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pspA phage shock protein A [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945887, updated on 3-Dec-2024

Summary

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Official Symbol
pspA
Official Full Name
phage shock protein A
Primary source
ECOCYC:EG10776
Locus tag
b1304
See related
ASAP:ABE-0004387
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
cog; ECK1299
Summary
A pspA deletion grows slow below 8C (as enabled by transgenic Oleispira antarctica Cpn10/60). [More information is available at EcoGene: EG10776]. PspA is a negative transcriptional regulator of the psp operon that does not directly interact with DNA . [More information is available at EcoCyc: EG10776].
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Genomic context

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Sequence:
NC_000913.3 (1368079..1368747)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 4-aminobutyrate aminotransferase PuuE Neighboring gene DNA-binding transcriptional dual regulator PspF Neighboring gene phage shock protein B Neighboring gene phage shock protein C Neighboring gene phage shock protein D

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Structure and function of PspA and Vipp1 N-terminal peptides: Insights into the membrane stress sensing and mitigation. McDonald C, et al. Biochim Biophys Acta Biomembr, 2017 Jan. PMID 27806910
  2. Membrane Stored Curvature Elastic Stress Modulates Recruitment of Maintenance Proteins PspA and Vipp1. McDonald C, et al. mBio, 2015 Sep 1. PMID 26330516, Free PMC Article
  3. Signal sequence processing is required for the assembly of LamB trimers in the outer membrane of Escherichia coli. Carlson JH, et al. J Bacteriol, 1993 Jun. PMID 8501036, Free PMC Article
  4. Expression of the pspA gene stimulates efficient protein export in Escherichia coli. Kleerebezem M, et al. Mol Microbiol, 1993 Mar. PMID 8387148
  5. Dissipation of proton motive force is not sufficient to induce the phage shock protein response in Escherichia coli. Engl C, et al. Curr Microbiol, 2011 May. PMID 21259006, Free PMC Article

See all (62) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structure and function of Escherichia coli PspA and Synechocystis Vipp1 N-terminal peptides in the membrane stress sensing and mitigation have been described.
    Title: Structure and function of PspA and Vipp1 N-terminal peptides: Insights into the membrane stress sensing and mitigation.
  2. identified the minimal PspF-interacting domain of PspA, solved its structure, determined its affinity to PspF and the dissociation kinetics, identified residues that are potentially important for PspF regulation and analyzed effects of their mutation on PspF in vivo and in vitro
    Title: PspF-binding domain PspA1-144 and the PspA·F complex: New insights into the coiled-coil-dependent regulation of AAA+ proteins.
  3. Escherichia coli PspA has an enhanced sensitivity for curvature elastic (SCE) stress and a higher affinity for the membrane than Synechocystis Vipp1.
    Title: Membrane Stored Curvature Elastic Stress Modulates Recruitment of Maintenance Proteins PspA and Vipp1.
  4. The discrete spatial distribution and dynamics of PspA effector in lateral membrane regions depend on MreB and RodZ.
    Title: Anionic lipids and the cytoskeletal proteins MreB and RodZ define the spatio-temporal distribution and function of membrane stress controller PspA in Escherichia coli.
  5. By structural modelling, authors propose that PspA binds to PspF via its first two helical domains.
    Title: A key hydrophobic patch identified in an AAA⁺ protein essential for its in trans inhibitory regulation.
  6. Multiple signals are needed to induce the Psp response.
    Title: Dissipation of proton motive force is not sufficient to induce the phage shock protein response in Escherichia coli.
  7. Here the authors established that the PspA negative regulation of PspF ATPase activity is the result of a cooperative inhibition.
    Title: A lower-order oligomer form of phage shock protein A (PspA) stably associates with the hexameric AAA(+) transcription activator protein PspF for negative regulation.
  8. PspA and PspG are localized to the cell poles and traffic between both poles.
    Title: In vivo localizations of membrane stress controllers PspA and PspG in Escherichia coli.
  9. PspA can form hollow spherical or prolate spheroidal particles of about 30-40nm diameter with a scaffold-like arrangement of protein subunits at the surface.
    Title: PspA can form large scaffolds in Escherichia coli.
  10. These results indicate that oligomers of PspA bind to membrane phospholipids and suppress proton leakage.
    Title: Escherichia coli phage-shock protein A (PspA) binds to membrane phospholipids and repairs proton leakage of the damaged membranes.
Submit: New GeneRIF Correction See all GeneRIFs (13)

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables phospholipid binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in phage shock IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within phage shock IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of cellular response to stress NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within response to heat IEP
Inferred from Expression Pattern
more info
 PubMed 
Component Evidence Code Pubs
located_in cell pole IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
colocalizes_with cytoplasmic side of plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
part_of transcription regulator complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
phage shock protein A

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1368079..1368747
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415820.1 phage shock protein A [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415820.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376M881, A0A376VK41, A0A8S7CFI5
    Conserved Domains (1) summary
    PRK10698
    Location:1 → 222
    PRK10698; phage shock protein PspA; Provisional
Nucleotide Protein
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