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tsaD N(6)-L-threonylcarbamoyladenine synthase, TsaD subunit [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947578, updated on 3-Dec-2024

Summary

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Official Symbol
tsaD
Official Full Name
N(6)-L-threonylcarbamoyladenine synthase, TsaD subunit
Primary source
ECOCYC:EG11171
Locus tag
b3064
See related
ASAP:ABE-0010058
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3054; gcp; ygjD
Summary
The protein is essential for growth . [More information is available at EcoCyc: EG11171].
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Genomic context

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Sequence:
NC_000913.3 (3209530..3210543, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene L(+)-tartrate dehydratase subunit alpha Neighboring gene L(+)-tartrate dehydratase subunit beta Neighboring gene L-tartrate:succinate antiporter Neighboring gene 30S ribosomal subunit protein S21 Neighboring gene DNA primase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Effects on transcription of mutations in ygjD, yeaZ, and yjeE genes, which are involved in a universal tRNA modification in Escherichia coli. Hashimoto C, et al. J Bacteriol, 2011 Nov. PMID 21873492, Free PMC Article
  2. Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD. Bergmiller T, et al. BMC Microbiol, 2011 May 27. PMID 21619589, Free PMC Article
  3. The ubiquitous conserved glycopeptidase Gcp prevents accumulation of toxic glycated proteins. Katz C, et al. mBio, 2010 Aug 24. PMID 20824107, Free PMC Article
  4. Evidence for non-enzymatic glycosylation in Escherichia coli. Mironova R, et al. Mol Microbiol, 2001 Feb. PMID 11251824
  5. Evidence for non-enzymatic glycosylation of Escherichia coli chromosomal DNA. Mironova R, et al. Mol Microbiol, 2005 Mar. PMID 15752201

See all (22) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. in bacteria four proteins (YgjD, YrdC, YjeE, and YeaZ) are both necessary and sufficient for t(6)A biosynthesis in vitro
    Title: Biosynthesis of threonylcarbamoyl adenosine (t6A), a universal tRNA nucleoside.
  2. This study showed that the transcription of the thr and ilv operons in ygjD mutants was increased through the inhibition of transcription attenuation and that dnaG transcription was reduced.
    Title: Effects on transcription of mutations in ygjD, yeaZ, and yjeE genes, which are involved in a universal tRNA modification in Escherichia coli.
  3. Depletion of YgjD protein from growing cells leads to a decrease in cell size that is contingent on (p)ppGpp, and to a termination of cell division.
    Title: Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD.
  4. Escherichia coli containing a conserved Gcp prevents the accumulation of toxic glycated proteins.
    Title: The ubiquitous conserved glycopeptidase Gcp prevents accumulation of toxic glycated proteins.
  5. Repression of ygjD expression results in at least a proportion of very enlarged cells with an unusual peripheral distribution of DNA, showing that this gene is essential for the viability of E. coli.
    Title: Conserved network of proteins essential for bacterial viability.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables N(6)-L-threonylcarbamoyladenine synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables acyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables acyltransferase activity, transferring groups other than amino-acyl groups IEA
Inferred from Electronic Annotation
more info
  
enables glycosylation-dependent protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables iron ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables iron ion binding IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in maintenance of translational fidelity NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in tRNA modification IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA threonylcarbamoyladenosine metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within tRNA threonylcarbamoyladenosine modification IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in tRNA threonylcarbamoyladenosine modification IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in tRNA threonylcarbamoyladenosine modification IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within tRNA threonylcarbamoyladenosine modification IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of EKC/KEOPS complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
N(6)-L-threonylcarbamoyladenine synthase, TsaD subunit
NP_417536.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3209530..3210543 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417536.1 N(6)-L-threonylcarbamoyladenine synthase, TsaD subunit [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417536.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A3S4NRD4
    Conserved Domains (1) summary
    PRK09604
    Location:1 → 337
    PRK09604; tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD
Nucleotide Protein
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