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lepA 30S ribosomal subunit biogenesis factor LepA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947051, updated on 3-Dec-2024

Summary

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Official Symbol
lepA
Official Full Name
30S ribosomal subunit biogenesis factor LepA
Primary source
ECOCYC:EG10529
Locus tag
b2569
See related
ASAP:ABE-0008452
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2567
Summary
Although EF4 improves translational accuracy, it can not reverse paromomycin-induced errors (Qin, 2006). [More information is available at EcoGene: EG10529]. LepA is an elongation factor that induces back-translocation of the ribosome. [More information is available at EcoCyc: EG10529].
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Genomic context

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Sequence:
NC_000913.3 (2705325..2707124, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 30S ribosomal subunit maturation GTPase Era Neighboring gene RNase III Neighboring gene signal peptidase I Neighboring gene protein RseC Neighboring gene anti-sigma factor stabilizing protein RseB Neighboring gene anti-sigma-E factor RseA

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Interrupted catalysis: the EF4 (LepA) effect on back-translocation. Liu H, et al. J Mol Biol, 2010 Mar 5. PMID 20045415, Free PMC Article
  2. Small clusters of divergent amino acids surrounding the effector domain mediate the varied phenotypes of EF-G and LepA expression. Yaskowiak ES, et al. Mol Microbiol, 1995 Mar. PMID 7596295
  3. The highly conserved LepA is a ribosomal elongation factor that back-translocates the ribosome. Qin Y, et al. Cell, 2006 Nov 17. PMID 17110332
  4. Ribosomal translocation: LepA does it backwards. Youngman EM, et al. Curr Biol, 2007 Feb 20. PMID 17307049
  5. The structure of LepA, the ribosomal back translocase. Evans RN, et al. Proc Natl Acad Sci U S A, 2008 Mar 25. PMID 18362332, Free PMC Article

See all (34) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The last 44 C-terminal amino acids of EF4 form a sub-domain within the C-terminal domain that is important for GTP-dependent function on the ribosome.
    Title: Identification of two structural elements important for ribosome-dependent GTPase activity of elongation factor 4 (EF4/LepA).
  2. Thus, EF4 has two opposing functions-at low-to-moderate levels of stress, the protein is protective by allowing stress-paused translation to resume; at high-levels of stress, EF4 helps bacteria self-destruct.
    Title: Ribosomal elongation factor 4 promotes cell death associated with lethal stress.
  3. (review) EF4 triggers back-translocation of the elongating ribosome, causing the translation machinery to move one codon backwards along the mRNA.
    Title: The paradox of elongation factor 4: highly conserved, yet of no physiological significance?
  4. Elevated intracellular Mg(2+) concentrations or low temperature inhibit protein synthesis, probably because of formation of aberrant elongating ribosomal states. We suggest that EF4 binds to these stuck ribosomes and remobilizes them
    Title: Elongation factor 4 (EF4/LepA) accelerates protein synthesis at increased Mg2+ concentrations.
  5. EF4-dependent back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex via a four-step kinetic scheme (i.e., POST-->I(1)-->I(2)-->I(3)-->PRE
    Title: Interrupted catalysis: the EF4 (LepA) effect on back-translocation.
  6. The crystal structure of LepA from Escherichia coli at 2.8-A resolution, was determined.
    Title: The structure of LepA, the ribosomal back translocase.
  7. LepA protein, present in all bacteria and mitochondria, is a third elongation factor required for accurate and efficient protein synthesis.
    Title: The highly conserved LepA is a ribosomal elongation factor that back-translocates the ribosome.
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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables GTP binding IEA
Inferred from Electronic Annotation
more info
  
enables GTPase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables GTPase activity IEA
Inferred from Electronic Annotation
more info
  
enables guanosine tetraphosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables guanosine tetraphosphate binding IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables membrane scission GTPase motor activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ribosomal large subunit binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ribosomal small subunit binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ribosome binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ribosome binding IEA
Inferred from Electronic Annotation
more info
  
enables translation elongation factor activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in positive regulation of translation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in positive regulation of translation IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to cold IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within response to pH IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within response to salt stress IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within ribosomal small subunit biogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in translation IEA
Inferred from Electronic Annotation
more info
  
involved_in translational elongation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
30S ribosomal subunit biogenesis factor LepA
NP_417064.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2705325..2707124 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417064.1 30S ribosomal subunit biogenesis factor LepA [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417064.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    W1F6E5
    Conserved Domains (1) summary
    PRK05433
    Location:1 → 597
    PRK05433; GTP-binding protein LepA; Provisional
Nucleotide Protein
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