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    SAD1 mRNA splicing protein SAD1 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 850555, updated on 9-Dec-2024

    Summary

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    Official Symbol
    SAD1
    Official Full Name
    mRNA splicing protein SAD1
    Primary source
    SGD:S000001901
    Locus tag
    YFR005C
    See related
    AllianceGenome:SGD:S000001901; FungiDB:YFR005C; VEuPathDB:YFR005C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Predicted to enable cysteine-type deubiquitinase activity and zinc ion binding activity. Involved in spliceosomal complex assembly. Located in nucleus. Orthologous to human USP39 (ubiquitin specific peptidase 39). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See SAD1 in Genome Data Viewer
    Location:
    chromosome: VI
    Exon count:
    1
    Sequence:
    Chromosome: VI; NC_001138.5 (154527..155873, complement)

    Chromosome VI - NC_001138.5Genomic Context describing neighboring genes Neighboring gene type 1 protein phosphatase-activating protein YPI1 Neighboring gene proteasome regulatory particle lid subunit RPN11 Neighboring gene putative Xaa-Pro dipeptidase Neighboring gene tRNA-Phe

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001138.5

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre-mRNA splicing. Lygerou Z, et al. Mol Cell Biol, 1999 Mar. PMID 10022888, Free PMC Article
    2. Telomere anchoring at the nuclear periphery requires the budding yeast Sad1-UNC-84 domain protein Mps3. Bupp JM, et al. J Cell Biol, 2007 Dec 3. PMID 18039933, Free PMC Article
    3. The crystal structure of S. cerevisiae Sad1, a catalytically inactive deubiquitinase that is broadly required for pre-mRNA splicing. Hadjivassiliou H, et al. RNA, 2014 May. PMID 24681967, Free PMC Article
    4. The SAD1/RAD53 protein kinase controls multiple checkpoints and DNA damage-induced transcription in yeast. Allen JB, et al. Genes Dev, 1994 Oct 15. PMID 7958905
    5. An Allosteric Network for Spliceosome Activation Revealed by High-Throughput Suppressor Analysis in Saccharomyces cerevisiae. Brow DA. Genetics, 2019 May. PMID 30898770, Free PMC Article

    See all (35) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. we propose that Sad1's ZnF-UBP acts in a ubiquitin-independent capacity to recruit and/or activate Sad1's iUSP domain to interact with the spliceosome
      Title: The crystal structure of S. cerevisiae Sad1, a catalytically inactive deubiquitinase that is broadly required for pre-mRNA splicing.
    2. Sad1 maintains the integrity of the tri-snRNP by counteracting Brr2-mediated dissociation of tri-snRNP.
      Title: Sad1 counteracts Brr2-mediated dissociation of U4/U6.U5 in tri-snRNP homeostasis.
    3. A previously unrecognized function for the Saccharomyces cerevisiae Sad1-UNC-84 domain protein Mps3 in regulating telomere positioning in mitotic cells, is reported.
      Title: Telomere anchoring at the nuclear periphery requires the budding yeast Sad1-UNC-84 domain protein Mps3.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables cysteine-type deubiquitinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in RNA splicing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA splicing, via spliceosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein deubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in spliceosomal complex assembly IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in spliceosomal complex assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in spliceosomal complex assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of spliceosomal complex IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    mRNA splicing protein SAD1
    NP_116660.1
    • Conserved zinc-finger domain protein involved in pre-mRNA splicing; critical for splicing of nearly all intron-containing genes; required for assembly of U4 snRNA into the U4/U6 particle

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001138.5 Reference assembly

      Range
      154527..155873 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001179970.1 → NP_116660.1  TPA: mRNA splicing protein SAD1 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_116660.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VTN5, P43589
      UniProtKB/TrEMBL
      A7A243, G2WDE4, N1P378
      Conserved Domains (1) summary
      cd02669
      Location:30 → 445
      Peptidase_C19M; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P43589.1

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