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rplD 50S ribosomal subunit protein L4 [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947818, updated on 3-Dec-2024

Summary

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Official Symbol
rplD
Official Full Name
50S ribosomal subunit protein L4
Primary source
ECOCYC:EG10867
Locus tag
b3319
See related
ASAP:ABE-0010858
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3306; eryA
Summary
The L4 protein is a component of the 50S subunit of the ribosome and also regulates the expression of S10 operon at both the transcriptional and posttranscriptional level. [More information is available at EcoCyc: EG10867].
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Genomic context

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Sequence:
NC_000913.3 (3451681..3452286, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 30S ribosomal subunit protein S19 Neighboring gene 50S ribosomal subunit protein L2 Neighboring gene 50S ribosomal subunit protein L23 Neighboring gene 50S ribosomal subunit protein L3 Neighboring gene 30S ribosomal subunit protein S10

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Autogenous control of the S10 ribosomal protein operon of Escherichia coli: genetic dissection of transcriptional and posttranscriptional regulation. Freedman LP, et al. Proc Natl Acad Sci U S A, 1987 Sep. PMID 2442760, Free PMC Article
  2. A hairpin structure upstream of the terminator hairpin required for ribosomal protein L4-mediated attenuation control of the S10 operon of Escherichia coli. Zengel JM, et al. J Bacteriol, 1996 Apr. PMID 8636042, Free PMC Article
  3. Ribosomal protein L4 from Escherichia coli utilizes nonidentical determinants for its structural and regulatory functions. Li X, et al. RNA, 1996 Jan. PMID 8846294, Free PMC Article
  4. Changes in the level of poly(Phe) synthesis in Escherichia coli ribosomes containing mutants of L4 ribosomal protein from Thermus thermophilus can be explained by structural changes in the peptidyltransferase center: a molecular dynamics simulation analysis. Papadopoulos G, et al. Eur Biophys J, 2006 Oct. PMID 16773394
  5. Thermus thermophilus L4 ribosomal protein: purification and sensitivity alteration against erythromycin of E. coli cells harboring a single amino acid mutant of TthL4 within its extended loop. Leontiadou F, et al. Amino Acids, 2007 Sep. PMID 17048124

See all (68) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Posttranscriptional Regulation of tnaA by Protein-RNA Interaction Mediated by Ribosomal Protein L4 in Escherichia coli.
    Title: Posttranscriptional Regulation of tnaA by Protein-RNA Interaction Mediated by Ribosomal Protein L4 in Escherichia coli.
  2. The extended loops of ribosomal proteins L4 and L22 of Escherichia coli contribute to ribosome assembly and protein translation.
    Title: The extended loops of ribosomal proteins uL4 and uL22 of Escherichia coli contribute to ribosome assembly and protein translation.
  3. The effects of classical erythromycin resistance mutations in ribosomal proteins L4 and L22 of the large ribosomal subunit on the kinetics of erythromycin binding, were characterized.
    Title: Erythromycin resistance by L4/L22 mutations and resistance masking by drug efflux pump deficiency.
  4. Our results highlight the important role of L4 and L22 in ribosome function and assembly, and indicate that a variety of changes in these proteins can mediate macrolide resistance.
    Title: Novel mutations in ribosomal proteins L4 and L22 that confer erythromycin resistance in Escherichia coli.
  5. N-terminus verified by Edman degradation on complete protein
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA binding EXP
Inferred from Experiment
more info
 PubMed 
enables RNA-binding transcription regulator activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables endoribonuclease inhibitor activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables mRNA 5'-UTR binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables rRNA binding IEA
Inferred from Electronic Annotation
more info
  
enables structural constituent of ribosome IBA
Inferred from Biological aspect of Ancestor
more info
  
enables structural constituent of ribosome IEA
Inferred from Electronic Annotation
more info
  
enables translation repressor activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables translation repressor activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA-templated transcription termination IEA
Inferred from Electronic Annotation
more info
  
involved_in cytoplasmic translation NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in negative regulation of DNA-templated transcription IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within negative regulation of cytoplasmic translation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of translation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of translation IEA
Inferred from Electronic Annotation
more info
  
involved_in response to antibiotic IEA
Inferred from Electronic Annotation
more info
  
involved_in ribosome assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within transcriptional attenuation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in translation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of cytosolic large ribosomal subunit IDA
Inferred from Direct Assay
more info
 PubMed 
part_of cytosolic large ribosomal subunit IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of large ribosomal subunit IMP
Inferred from Mutant Phenotype
more info
 PubMed 
part_of ribonucleoprotein complex IEA
Inferred from Electronic Annotation
more info
  
located_in ribosome IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
50S ribosomal subunit protein L4

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3451681..3452286 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417778.1 50S ribosomal subunit protein L4 [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417778.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9AQ98
    Conserved Domains (1) summary
    PRK05319
    Location:1 → 201
    rplD; 50S ribosomal protein L4; Provisional
Nucleotide Protein
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