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pntB pyridine nucleotide transhydrogenase subunit beta [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946144, updated on 17-Dec-2024

Summary

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Official Symbol
pntB
Official Full Name
pyridine nucleotide transhydrogenase subunit beta
Primary source
ECOCYC:EG10745
Locus tag
b1602
See related
ASAP:ABE-0005352
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1597
Summary
[More information is available at EcoGene: EG10745]. PntB is an inner membrane protein with nine predicted transmembrane domains. [More information is available at EcoCyc: EG10745].
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Genomic context

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Sequence:
NC_000913.3 (1674972..1676360, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein MdtU Neighboring gene autoinducer 2 exporter Neighboring gene pyridine nucleotide transhydrogenase subunit alpha Neighboring gene DUF1471 domain-containing protein YdgH

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Glutamine 132 in the NAD(H)-binding component of proton-translocating transhydrogenase tethers the nucleotides before hydride transfer. van Boxel GI, et al. Biochemistry, 2003 Feb 11. PMID 12564924
  2. The crystal structure of an asymmetric complex of the two nucleotide binding components of proton-translocating transhydrogenase. Cotton NP, et al. Structure, 2001 Feb 7. PMID 11250201
  3. The reduction of acetylpyridine adenine dinucleotide by NADH: is it a significant reaction of proton-translocating transhydrogenase, or an artefact? Stilwell SN, et al. Biochim Biophys Acta, 1997 May 16. PMID 9186780
  4. Assembly of multimeric proteins. Effect of mutations in the alpha-subunit on membrane assembly and activity of pyridine nucleotide transhydrogenase. Ahmad S, et al. J Mol Biol, 1993 Nov 5. PMID 8230209
  5. Site-directed mutagenesis of tyrosine residues at nicotinamide nucleotide binding sites of Escherichia coli transhydrogenase. Olausson T, et al. Biochemistry, 1993 Dec 7. PMID 8241179

See all (119) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Distinct transcription factors regulated the PntA, PntB and UdhA promoters.
    Title: Distinct transcriptional regulation of the two Escherichia coli transhydrogenases PntAB and UdhA.
  2. N-terminus verified by Edman degradation on complete protein
    Title: Nucleotide sequence of the pntA and pntB genes encoding the pyridine nucleotide transhydrogenase of Escherichia coli.
  3. The essential Asp392 is shown to be protonated at neutral pH in both the NADP(+) and NADPH forms of domain III, but with a marked difference in pK(a) Titrating residues found in loop D/alpha5 point to a conformational difference that is redox-dependent.
    Title: Titration of E. coli transhydrogenase domain III with bound NADP+ or NADPH studied by NMR reveals no pH-dependent conformational change in the physiological pH range.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables NADP binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables proton-translocating NAD(P)+ transhydrogenase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables proton-translocating NAD(P)+ transhydrogenase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in NADPH regeneration IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in NADPH regeneration IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in proton export across plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISM
Inferred from Sequence Model
more info
 PubMed 

General protein information

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Preferred Names
pyridine nucleotide transhydrogenase subunit beta
NP_416119.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1674972..1676360 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416119.1 pyridine nucleotide transhydrogenase subunit beta [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416119.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376J522
    Conserved Domains (1) summary
    PRK09444
    Location:1 → 462
    pntB; Re/Si-specific NAD(P)(+) transhydrogenase subunit beta
Nucleotide Protein
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Gene LinkOut

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