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pspF DNA-binding transcriptional dual regulator PspF [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945683, updated on 3-Dec-2024

Summary

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Official Symbol
pspF
Official Full Name
DNA-binding transcriptional dual regulator PspF
Primary source
ECOCYC:EG12344
Locus tag
b1303
See related
ASAP:ABE-0004383
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1298; ycjB
Summary
The transcription factor PspF, for "Phage shock protein F," is a bacterial enhancer-binding protein required for 54-dependent transcription activation . [More information is available at EcoCyc: EG12344].
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Genomic context

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Sequence:
NC_000913.3 (1366935..1367912, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene gamma-glutamylputrescine oxidase Neighboring gene 4-aminobutyrate aminotransferase PuuE Neighboring gene phage shock protein A Neighboring gene phage shock protein B Neighboring gene phage shock protein C

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Nucleotide-dependent triggering of RNA polymerase-DNA interactions by an AAA regulator of transcription. Cannon W, et al. J Biol Chem, 2003 May 30. PMID 12649285
  2. Structural basis of the nucleotide driven conformational changes in the AAA+ domain of transcription activator PspF. Rappas M, et al. J Mol Biol, 2006 Mar 24. PMID 16430918
  3. 6S RNA regulation of pspF transcription leads to altered cell survival at high pH. Trotochaud AE, et al. J Bacteriol, 2006 Jun. PMID 16707685, Free PMC Article
  4. Evaluation of the inhibitory effect of antisense oligodeoxynucleotides on the growth of hepatitis C-associated hepatocellular carcinoma cells in vitro. Amin MA, et al. Chin J Dig Dis, 2005. PMID 16045605
  5. Structural insights into the activity of enhancer-binding proteins. Rappas M, et al. Science, 2005 Mar 25. PMID 15790859, Free PMC Article

See all (62) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. identified the minimal PspF-interacting domain of PspA, solved its structure, determined its affinity to PspF and the dissociation kinetics, identified residues that are potentially important for PspF regulation and analyzed effects of their mutation on PspF in vivo and in vitro
    Title: PspF-binding domain PspA1-144 and the PspA·F complex: New insights into the coiled-coil-dependent regulation of AAA+ proteins.
  2. By comparing the properties of the log and stationary phases, we find that the dynamics of PspF are influenced by the growth phase and may be controlled by the changes in the cytoplasmic fluidity.
    Title: Is the cellular and molecular machinery docile in the stationary phase of Escherichia coli?
  3. By structural modelling, authors propose that PspA binds to PspF via its first two helical domains.
    Title: A key hydrophobic patch identified in an AAA⁺ protein essential for its in trans inhibitory regulation.
  4. Identification of two previously unknown PspF loop 1 (L1)-binding patches within region 1 of sigma54.
    Title: A common feature from different subunits of a homomeric AAA+ protein contacts three spatially distinct transcription elements.
  5. The transcription activator PspF is a molecular model whose conserved amino acid residues have roles in ATP hydrolysis and intersubunit communication.
    Title: ATPase site architecture is required for self-assembly and remodeling activity of a hexameric AAA+ transcriptional activator.
  6. The phage shock protein (Psp) stress response system is an important part of the stress response machinery in Escherichia coli K12.
    Title: The evolution of the phage shock protein response system: interplay between protein function, genomic organization, and system function.
  7. Here the authors established that the PspA negative regulation of PspF ATPase activity is the result of a cooperative inhibition.
    Title: A lower-order oligomer form of phage shock protein A (PspA) stably associates with the hexameric AAA(+) transcription activator protein PspF for negative regulation.
  8. The pre-SIi loop in PspF is a major structural feature mediating nucleotide state-dependent differential engagement with Esigma54.
    Title: Functional roles of the pre-sensor I insertion sequence in an AAA+ bacterial enhancer binding protein.
  9. analysis of how PspF couples ATPase activity in AAA+ proteins for transcription activation
    Title: An intramolecular route for coupling ATPase activity in AAA+ proteins for transcription activation.
  10. A stochastic nucleotide binding and a coordinated hydrolysis mechanism in PspF1-275 hexamers is presented.
    Title: Heterogeneous nucleotide occupancy stimulates functionality of phage shock protein F, an AAA+ transcriptional activator.
Submit: New GeneRIF Correction See all GeneRIFs (15)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription activator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables cis-regulatory region sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables sequence-specific DNA binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within negative regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within negative regulation of DNA-templated transcription IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within negative regulation of DNA-templated transcription ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
involved_in phosphorelay signal transduction system IEA
Inferred from Electronic Annotation
more info
  
involved_in positive regulation of DNA-templated transcription IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within positive regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within positive regulation of DNA-templated transcription IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within positive regulation of DNA-templated transcription ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
involved_in regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of cellular response to stress NAS
Non-traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
part_of protein-DNA complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of transcription regulator complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
DNA-binding transcriptional dual regulator PspF

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1366935..1367912 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415819.4 DNA-binding transcriptional dual regulator PspF [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415819.4

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A2X1QER1, A0A8S7EFZ0
    Conserved Domains (1) summary
    PRK11608
    Location:1 → 325
    pspF; phage shock protein operon transcriptional activator; Provisional
Nucleotide Protein
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