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truB tRNA pseudouridine(55) synthase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947687, updated on 3-Dec-2024

Summary

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Official Symbol
truB
Official Full Name
tRNA pseudouridine(55) synthase
Primary source
ECOCYC:EG11177
Locus tag
b3166
See related
ASAP:ABE-0010404
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3155; yhbA
Summary
[More information is available at EcoGene: EG11177]. TruB catalyzes pseudouridylation at position 55 in tRNA . [More information is available at EcoCyc: EG11177].
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Genomic context

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Sequence:
NC_000913.3 (3311833..3312777, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene 30S ribosomal subunit protein S15 Neighboring gene 30S ribosome binding factor Neighboring gene translation initiation factor IF-2beta' Neighboring gene transcription termination/antitermination protein NusA

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. RNA modification enzyme TruB is a tRNA chaperone. Keffer-Wilkes LC, et al. Proc Natl Acad Sci U S A, 2016 Dec 13. PMID 27849601, Free PMC Article
  2. Deletion of the Escherichia coli pseudouridine synthase gene truB blocks formation of pseudouridine 55 in tRNA in vivo, does not affect exponential growth, but confers a strong selective disadvantage in competition with wild-type cells. Gutgsell N, et al. RNA, 2000 Dec. PMID 11142385, Free PMC Article
  3. Dissecting the roles of a strictly conserved tyrosine in substrate recognition and catalysis by pseudouridine 55 synthase. Phannachet K, et al. Biochemistry, 2005 Nov 29. PMID 16300397
  4. Precursor complex structure of pseudouridine synthase TruB suggests coupling of active site perturbations to an RNA-sequestering peripheral protein domain. Hoang C, et al. Protein Sci, 2005 Aug. PMID 15987897, Free PMC Article
  5. The roles of the essential Asp-48 and highly conserved His-43 elucidated by the pH dependence of the pseudouridine synthase TruB. Hamilton CS, et al. Arch Biochem Biophys, 2005 Jan 1. PMID 15581587

See all (26) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. we prove the tRNA chaperone activity of the pseudouridine synthase TruB, reveal its molecular mechanism, and demonstrate its importance for cellular fitness
    Title: RNA modification enzyme TruB is a tRNA chaperone.
  2. The single-round rate constant of pseudouridylation by TruB was determined to be 0.5 sec(-1).
    Title: Pre-steady-state kinetic analysis of the three Escherichia coli pseudouridine synthases TruB, TruA, and RluA reveals uniformly slow catalysis.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: Purification, cloning, and properties of the tRNA psi 55 synthase from Escherichia coli.
  4. Structural model of TruB in complex with a substrate RNA, suggests communication between the active site and the RNA-sequestering peripheral protein domain.
    Title: Precursor complex structure of pseudouridine synthase TruB suggests coupling of active site perturbations to an RNA-sequestering peripheral protein domain.
Submit: New GeneRIF Correction

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity, acting on RNA IEA
Inferred from Electronic Annotation
more info
  
enables isomerase activity IEA
Inferred from Electronic Annotation
more info
  
enables pseudouridine synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables pseudouridine synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables tRNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables tRNA pseudouridine synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables tRNA pseudouridine(55) synthase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in RNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in mRNA pseudouridine synthesis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in pseudouridine synthesis IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within tRNA folding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tRNA modification IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in tRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA pseudouridine synthesis IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within tRNA pseudouridine synthesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
tRNA pseudouridine(55) synthase
NP_417635.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3311833..3312777 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417635.1 tRNA pseudouridine(55) synthase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417635.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376M2A6
    Conserved Domains (1) summary
    PRK05033
    Location:3 → 314
    truB; tRNA pseudouridine synthase B; Provisional
Nucleotide Protein
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