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mnmG 5-carboxymethylaminomethyluridine-tRNA synthase subunit MnmG [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948248, updated on 3-Dec-2024

Summary

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Official Symbol
mnmG
Official Full Name
5-carboxymethylaminomethyluridine-tRNA synthase subunit MnmG
Primary source
ECOCYC:EG10375
Locus tag
b3741
See related
ASAP:ABE-0012231
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3734; gidA; trmF
Summary
mnmG function is based on genetic experiments showing tht it is in the same pathway as mnmE and that mnmE is epistatic to mnmG. [More information is available at EcoGene: EG10375]. MnmG and MnmE both act in a tRNA modification pathway that reduces +2 frameshift errors in translation . [More information is available at EcoCyc: EG10375].
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Genomic context

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Sequence:
NC_000913.3 (3923744..3925633, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene ATP synthase accessory factor AtpI Neighboring gene 16S rRNA m(7)G527 methyltransferase Neighboring gene flavoprotein MioC Neighboring gene DNA-binding transcriptional dual regulator AsnC

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Structure-function analysis of Escherichia coli MnmG (GidA), a highly conserved tRNA-modifying enzyme. Shi R, et al. J Bacteriol, 2009 Dec. PMID 19801413, Free PMC Article
  2. Nucleotide sequence of Escherichia coli K-12 replication origin. Sugimoto K, et al. Proc Natl Acad Sci U S A, 1979 Feb. PMID 370832, Free PMC Article
  3. Concurrent transcription from the gid and mioC promoters activates replication of an Escherichia coli minichromosome. Ogawa T, et al. Mol Gen Genet, 1991 Nov. PMID 1745229
  4. The AT richness and gid transcription determine the left border of the replication origin of the E. coli chromosome. Asai T, et al. EMBO J, 1990 Dec. PMID 2249664, Free PMC Article
  5. Stabilization of G domain conformations in the tRNA-modifying MnmE-GidA complex observed with double electron electron resonance spectroscopy. Böhme S, et al. J Biol Chem, 2010 May 28. PMID 20353943, Free PMC Article

See all (34) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. deletion of the mnmE or mnmG gene results in complete inhibition of growth unless the medium contains polyamines
    Title: Absolute requirement for polyamines for growth of Escherichia coli mutants (mnmE/G) defective in modification of the wobble anticodon of transfer-RNA.
  2. Investigation of the activities and the tRNA specificity of the MnmG enzyme.
    Title: The output of the tRNA modification pathways controlled by the Escherichia coli MnmEG and MnmC enzymes depends on the growth conditions and the tRNA species.
  3. GidA binding induces large conformational and dynamic changes in MnmE
    Title: Stabilization of G domain conformations in the tRNA-modifying MnmE-GidA complex observed with double electron electron resonance spectroscopy.
  4. Limited trypsinolysis of MnmG suggests significant conformational changes upon FAD binding.
    Title: Structure-function analysis of Escherichia coli MnmG (GidA), a highly conserved tRNA-modifying enzyme.
  5. MnmE and GidA form a functional complex in the tRNA modification process in which both proteins are interdependent.
    Title: Further insights into the tRNA modification process controlled by proteins MnmE and GidA of Escherichia coli.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables flavin adenine dinucleotide binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables flavin adenine dinucleotide binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables flavin adenine dinucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in regulation of cytoplasmic translational fidelity NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within response to UV IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tRNA methylation IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within tRNA methylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in tRNA wobble uridine modification IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within tRNA wobble uridine modification IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in tRNA wobble uridine modification IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within tRNA wobble uridine modification IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of cytosolic tRNA wobble base thiouridylase complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
5-carboxymethylaminomethyluridine-tRNA synthase subunit MnmG

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3923744..3925633 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418197.1 5-carboxymethylaminomethyluridine-tRNA synthase subunit MnmG [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418197.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A377C9H7, A0A8S7FB94
    Conserved Domains (1) summary
    PRK05192
    Location:3 → 628
    PRK05192; tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG
Nucleotide Protein
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