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trmJ tRNA Cm32/Um32 methyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948610, updated on 17-Dec-2024

Summary

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Official Symbol
trmJ
Official Full Name
tRNA Cm32/Um32 methyltransferase
Primary source
ECOCYC:G7327
Locus tag
b2532
See related
ASAP:ABE-0008332
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2529; yfhQ
Summary
TrmJ is the tRNA:Cm32/Um32 methyltransferase which introduces methyl groups at the 2'-O position of C32 and U32 of several tRNAs in vitro. [More information is available at EcoCyc: G7327].
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Genomic context

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Sequence:
NC_000913.3 (2662583..2663323, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene cysteine desulfurase IscS Neighboring gene DNA-binding transcriptional dual regulator IscR Neighboring gene Nus factor SuhB Neighboring gene putative peptidase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. tRNA recognition by a bacterial tRNA Xm32 modification enzyme from the SPOUT methyltransferase superfamily. Liu RJ, et al. Nucleic Acids Res, 2015 Sep 3. PMID 26202969, Free PMC Article
  2. Hyperproduction of recombinant ferredoxins in escherichia coli by coexpression of the ORF1-ORF2-iscS-iscU-iscA-hscB-hs cA-fdx-ORF3 gene cluster. Nakamura M, et al. J Biochem, 1999 Jul. PMID 10393315
  3. Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features. Yamamoto Y, et al. DNA Res, 1997 Apr 28. PMID 9205837
  4. The yfhQ gene of Escherichia coli encodes a tRNA:Cm32/Um32 methyltransferase. Purta E, et al. BMC Mol Biol, 2006 Jul 18. PMID 16848900, Free PMC Article
  5. Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth. Lopez-Campistrous A, et al. Mol Cell Proteomics, 2005 Aug. PMID 15911532

See all (11) citations in PubMed

GeneRIFs: Gene References Into Functions

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables RNA methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in RNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA nucleoside ribose methylation IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within tRNA nucleoside ribose methylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tRNA processing IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
tRNA Cm32/Um32 methyltransferase
NP_417027.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2662583..2663323 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417027.1 tRNA Cm32/Um32 methyltransferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417027.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9APK7
    Conserved Domains (1) summary
    PRK15114
    Location:1 → 244
    PRK15114; tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ; Provisional
Nucleotide Protein
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