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dut dUTP diphosphatase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948607, updated on 3-Dec-2024

Summary

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Official Symbol
dut
Official Full Name
dUTP diphosphatase
Primary source
ECOCYC:EG10251
Locus tag
b3640
See related
ASAP:ABE-0011899
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
dnaS; ECK3630; sof
Summary
dut is an essential gene. [More information is available at EcoGene: EG10251]. Deoxyuridine triphosphatase (dUTPase) catalyzes the hydrolysis of dUTP, maintaining a low intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. dUTPase is specific for dUTP as a substrate, the active site discriminating between nucleotides with respect to the sugar moiety as well as the pyrimidine base. [More information is available at EcoCyc: EG10251].
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Genomic context

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Sequence:
NC_000913.3 (3813929..3814387)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 50S ribosomal subunit protein L28 Neighboring gene RadC-like JAB domain-containing protein YicR Neighboring gene fused 4'-phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase Neighboring gene nucleoid occlusion factor SlmA Neighboring gene orotate phosphoribosyltransferase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Atomic resolution structure of Escherichia coli dUTPase determined ab initio. González A, et al. Acta Crystallogr D Biol Crystallogr, 2001 Jun. PMID 11375495
  2. Transient accumulation of Okazaki fragments as a result of uracil incorporation into nascent DNA. Tye BK, et al. Proc Natl Acad Sci U S A, 1977 Jan. PMID 319455, Free PMC Article
  3. Overproduction and large-scale preparation of deoxyuridine triphosphate nucleotidohydrolase from Escherichia coli. Hoffmann I, et al. Eur J Biochem, 1987 Apr 1. PMID 3030754
  4. Isolation and characterization of the dut gene of Escherichia coli. II. Restriction enzyme mapping and analysis of polypeptide products. Lundberg LG, et al. Gene, 1983 Apr. PMID 6134654
  5. Crystal structure of the Escherichia coli dUTPase in complex with a substrate analogue (dUDP). Larsson G, et al. Nat Struct Biol, 1996 Jun. PMID 8646539

See all (38) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data conclude that beta-OH of the strictly conserved serine 72 exercises both ground state (GS) destabilization and transition state (TS) stabilization, effects not usually linked to a single residue.
    Title: A double role for a strictly conserved serine: further insights into the dUTPase catalytic mechanism.
  2. Crystal structure of E. coli dUTPase:alpha,beta-methylene-dUDP and E. coli dUTPase:dUDP:Mn complexes were determined
    Title: Methylene substitution at the alpha-beta bridging position within the phosphate chain of dUDP profoundly perturbs ligand accommodation into the dUTPase active site.
  3. influence of the ung, dug, and dut genes in preventing uracil residues from accumulating in E. coli DNA was quantitatively determined
    Title: Quantitative determination of uracil residues in Escherichia coli DNA: Contribution of ung, dug, and dut genes to uracil avoidance.
  4. dUTPase from Drosophila has a similar affinity of binding towards alpha,beta-imino-dUTP as compared to the prokaryotic E. coli enzyme
    Title: Mechanistic studies of dUTPases.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables dUTP diphosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables dUTP diphosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables dUTP diphosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables dUTP diphosphatase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in dUMP biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within dUMP biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in dUMP biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in dUMP biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in dUTP catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within dUTP catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in dUTP catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in dUTP catabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in nucleotide metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within protein homotrimerization IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol HDA PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of protein-containing complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
dUTP diphosphatase
NP_418097.2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3813929..3814387
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418097.2 dUTP diphosphatase [Escherichia coli str. K-12 substr. MG1655]

    Status: PROVISIONAL

    UniProtKB/Swiss-Prot
    B1X974, C4ZXN2
    UniProtKB/TrEMBL
    A0A024LAQ7, A0A236LP18
    Conserved Domains (1) summary
    PRK00601
    Location:2 → 152
    dut; dUTP diphosphatase
Nucleotide Protein
Heading Accession and Version

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