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clcA chloride:H(+) antiporter ClcA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946715, updated on 3-Dec-2024

Summary

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Official Symbol
clcA
Official Full Name
chloride:H(+) antiporter ClcA
Primary source
ECOCYC:EG12331
Locus tag
b0155
See related
ASAP:ABE-0000533
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0154; eriC; yadQ
Summary
ClcA and ClcB encode redundant chloride channels. [More information is available at EcoGene: EG12331]. EriC is a homologue of ClC chloride channels. [More information is available at EcoCyc: EG12331].
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Genomic context

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Sequence:
NC_000913.3 (175107..176528)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene glutamate-1-semialdehyde 2,1-aminomutase Neighboring gene protein YadX Neighboring gene protein YadW Neighboring gene iron-sulfur cluster insertion protein ErpA Neighboring gene PF03458 family protein YadS

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Conformational changes required for H(+)/Cl(-) exchange mediated by a CLC transporter. Basilio D, et al. Nat Struct Mol Biol, 2014 May. PMID 24747941, Free PMC Article
  2. Gating the selectivity filter in ClC chloride channels. Dutzler R, et al. Science, 2003 Apr 4. PMID 12649487
  3. Structure and function of clc channels. Chen TY. Annu Rev Physiol, 2005. PMID 15709979
  4. X-ray structure of a ClC chloride channel at 3.0 A reveals the molecular basis of anion selectivity. Dutzler R, et al. Nature, 2002 Jan 17. PMID 11796999
  5. Side-dependent inhibition of a prokaryotic ClC by DIDS. Matulef K, et al. Biophys J, 2005 Sep. PMID 15994902, Free PMC Article

See all (36) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Thermodynamic analysis of ion and proton binding shows how the structure of the CLC transporter effects the pH-independent, 2 Cl- to 1 proton binding stoichiometry. The key features are a helix cage, which creates a compact region with a very strong electropositive potential that stabilizes two anions and a single Glutamate that competes with two Cl ions.
    Title: Two Cl Ions and a Glu Compete for a Helix Cage in the CLC Proton/Cl(-) Antiporter.
  2. The authors propose that CLC-ec1 operates through an affinity-switch mechanism and that the bases of substrate specificity are conserved in the CLC channels and transporters.
    Title: Basis of substrate binding and conservation of selectivity in the CLC family of channels and transporters.
  3. E203 substitution with Cys and crystal structures of mutants showed that respective Glu is the internal proton-transfer residue that delivers protons from intracellular solution to the protein interior
    Title: Intracellular proton-transfer mutants in a CLC Cl-/H+ exchanger.
  4. Crystallographic study on the ion-binding properties of the ClC selectivity filter in the close homolog from Escherichia coli was presented.
    Title: Ion-binding properties of the ClC chloride selectivity filter.
  5. The unitary Cl(-) transport rate, measured in liposomes by both initial-velocity method and a novel Poisson dilution approach, is approximately 4,000 s(-1) for wild-type protein, and the uncoupled mutants transport Cl(-) at similar rates
    Title: Uncoupling and turnover in a Cl-/H+ exchange transporter.
Submit: New GeneRIF Correction

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables antiporter activity IEA
Inferred from Electronic Annotation
more info
  
enables chloride:proton antiporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables voltage-gated chloride channel activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables voltage-gated chloride channel activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in cellular stress response to acidic pH IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in chloride transmembrane transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in chloride transport IEA
Inferred from Electronic Annotation
more info
  
involved_in monoatomic ion transport IEA
Inferred from Electronic Annotation
more info
  
involved_in proton transmembrane transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISM
Inferred from Sequence Model
more info
 PubMed 

General protein information

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Preferred Names
chloride:H(+) antiporter ClcA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    175107..176528
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414697.1 chloride:H(+) antiporter ClcA [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414697.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A6C9Q5G3, A0A8S7TIM9
    Conserved Domains (1) summary
    PRK05277
    Location:36 → 473
    PRK05277; H(+)/Cl(-) exchange transporter ClcA
Nucleotide Protein
Heading Accession and Version

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