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lptB lipopolysaccharide transport system ATP binding protein LptB [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947725, updated on 3-Dec-2024

Summary

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Official Symbol
lptB
Official Full Name
lipopolysaccharide transport system ATP binding protein LptB
Primary source
ECOCYC:EG11680
Locus tag
b3201
See related
ASAP:ABE-0010512
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3190; yhbG
Summary
LptB depletion results in rifampicin/bile salts sensitivity, indicative of an outer membrane integrity defect. [More information is available at EcoGene: EG11680]. LptB is an ATP-binding component of the LptAB-YrbK ABC transporter. [More information is available at EcoCyc: EG11680].
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Genomic context

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Sequence:
NC_000913.3 (3343944..3344669)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC Neighboring gene lipopolysaccharide transport system protein LptC Neighboring gene lipopolysaccharide transport system protein LptA Neighboring gene RNA polymerase sigma factor RpoN Neighboring gene ribosome hibernation-promoting factor

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. LptB-LptF coupling mediates the closure of the substrate-binding cavity in the LptB(2) FGC transporter through a rigid-body mechanism to extract LPS. Lundstedt EA, et al. Mol Microbiol, 2020 Aug. PMID 32236984, Free PMC Article
  2. Structural and functional studies of conserved nucleotide-binding protein LptB in lipopolysaccharide transport. Wang Z, et al. Biochem Biophys Res Commun, 2014 Sep 26. PMID 25172661
  3. Decoupling catalytic activity from biological function of the ATPase that powers lipopolysaccharide transport. Sherman DJ, et al. Proc Natl Acad Sci U S A, 2014 Apr 1. PMID 24639492, Free PMC Article
  4. The transmembrane α-helix of LptC participates in LPS extraction by the LptB(2) FGC transporter. Wilson A, et al. Mol Microbiol, 2022 Jul. PMID 35678757, Free PMC Article
  5. Identification of Residues in the Lipopolysaccharide ABC Transporter That Coordinate ATPase Activity with Extractor Function. Simpson BW, et al. mBio, 2016 Oct 18. PMID 27795402, Free PMC Article

See all (29) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The transmembrane alpha-helix of LptC participates in LPS extraction by the LptB2 FGC transporter.
    Title: The transmembrane α-helix of LptC participates in LPS extraction by the LptB(2) FGC transporter.
  2. LptB-LptF coupling mediates the closure of the substrate-binding cavity in the LptB2 FGC transporter through a rigid-body mechanism to extract LPS.
    Title: LptB-LptF coupling mediates the closure of the substrate-binding cavity in the LptB(2) FGC transporter through a rigid-body mechanism to extract LPS.
  3. Taken together, these data identify a specific binding site in LptB for the coupling helices of LptFG that is responsible for coupling of ATP hydrolysis by LptB with LptFG function to achieve lipopolysaccharide extraction.
    Title: Identification of Residues in the Lipopolysaccharide ABC Transporter That Coordinate ATPase Activity with Extractor Function.
  4. The two protomers of LptB have to work together for ATP hydrolysis and LPS transport.
    Title: Structural and functional studies of conserved nucleotide-binding protein LptB in lipopolysaccharide transport.
  5. both LptF and LptG formed a complex with LptB.
    Title: Biochemical characterization of an ABC transporter LptBFGC complex required for the outer membrane sorting of lipopolysaccharides.
  6. results suggest that LptB operate in the LPS assembly pathway and, together with other as-yet-unidentified components, could be part of a complex devoted to the transport of LPS from the periplasmic surface of the IM to the OM.
    Title: Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli.
  7. N-terminus verified by Edman degradation on mature peptide
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in Gram-negative-bacterium-type cell outer membrane assembly NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in lipopolysaccharide transport IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within lipopolysaccharide transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of ATP-binding cassette (ABC) transporter complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of ATP-binding cassette (ABC) transporter complex IEA
Inferred from Electronic Annotation
more info
  
part_of ATP-binding cassette (ABC) transporter complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in membrane NAS
Non-traceable Author Statement
more info
 PubMed 
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
part_of transporter complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
lipopolysaccharide transport system ATP binding protein LptB
NP_417668.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3343944..3344669
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417668.1 lipopolysaccharide transport system ATP binding protein LptB [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417668.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376VWU5
    Conserved Domains (1) summary
    PRK10895
    Location:1 → 241
    PRK10895; lipopolysaccharide ABC transporter ATP-binding protein; Provisional
Nucleotide Protein
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