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mepA peptidoglycan DD-endopeptidase/peptidoglycan LD-endopeptidase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946812, updated on 3-Dec-2024

Summary

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Official Symbol
mepA
Official Full Name
peptidoglycan DD-endopeptidase/peptidoglycan LD-endopeptidase
Primary source
ECOCYC:EG10580
Locus tag
b2328
See related
ASAP:ABE-0007691
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2322
Summary
MepA is a penicillin-insensitive D-alanyl-D-alanine (DD) endopeptidase responsible for hydrolyzing D-alanyl-2,6-diaminopimelyl (DAP) as well as DAP-DAP (LD) amide bonds within cell wall peptidoglycan peptide crosslinks. [More information is available at EcoCyc: EG10580].
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Genomic context

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Sequence:
NC_000913.3 (2445560..2446384, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene EF-P-Lys34 hydroxylase Neighboring gene putative transporter YfcA Neighboring gene chorismate synthase Neighboring gene ribosomal protein L3 N(5)-glutamine methyltransferase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Mutational analysis of peptidoglycan amidase MepA. Firczuk M, et al. Biochemistry, 2007 Jan 9. PMID 17198381
  2. Purification of penicillin-insensitive DD-endopeptidase, a new cell wall peptidoglycan-hydrolyzing enzyme in Escherichia coli, and its inhibition by deoxyribonucleic acids. Tomioka S, et al. Biochem Biophys Res Commun, 1978 Oct 30. PMID 365181
  3. Escherichia coli murein-DD-endopeptidase insensitive to beta-lactam antibiotics. Keck W, et al. J Bacteriol, 1979 Sep. PMID 383691, Free PMC Article
  4. Evidence for multisite growth of Escherichia coli murein involving concomitant endopeptidase and transpeptidase activities. Burman LG, et al. J Bacteriol, 1983 Oct. PMID 6352683, Free PMC Article
  5. The architecture of the murein (peptidoglycan) in gram-negative bacteria: vertical scaffold or horizontal layer(s)? Vollmer W, et al. J Bacteriol, 2004 Sep. PMID 15342566, Free PMC Article

See all (31) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on mature peptide
    Title: Cloning and characterization of mepA, the structural gene of the penicillin-insensitive murein endopeptidase from Escherichia coli.
  2. MepA-type peptidases are LAS enzymes
    Title: Peptidoglycan amidase MepA is a LAS metallopeptidase.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables endopeptidase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metalloendopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables metallopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables peptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables peptidase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables serine-type endopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within peptidoglycan biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within peptidoglycan metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in peptidoglycan metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within peptidoglycan metabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in proteolysis IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to xenobiotic stimulus IEP
Inferred from Expression Pattern
more info
 PubMed 
Component Evidence Code Pubs
located_in outer membrane-bounded periplasmic space IEA
Inferred from Electronic Annotation
more info
  
located_in periplasmic space IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
peptidoglycan DD-endopeptidase/peptidoglycan LD-endopeptidase

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2445560..2446384 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416831.1 peptidoglycan DD-endopeptidase/peptidoglycan LD-endopeptidase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416831.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8T5ZWZ1
    Conserved Domains (1) summary
    COG3770
    Location:1 → 274
    MepA; Murein endopeptidase [Cell wall/membrane/envelope biogenesis]
Nucleotide Protein
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