U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

hypD Fe-(CN)2CO cofactor assembly scaffold protein HypD [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947189, updated on 3-Dec-2024

Summary

Go to the top of the page Help
Official Symbol
hypD
Official Full Name
Fe-(CN)2CO cofactor assembly scaffold protein HypD
Primary source
ECOCYC:EG10486
Locus tag
b2729
See related
ASAP:ABE-0008965
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2724; hydF
Summary
HypD is an accessory protein that is essential for synthesis of all three hydrogenase isoenzymes . [More information is available at EcoCyc: EG10486].
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Sequence:
NC_000913.3 (2852136..2853257)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene hydrogenase 3 nickel incorporation protein HypA Neighboring gene hydrogenase isoenzymes nickel incorporation protein HypB Neighboring gene hydrogenase maturation factor HypC Neighboring gene carbamoyl dehydratase HypE Neighboring gene DNA-binding transcriptional activator FhlA Neighboring gene protein YgbA

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. The iron-sulfur-containing HypC-HypD scaffold complex of the [NiFe]-hydrogenase maturation machinery is an ATPase. Nutschan K, et al. FEBS Open Bio, 2019 Dec. PMID 31614069, Free PMC Article
  2. HypD is the scaffold protein for Fe-(CN)2CO cofactor assembly in [NiFe]-hydrogenase maturation. Stripp ST, et al. Biochemistry, 2013 May 14. PMID 23597401
  3. Hydrogen metabolism in Escherichia coli: biochemical and genetic evidence for a hydF gene. Sankar P, et al. J Bacteriol, 1988 Dec. PMID 3056901, Free PMC Article
  4. Properties of the [NiFe]-hydrogenase maturation protein HypD. Blokesch M, et al. FEBS Lett, 2006 Jul 24. PMID 16814778
  5. New insights into [FeFe] hydrogenase activation and maturase function. Kuchenreuther JM, et al. PLoS One, 2012. PMID 23049878, Free PMC Article

See all (24) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. analysis of a cell-free approach for H-cluster synthesis and hydrogenase maturation by using separately expressed and purified HydE, HydF, and HydG
    Title: New insights into [FeFe] hydrogenase activation and maturase function.
  2. dynamic complex between the hydrogenase maturation proteins HypD, HypC, HypE and HypF, which is the site of ligand biosynthesis and attachment to the iron atom of the NiFe site in hydrogenase
    Title: The complex between hydrogenase-maturation proteins HypC and HypD is an intermediate in the supply of cyanide to the active site iron of [NiFe]-hydrogenases.
Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 4 iron, 4 sulfur cluster binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 4 iron, 4 sulfur cluster binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables 4 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables carbon monoxide binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables carbon monoxide binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables iron ion binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables iron ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in protein maturation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein maturation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein-containing complex assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein-containing complex assembly NAS
Non-traceable Author Statement
more info
 PubMed 

General protein information

Go to the top of the page Help
Preferred Names
Fe-(CN)2CO cofactor assembly scaffold protein HypD

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2852136..2853257
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417209.1 Fe-(CN)2CO cofactor assembly scaffold protein HypD [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417209.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376J219
    Conserved Domains (1) summary
    TIGR00075
    Location:2 → 369
    hypD; hydrogenase expression/formation protein HypD
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.