rpoS RNA polymerase sigma factor RpoS [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

Go to the top of the page Help

Official Symbol: rpoS
Official Full Name: RNA polymerase sigma factor RpoS
Primary source: ECOCYC:EG10510
Locus tag: b2741
See related: ASAP:ABE-0009001
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: abrD; appR; csi2; dpeB; ECK2736; katF; nur; otsX; sigS
Summary: Polyamine modulon. [More information is available at EcoGene: EG10510]. rpoS encodes the alternative sigma factor S, a subunit of RNA polymerase that acts as the master regulator of the general stress response in Escherichia coli. [More information is available at EcoCyc: EG10510].
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

Sequence:: NC_000913.3 (2866559..2867551, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Lack of polyamines leads to cotranslational degradation of the general stress factor RpoS in Escherichia coli.
Title: Lack of polyamines leads to cotranslational degradation of the general stress factor RpoS in Escherichia coli.
TorR/TorS Two-Component system resists extreme acid environment by regulating the key response factor RpoS in Escherichia coli.
Title: TorR/TorS Two-Component system resists extreme acid environment by regulating the key response factor RpoS in Escherichia coli.
Results find the protective heat shock response is dependent on the general stress response sigma factor, RpoS. This indicates a novel regulatory role for RpoS for mistranslation- induced heat shock response activation and a second benefit RpoS provides due to increased mistranslation rates in addition to protection against oxidative stress.
Title: Increased mistranslation protects E. coli from protein misfolding stress due to activation of a RpoS-dependent heat shock response.
The RpoS accumulation at high pH required the presence of one or more antiadaptors that block degradation.
Title: Experimental Evolution of Escherichia coli K-12 at High pH and with RpoS Induction.
ptsN is required for fitness under minimal medium and for the proper balance between RpoS and sigma 70 activities in such way that, in the absence of ptsN, RpoS-dependent genes are preferentially expressed.
Title: Proteome analysis of an Escherichia coli ptsN-null strain under different nitrogen regimes.
The authors have determined how ElaB is regulated under oxidative stress: RpoS and OxyR coordinately control the expression of inner membrane protein ElaB.
Title: Resistance to oxidative stress by inner membrane protein ElaB is regulated by OxyR and RpoS.
RpoS contributes to the resistance of E. coli to protozoan predation and that RpoS is crucial for the increased fitness of soil-persistent E. coli against predation and reduced moisture in soil.
Title: Roles for RpoS in survival of Escherichia coli during protozoan predation and in reduced moisture conditions highlight its importance in soil environments.
bolA plays a major role in the presence and absence of rpoS in attachment to the stainless steel and silicone surfaces.
Title: Role of bolA and rpoS genes in biofilm formation and adherence pattern by Escherichia coli K-12 MG1655 on polypropylene, stainless steel, and silicone surfaces.
RNA polymerase is pausing during initial transcription.
Title: RNA Polymerase Pausing during Initial Transcription.
Our study indicates that the RNA chaperone Hfq contributes to the persistent infection of E. coli by maintaining the expression of bacterial genes, including one coding for sigma(38), that help bacteria evade host immunity.
Title: Inhibition of Phagocytic Killing of Escherichia coli in Drosophila Hemocytes by RNA Chaperone Hfq.

Submit: New GeneRIF Correction See all GeneRIFs (83)

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables bacterial-type RNA polymerase core enzyme binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sigma factor activity	IDA Inferred from Direct Assay more info	PubMed
enables sigma factor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA-templated transcription	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA-templated transcription initiation	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA-templated transcription initiation	IEA Inferred from Electronic Annotation more info
involved_in DNA-templated transcription initiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of DNA-templated transcription	NAS Non-traceable Author Statement more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulation of DNA-templated transcription initiation	IEA Inferred from Electronic Annotation more info
involved_in regulation of DNA-templated transcription initiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of gene expression	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to stress	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of cytosolic DNA-directed RNA polymerase complex	ISS Inferred from Sequence or Structural Similarity more info
part_of sigma factor antagonist complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: RNA polymerase sigma factor RpoS

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

2866559..2867551 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_417221.1 RNA polymerase sigma factor RpoS [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_417221.1

Status: PROVISIONAL

UniProtKB/TrEMBL

G9J668

Conserved Domains (1) summary

PRK05657
Location:4 → 328

PRK05657; RNA polymerase sigma factor RpoS; Validated