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fnr DNA-binding transcriptional dual regulator FNR [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945908, updated on 17-Dec-2024

Summary

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Official Symbol
fnr
Official Full Name
DNA-binding transcriptional dual regulator FNR
Primary source
ECOCYC:EG10325
Locus tag
b1334
See related
ASAP:ABE-0004475
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1330; nirA; nirR; ossA; oxrA
Summary
FNR represses genes involved in aerobic respiration and activates genes required for anaerobic respiration. [More information is available at EcoGene: EG10325]. FNR is a dual DNA-binding transcriptional regulator which stimulates the transcription of many genes required for fermentation and anaerobic respiration. [More information is available at EcoCyc: EG10325].
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Genomic context

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Sequence:
NC_000913.3 (1398774..1399526, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF2534 domain-containing protein YnaJ Neighboring gene universal stress protein E Neighboring gene methylated-DNA--[protein]-cysteine S-methyltransferase Neighboring gene p-aminobenzoyl glutamate:H(+) symporter

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. [Computer analysis of regulatory signals in bacterial genomes. Fnr binding segments]. Gerasimova AV, et al. Mol Biol (Mosk), 2001 Nov-Dec. PMID 11771123
  2. Iron-sulfur cluster disassembly in the FNR protein of Escherichia coli by O2: [4Fe-4S] to [2Fe-2S] conversion with loss of biological activity. Khoroshilova N, et al. Proc Natl Acad Sci U S A, 1997 Jun 10. PMID 9177174, Free PMC Article
  3. The oxygen-responsive transcriptional regulator FNR of Escherichia coli: the search for signals and reactions. Unden G, et al. Mol Microbiol, 1997 Jul. PMID 9282732
  4. Transcription activation at class I FNR-dependent promoters: identification of the activating surface of FNR and the corresponding contact site in the C-terminal domain of the RNA polymerase alpha subunit. Williams SM, et al. Nucleic Acids Res, 1997 Oct 15. PMID 9321653, Free PMC Article
  5. Transcription activation by Escherichia coli FNR protein: similarities to, and differences from, the CRP paradigm. Li B, et al. Nucleic Acids Res, 1998 May 1. PMID 9547262, Free PMC Article

See all (159) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The FNR F186I variant is apparently capable of enhancing resistance to ionizing radiation under chronic irradiation conditions, but does not increase cell survival when exposed to acute irradiation.
    Title: A variant of the Escherichia coli anaerobic transcription factor FNR exhibiting diminished promoter activation function enhances ionizing radiation resistance.
  2. Elevated expression of GlpT and UhpT via FNR activation contributes to increased fosfomycin susceptibility in Escherichia coli under anaerobic conditions.
    Title: Elevated Expression of GlpT and UhpT via FNR Activation Contributes to Increased Fosfomycin Susceptibility in Escherichia coli under Anaerobic Conditions.
  3. These results demonstrate that FNR is a key global regulator of uropathogenic Escherichia coli virulence and controls expression of important virulence factors that contribute to uropathogenic Escherichia coli pathogenicity.
    Title: FNR regulates expression of important virulence factors contributing to pathogenicity of uropathogenic Escherichia coli.
  4. DNA-bound FNR reacts more rapidly with O2 than FNR free in solution, implying that transcriptionally active FNR is the preferred target for reaction with O2.
    Title: Influence of association state and DNA binding on the O₂-reactivity of [4Fe-4S] fumarate and nitrate reduction (FNR) regulator.
  5. FNR bound some promoters without regulating expression presumably requiring changes in activity of condition-specific transcription factors. Such combinatorial regulation may allow Escherichia coli to respond rapidly to environmental changes
    Title: Genome-scale analysis of escherichia coli FNR reveals complex features of transcription factor binding.
  6. oxygen stable FNR variants were obtained.
    Title: Covalently linking the Escherichia coli global anaerobic regulator FNR in tandem allows it to function as an oxygen stable dimer.
  7. The authors characterized the steady-state behavior of the FNR system by determining its sensitivity to changes in parameter values and its response to changes in the concentration of iron-sulfur cluster assembly proteins and the protease ClpXP.
    Title: Regulation of aerobic-to-anaerobic transitions by the FNR cycle in Escherichia coli.
  8. The authors show that the global transcription factor FNR is a class II activator at the ynfEFGHI promoter and that NarL represses activation by binding to a single target that overlaps the promoter -10 element.
    Title: Activation and repression at the Escherichia coli ynfEFGHI operon promoter.
  9. Results suggest that the Fnr and Crp proteins may act synergistically to enhance NapABC synthesis during growth with poor carbon sources to help obtain energy from low levels of nitrate.
    Title: Catabolite repression control of napF (periplasmic nitrate reductase) operon expression in Escherichia coli K-12.
  10. transcriptomic data identified the global transcription regulators ArcA, CRP, Fis, FNR, Fur, BaeR, CpxR, and IHF as targets and potential CO sensors
    Title: Carbon monoxide-releasing antibacterial molecules target respiration and global transcriptional regulators.
Submit: New GeneRIF Correction See all GeneRIFs (21)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 4 iron, 4 sulfur cluster binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables 4 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription activator activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables DNA-binding transcription activator activity IPI
Inferred from Physical Interaction
more info
 PubMed 
enables DNA-binding transcription factor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA-binding transcription factor activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA-binding transcription factor activity IEA
Inferred from Electronic Annotation
more info
  
enables iron-sulfur cluster binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables transcription cis-regulatory region binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables transcription cis-regulatory region binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within anaerobic respiration IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in positive regulation of DNA-templated transcription EXP
Inferred from Experiment
more info
 PubMed 
involved_in positive regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within regulation of DNA-templated transcription IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within response to nitric oxide IEP
Inferred from Expression Pattern
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of protein-DNA complex IMP
Inferred from Mutant Phenotype
more info
 PubMed 
part_of protein-DNA complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
DNA-binding transcriptional dual regulator FNR

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1398774..1399526 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415850.1 DNA-binding transcriptional dual regulator FNR [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415850.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A2X3K777
    Conserved Domains (1) summary
    PRK11161
    Location:11 → 245
    PRK11161; fumarate/nitrate reduction transcriptional regulator Fnr
Nucleotide Protein
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