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thiF sulfur carrier protein ThiS adenylyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948500, updated on 17-Dec-2024

Summary

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Official Symbol
thiF
Official Full Name
sulfur carrier protein ThiS adenylyltransferase
Primary source
ECOCYC:EG11587
Locus tag
b3992
See related
ASAP:ABE-0013053
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3984; thiA
Summary
The ThiF sequence has been confirmed using MS/MS, however since use of the start codon was forced during cloning, this is not a verification of the start site: the measured mass of the intact protein is 26970.2 indicating that the initial Met is not removed (Kelleher, 1998). [More information is available at EcoGene: EG11587]. The ThiF protein catalyzes the adenylation of the ThiS protein as part of the formation of the thiazole moiety during thiamin biosynthesis . [More information is available at EcoCyc: EG11587].
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Genomic context

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Sequence:
NC_000913.3 (4192821..4193576, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene 2-iminoacetate synthase Neighboring gene 1-deoxy-D-xylulose 5-phosphate:thiol sulfurtransferase Neighboring gene sulfur carrier protein ThiS Neighboring gene thiamine phosphate synthase Neighboring gene phosphomethylpyrimidine synthase Neighboring gene regulator of sigma D

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Structural analysis of Escherichia coli ThiF. Duda DM, et al. J Mol Biol, 2005 Jun 17. PMID 15896804
  2. Biosynthesis of the thiazole moiety of thiamin in Escherichia coli: identification of an acyldisulfide-linked protein--protein conjugate that is functionally analogous to the ubiquitin/E1 complex. Xi J, et al. Proc Natl Acad Sci U S A, 2001 Jul 17. PMID 11438688, Free PMC Article
  3. Structure of the Escherichia coli ThiS-ThiF complex, a key component of the sulfur transfer system in thiamin biosynthesis. Lehmann C, et al. Biochemistry, 2006 Jan 10. PMID 16388576, Free PMC Article
  4. Thiamin biosynthesis in Escherichia coli. Identification of ThiS thiocarboxylate as the immediate sulfur donor in the thiazole formation. Taylor SV, et al. J Biol Chem, 1998 Jun 26. PMID 9632726
  5. The enzymology of sulfur activation during thiamin and biotin biosynthesis. Begley TP, et al. Curr Opin Chem Biol, 1999 Oct. PMID 10508664

See all (17) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. crystal structures of ThiF, alone and in complex with ATP
    Title: Structural analysis of Escherichia coli ThiF.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables AMPylase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotidyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables nucleotidyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables sulfotransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables thiosulfate sulfurtransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ubiquitin-like modifier activating enzyme activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in thiamine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in thiamine diphosphate biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within thiazole biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of adenylyltransferase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of sulfurtransferase complex NAS
Non-traceable Author Statement
more info
 PubMed 

General protein information

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Preferred Names
sulfur carrier protein ThiS adenylyltransferase
NP_418420.4

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4192821..4193576 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418420.4 sulfur carrier protein ThiS adenylyltransferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418420.4

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A1V2G5P4, A0A8S7CSR0
    Conserved Domains (1) summary
    TIGR02356
    Location:8 → 209
    adenyl_thiF; thiazole biosynthesis adenylyltransferase ThiF, E. coli subfamily
Nucleotide Protein
Heading Accession and Version

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