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glyA serine hydroxymethyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947022, updated on 3-Dec-2024

Summary

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Official Symbol
glyA
Official Full Name
serine hydroxymethyltransferase
Primary source
ECOCYC:EG10408
Locus tag
b2551
See related
ASAP:ABE-0008389
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2548
Summary
Serine hydroxymethyltransferase converts serine to glycine, transferring a methyl group to tetrahydrofolate, thus forming 5,10-methylene-tetrahydrofolate (5,10-mTHF). [More information is available at EcoCyc: EG10408].
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Genomic context

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Sequence:
NC_000913.3 (2684254..2685507, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF5107 domain-containing protein YphG Neighboring gene putative DNA-binding transcriptional regulator, NAGC-like Neighboring gene nitric oxide dioxygenase Neighboring gene nitrogen regulatory protein PII-1

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Studies on the regulation of one-carbon metabolism. II. Repression-derepression of serine hydroxymethyltransferase by methionine in Escherichia coli 113-3. Mansouri A, et al. J Biol Chem, 1972 Jan 25. PMID 4550600
  2. GLYCINE SYNTHESIS AND METABOLISM IN ESCHERICHIA COLI. PIZER LI. J Bacteriol, 1965 Apr. PMID 14276110, Free PMC Article
  3. Crystal structure at 2.4 A resolution of E. coli serine hydroxymethyltransferase in complex with glycine substrate and 5-formyl tetrahydrofolate. Scarsdale JN, et al. J Mol Biol, 2000 Feb 11. PMID 10656824
  4. MetR-mediated repression of the glyA gene in Escherichia coli. Lorenz E, et al. FEMS Microbiol Lett, 1996 Nov 1. PMID 8900067
  5. Escherichia coli cis- and trans-acting mutations that increase glyA gene expression. Lorenz E, et al. Mol Gen Genet, 1996 Jan 15. PMID 8569691

See all (58) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Inhibition of glycine cleavage system by pyridoxine 5'-phosphate causes synthetic lethality in glyA yggS and serA yggS in Escherichia coli.
    Title: Inhibition of glycine cleavage system by pyridoxine 5'-phosphate causes synthetic lethality in glyA yggS and serA yggS in Escherichia coli.
  2. A bioinformatic study shows that Tyr55 (at the active site) and Arg235 are strongly conserved within the serine hydroxymethyltransferase family and are involved in a cation-pi interaction in the large majority of available SHMT structures.
    Title: Role of a conserved active site cation-pi interaction in Escherichia coli serine hydroxymethyltransferase.
  3. N-terminus verified by Edman degradation on complete protein
    Title: The gcvB gene encodes a small untranslated RNA involved in expression of the dipeptide and oligopeptide transport systems in Escherichia coli.
  4. Results describe the mechanism of addition of pyridoxal 5'-phosphate to Escherichia coli apo-serine hydroxymethyltransferase.
    Title: The mechanism of addition of pyridoxal 5'-phosphate to Escherichia coli apo-serine hydroxymethyltransferase.
  5. activation of glyA by CsgD requires the participation of the regulatory proteins, MetR and PurR
    Title: Role of MetR and PurR in the activation of glyA by CsgD in Escherichia coli K-12.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables L-allo-threonine aldolase activity IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables glycine hydroxymethyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables glycine hydroxymethyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glycine hydroxymethyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables glycine hydroxymethyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyridoxal phosphate binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables pyridoxal phosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyridoxal phosphate binding IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within L-serine biosynthetic process IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within L-serine catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in glycine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in glycine biosynthetic process from serine IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within glycine biosynthetic process from serine IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in glycine biosynthetic process from serine IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within glycine biosynthetic process from serine IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within glycine catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in one-carbon metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in tetrahydrofolate interconversion IEA
Inferred from Electronic Annotation
more info
  
involved_in tetrahydrofolate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol HDA PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane HDA PubMed 

General protein information

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Preferred Names
serine hydroxymethyltransferase
NP_417046.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2684254..2685507 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417046.1 serine hydroxymethyltransferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417046.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S7T8K8, A0AAN5ZHF1
    Conserved Domains (1) summary
    PRK00011
    Location:6 → 417
    glyA; serine hydroxymethyltransferase; Reviewed
Nucleotide Protein
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