP of Escherichia coli and its functional implications.
  3. backbone models of PutP exhibiting a pronounced kink near residue T341, which is involved in substrate binding, were obtained. The kink may be associated with a hinge that allows the protein to open and close an inwardly oriented cavity.
    Title: Backbone structure of transmembrane domain IX of the Na+/proline transporter PutP of Escherichia coli.
  4. Results describe the function of transmembrane domain IX in the Na+/proline transporter PutP.
    Title: Function of transmembrane domain IX in the Na+/proline transporter PutP.
  5. N-terminus verified by Edman degradation on complete protein
    Title: Sodium ion and proline binding sites in the Na+/proline symport carrier of Escherichia coli.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables L-proline transmembrane transporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables proline:sodium symporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables proline:sodium symporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables proline:sodium symporter activity IEA
Inferred from Electronic Annotation
more info
  
enables sodium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables symporter activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in amino acid transport IEA
Inferred from Electronic Annotation
more info
  
involved_in carboxylic acid transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in carboxylic acid transport IEA
Inferred from Electronic Annotation
more info
  
involved_in proline transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in proline transport IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within proline transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in proline transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within proline transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within short-chain fatty acid transport IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within short-chain fatty acid transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in sodium ion transport IEA
Inferred from Electronic Annotation
more info
  
involved_in transmembrane transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 

General protein information

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Preferred Names
proline:Na(+) symporter

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1079305..1080813
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415535.1 proline:Na(+) symporter [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415535.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376YAE2
    Conserved Domains (1) summary
    PRK15419
    Location:1 → 502
    PRK15419; sodium/proline symporter PutP
Nucleotide Protein
Heading Accession and Version

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