glnA glutamine synthetase [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: glnA
Official Full Name: glutamine synthetase
Primary source: ECOCYC:EG10383
Locus tag: b3870
See related: ASAP:ABE-0012640
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK3863
Summary: Induced during biofilm formation. [More information is available at EcoGene: EG10383]. Glutamine synthetase catalyzes the lone reaction in the glutamine biosynthesis pathway, combining L-glutamate and ammonia to generate L-glutamine. [More information is available at EcoCyc: EG10383].
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Genomic context

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Sequence:: NC_000913.3 (4056625..4058034, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

First description of a clinical glutamine-dependent Escherichia coli with a missense mutation in the glnA.
Title: First description of a clinical glutamine-dependent Escherichia coli with a missense mutation in the glnA.
Assembly status transition offers an avenue for activity modulation of a supramolecular enzyme.
Title: Assembly status transition offers an avenue for activity modulation of a supramolecular enzyme.
investigation of regulation of UR-PII-ATase-GS bicyclic system: Data suggest that AT activity is only bound/activated by completely unmodified PII and that this design is largely responsible for ultrasensitivity of bicyclic system.
Title: A source of ultrasensitivity in the glutamine response of the bicyclic cascade system controlling glutamine synthetase adenylylation state and activity in Escherichia coli.
Reversible adenylylation of glutamine synthetase is dynamically counterbalanced during steady-state growth.
Title: Reversible adenylylation of glutamine synthetase is dynamically counterbalanced during steady-state growth of Escherichia coli.
N-terminus verified by Edman degradation on mature peptide
Title: '98 Escherichia coli SWISS-2DPAGE database update.
binding site for FIS (factor for inversion stimulation) was identified upstream of a sigma54-dependent promoter, glnAp2; binding of FIS at this site activates the transcription of glnAp2 both in vivo and in vitro
Title: FIS activates glnAp2 in Escherichia coli: role of a DNA bend centered at -55, upstream of the transcription start site.
Data suggest that activator must approach the Esigma54 closed complexes from the unbound face of the glnA promoter DNA helix to catalyse open complex formation.
Title: Protein-induced DNA bending clarifies the architectural organization of the sigma54-dependent glnAp2 promoter.
Escherichia coli glutamine synthetase central loop is flexible and functionally passive
Title: The central loop of Escherichia coli glutamine synthetase is flexible and functionally passive.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables glutamine synthetase activity	IBA Inferred from Biological aspect of Ancestor more info
enables glutamine synthetase activity	IDA Inferred from Direct Assay more info	PubMed
enables glutamine synthetase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ligase activity	IEA Inferred from Electronic Annotation more info
enables ligase activity, forming carbon-nitrogen bonds	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within ammonia assimilation cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in glutamine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glutamine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in nitrogen utilization	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within nitrogen utilization	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to radiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	HDA more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	HDA more info	PubMed
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info