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aphA acid phosphatase/phosphotransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948562, updated on 3-Dec-2024

Summary

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Official Symbol
aphA
Official Full Name
acid phosphatase/phosphotransferase
Primary source
ECOCYC:EG11934
Locus tag
b4055
See related
ASAP:ABE-0013283
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4047; hobH; yjbP
Summary
Mutants arrest cell division after hyperosmoshock and overexpress SeqA; also binds hemi-methylated oriC DNA in vitro. [More information is available at EcoGene: EG11934]. Escherichia coli contains a periplasmic acid phosphatase/phosphotransferase. [More information is available at EcoCyc: EG11934].
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Genomic context

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Sequence:
NC_000913.3 (4269414..4270127)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene tyrosine aminotransferase Neighboring gene uncharacterized protein YjbS Neighboring gene PF01894 family protein YjbQ Neighboring gene PF04237 family protein YjbR

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Structural insights into the catalytic mechanism of the bacterial class B phosphatase AphA belonging to the DDDD superfamily of phosphohydrolases. Leone R, et al. J Mol Biol, 2008 Dec 12. PMID 18845157
  2. Identification of an Escherichia coli periplasmic acid phosphatase containing of a 27 kDa-polypeptide component. Rossolini GM, et al. FEMS Microbiol Lett, 1994 May 1. PMID 8013875
  3. Genetic rearrangements in the tyrB-uvrA region of the enterobacterial chromosome: a potential cause for different class B acid phosphatase regulation in Salmonella enterica and Escherichia coli. Thaller MC, et al. FEMS Microbiol Lett, 1999 Dec 1. PMID 10564784
  4. Expression, purification, crystallization and preliminary X-ray characterization of the class B acid phosphatase (AphA) from Escherichia coli. Forleo C, et al. Acta Crystallogr D Biol Crystallogr, 2003 Jun. PMID 12777773
  5. Biochemical characterization of the class B acid phosphatase (AphA) of Escherichia coli MG1655. Passariello C, et al. Biochim Biophys Acta, 2006 Jan. PMID 16297670

See all (24) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The present work reports the crystal structures of AphA from E. coli in complex with substrate, transition-state, and intermediate analogues.
    Title: Structural insights into the catalytic mechanism of the bacterial class B phosphatase AphA belonging to the DDDD superfamily of phosphohydrolases.
  2. An ushA aphA double knockout mutant was shown to be unable to grow on purine nucleotides as the sole carbon source.
    Title: Complete deficiency of 5'-nucleotidase activity in Escherichia coli leads to loss of growth on purine nucleotides but not of their excretion.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: Hemi-methylated oriC DNA binding activity found in non-specific acid phosphatase.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables L-phosphoserine phosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables acid phosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables acid phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in outer membrane-bounded periplasmic space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in outer membrane-bounded periplasmic space IEA
Inferred from Electronic Annotation
more info
  
located_in periplasmic space IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
acid phosphatase/phosphotransferase
NP_418479.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4269414..4270127
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418479.1 acid phosphatase/phosphotransferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418479.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S0G4R1
    Conserved Domains (1) summary
    PRK11009
    Location:1 → 237
    aphA; class B acid phosphatase
Nucleotide Protein
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