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hinT purine nucleoside phosphoramidase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948549, updated on 17-Dec-2024

Summary

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Official Symbol
hinT
Official Full Name
purine nucleoside phosphoramidase
Primary source
ECOCYC:EG12172
Locus tag
b1103
See related
ASAP:ABE-0003729
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1089; ycfF
Summary
HinT hydrolyzes lysyl-AMP, an activity that may be involved in the regulation of translation or the scavanging of aminoacyl-adenylates (Chou, 2006). hinT is essential for growth under high salt conditions (Chou, 2005). [More information is available at EcoGene: EG12172]. HinT is a purine nucleoside phosphoramidase with a preference for purine nucleoside phosphoramidates. [More information is available at EcoCyc: EG12172].
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Genomic context

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Sequence:
NC_000913.3 (1161885..1162244)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene glucose-specific PTS enzyme IIBC component Neighboring gene ferric coprogen/ferric rhodotorulic acid outer membrane transporter Neighboring gene DUF1425 domain-containing protein YcfL Neighboring gene outer membrane lipoprotein - activator of MrcB activity Neighboring gene thiamine kinase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Probing the impact of the echinT C-terminal domain on structure and catalysis. Bardaweel S, et al. J Mol Biol, 2010 Dec 10. PMID 20934431
  2. 31P NMR and genetic analysis establish hinT as the only Escherchia coli purine nucleoside phosphoramidase and as essential for growth under high salt conditions. Chou TF, et al. J Biol Chem, 2005 Apr 15. PMID 15703176, Free PMC Article
  3. Enzymic cleavage of phosphoramidic acid. SMITH RA, et al. Biochim Biophys Acta, 1959 Jul. PMID 13832126
  4. Synthesis and evaluation of potential inhibitors of human and Escherichia coli histidine triad nucleotide binding proteins. Bardaweel SK, et al. Bioorg Med Chem Lett, 2012 Jan 1. PMID 22104145
  5. Impact of the C-terminal loop of histidine triad nucleotide binding protein1 (Hint1) on substrate specificity. Chou TF, et al. Biochemistry, 2007 Nov 13. PMID 17939685

See all (15) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. evaluation of potential inhibitors of human and Escherichia coli histidine triad nucleotide binding proteins
    Title: Synthesis and evaluation of potential inhibitors of human and Escherichia coli histidine triad nucleotide binding proteins.
  2. The ability of the C-terminal deletion mutants to hydrolyze lysyl-AMP generated by LysU was greatly impaired.
    Title: Probing the impact of the echinT C-terminal domain on structure and catalysis.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables adenosine 5'-monophosphoramidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables adenosine 5'-monophosphoramidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in D-alanine catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_positive_effect D-alanine catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
purine nucleoside phosphoramidase

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1161885..1162244
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415621.3 purine nucleoside phosphoramidase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415621.3

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A192CLP9, A0A1X3I9E4, A0A1X3J458, A0A1X3JIY6, A0A829L9S2, A0AAN3M740, F4SWE1
    Conserved Domains (1) summary
    PRK10687
    Location:1 → 119
    PRK10687; purine nucleoside phosphoramidase; Provisional
Nucleotide Protein
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Gene LinkOut

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