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mqsA antitoxin of the MqsRA toxin-antitoxin system/DNA-binding transcriptional repressor MqsA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945814, updated on 17-Dec-2024

Summary

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Official Symbol
mqsA
Official Full Name
antitoxin of the MqsRA toxin-antitoxin system/DNA-binding transcriptional repressor MqsA
Primary source
ECOCYC:G7571
Locus tag
b3021
See related
ASAP:ABE-0009923
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3012; ygiT
Summary
Toxin MsqR could not be cloned in an ara vector unless antitoxin YgiT is upregulated (Shah, 2006). [More information is available at EcoGene: EG13022].
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Genomic context

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Sequence:
NC_000913.3 (3167851..3168246, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA topoisomerase IV subunit A Neighboring gene putative deoxycholate binding periplasmic protein Neighboring gene mRNA interferase toxin MqsR Neighboring gene DNA-binding transcriptional repressor YgiV

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Solution structure and biophysical properties of MqsA, a Zn-containing antitoxin from Escherichia coli. Papadopoulos E, et al. Biochim Biophys Acta, 2012 Dec. PMID 22789559
  2. Antitoxin MqsA helps mediate the bacterial general stress response. Wang X, et al. Nat Chem Biol, 2011 Jun. PMID 21516113, Free PMC Article
  3. MqsR is a noncanonical microbial RNase toxin that is inhibited by antitoxin MqsA via steric blockage of substrate binding. Yu V, et al. J Biol Chem, 2022 Nov. PMID 36162504, Free PMC Article
  4. MqsR, a crucial regulator for quorum sensing and biofilm formation, is a GCU-specific mRNA interferase in Escherichia coli. Yamaguchi Y, et al. J Biol Chem, 2009 Oct 16. PMID 19690171, Free PMC Article
  5. The Escherichia coli toxin MqsR destabilizes the transcriptional repression complex formed between the antitoxin MqsA and the mqsRA operon promoter. Brown BL, et al. J Biol Chem, 2013 Jan 11. PMID 23172222, Free PMC Article

See all (24) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. MqsR is a noncanonical microbial RNase toxin that is inhibited by antitoxin MqsA via steric blockage of substrate binding.
    Title: MqsR is a noncanonical microbial RNase toxin that is inhibited by antitoxin MqsA via steric blockage of substrate binding.
  2. findings show that the cyclic AMP receptor protein (CRP) indirectly upregulates mqsRA transcription; also show that CRP plays an important role in antibiotic persistence, which seems to be partially mediated through MqsRA
    Title: The cyclic AMP receptor protein (CRP) regulates mqsRA, coding for the bacterial toxin-antitoxin gene pair, in Escherichia coli.
  3. two well-defined domains of MqsA have almost identical structure in solution and in the two published crystal structures of dimeric MqsA bound to either MqsR or DNA
    Title: Solution structure and biophysical properties of MqsA, a Zn-containing antitoxin from Escherichia coli.
  4. Upon oxidative stress, MqsA is rapidly degraded by Lon protease, resulting in induction of rpoS; degradation of antitoxins during stress leads to a switch from the planktonic state (high motility) to the biofilm state (low motility).
    Title: Antitoxin MqsA helps mediate the bacterial general stress response.
  5. solvent content of the crystal was consistent with the presence of two MqsA molecules bound to the duplex DNA in the asymmetric unit
    Title: Preliminary crystallographic analysis of the Escherichia coli antitoxin MqsA (YgiT/b3021) in complex with mqsRA promoter DNA.
  6. The MqsR/MqsA TA system controls cell physiology via its own toxicity as well as through its regulation of another toxin, CspD.
    Title: Escherichia coli toxin/antitoxin pair MqsR/MqsA regulate toxin CspD.
  7. Here, we report multiple individual and complex three-dimensional structures of MqsR and its antitoxin MqsA (B3021), which reveal that MqsR:MqsA form a novel toxin:antitoxin (TA) pair
    Title: Three dimensional structure of the MqsR:MqsA complex: a novel TA pair comprised of a toxin homologous to RelE and an antitoxin with unique properties.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within regulation of DNA-templated transcription IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of DNA-templated transcription NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in single-species biofilm formation IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
part_of toxin-antitoxin complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
antitoxin of the MqsRA toxin-antitoxin system/DNA-binding transcriptional repressor MqsA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3167851..3168246 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417493.1 antitoxin of the MqsRA toxin-antitoxin system/DNA-binding transcriptional repressor MqsA [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417493.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0AAN3SGG2
    Conserved Domains (1) summary
    pfam15731
    Location:1 → 131
    MqsA_antitoxin; Antitoxin component of bacterial toxin-antitoxin system, MqsA
Nucleotide Protein
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