Grk3 G protein-coupled receptor kinase 3 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Grk3provided by RGD
Official Full Name: G protein-coupled receptor kinase 3provided by RGD
Primary source: RGD:2063
See related: EnsemblRapid:ENSRNOG00000059456 AllianceGenome:RGD:2063
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Adrbk2
Summary: Enables D1 dopamine receptor binding activity; G protein-coupled receptor kinase activity; and beta-adrenergic receptor kinase activity. Involved in several processes, including desensitization of G protein-coupled receptor signaling pathway; inositol phosphate metabolic process; and rhodopsin metabolic process. Located in several cellular components, including Z disc; dendrite; and sperm midpiece. Is active in glutamatergic synapse; postsynaptic density; and presynapse. Biomarker of Parkinson's disease; hypertension; and hypothyroidism. Orthologous to human GRK3 (G protein-coupled receptor kinase 3). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Brain (RPKM 94.3), Spleen (RPKM 77.4) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 12q16

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	12	NC_086030.1 (49285228..49395803)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	12	NC_051347.1 (43624778..43735375)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	12	NC_005111.4 (49626871..49750389)

Chromosome 12 - NC_086030.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Our findings reveal a novel mechanism of GRK3 control of the dopamine receptor signaling and the role of Gq in LID.
Title: GRK3 suppresses L-DOPA-induced dyskinesia in the rat model of Parkinson's disease via its RGS homology domain.
the loss of GRK3-mediated regulation of CXCL12/CXCR4 signaling contributes to some, but not all, of the complete WHIM phenotype and that GRK3 inhibition may be beneficial in the treatment of inflammatory arthritis.
Title: G protein-coupled receptor kinase-3-deficient mice exhibit WHIM syndrome features and attenuated inflammatory responses.
We suggest that reduced stress resilience after TSS may be related to oxidative stress, depletion of GRK3 and impaired regulation of alpha(2A)-AR and CRF(1)-R in LC.
Title: Differential effects of inescapable stress on locus coeruleus GRK3, alpha2-adrenoceptor and CRF1 receptor levels in learned helpless and non-helpless rats: a potential link to stress resilience.
CRF selectively up-regulates GRK3 expression via an ERK1/2-mediated mechanism accompanied by the activation of Sp-1 and Ap-2 transcription factors.
Title: Activation of the CRF(1) receptor causes ERK1/2 mediated increase in GRK3 expression in CATH.a cells.
Substrate specificity of Grk3 at cardiac myocyte receptors provides basis for distinct role in regulation of myocardial function.
Title: Substrate specificities of g protein-coupled receptor kinase-2 and -3 at cardiac myocyte receptors provide basis for distinct roles in regulation of myocardial function.
Gs subunits and beta2AR utilize different internalization mechanisms.
Title: Live cell imaging of Gs and the beta2-adrenergic receptor demonstrates that both alphas and beta1gamma7 internalize upon stimulation and exhibit similar trafficking patterns that differ from that of the beta2-adrenergic receptor.
G-protein-coupled receptor kinase (GRK3) and beta-arrestin-2 play a role in Xenopus oocyte maturation.
Title: A G protein-coupled receptor kinase induces Xenopus oocyte maturation.

Submit: New GeneRIF Correction

General gene information

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Markers

UniSTS:235264 (e-PCR)
- UniSTS:235264

AU046729 (e-PCR)
- UniSTS:111187

BE119364 (e-PCR)
- UniSTS:249173

AU046882 (e-PCR)
- UniSTS:111047

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables D1 dopamine receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables G protein-coupled receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables G protein-coupled receptor kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables G protein-coupled receptor kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables G protein-coupled receptor kinase activity	IEA Inferred from Electronic Annotation more info
enables G protein-coupled receptor kinase activity	ISO Inferred from Sequence Orthology more info
enables beta-adrenergic receptor kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables beta-adrenergic receptor kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in G protein-coupled receptor signaling pathway	IEP Inferred from Expression Pattern more info	PubMed
involved_in desensitization of G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in desensitization of G protein-coupled receptor signaling pathway	IC Inferred by Curator more info	PubMed
involved_in desensitization of G protein-coupled receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in desensitization of G protein-coupled receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in inositol phosphate metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of G protein-coupled receptor signaling pathway	IC Inferred by Curator more info	PubMed
involved_in positive regulation of protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in receptor internalization	IEA Inferred from Electronic Annotation more info
involved_in receptor internalization	ISO Inferred from Sequence Orthology more info
involved_in rhodopsin metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in Z disc	IDA Inferred from Direct Assay more info	PubMed
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in dendrite terminus	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic shaft	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic spine	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynapse	IDA Inferred from Direct Assay more info	PubMed
located_in presynapse	IEA Inferred from Electronic Annotation more info
located_in sperm midpiece	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: G protein-coupled receptor kinase 3

Names: adrenergic receptor kinase, beta 2 (G-protein-linked receptor kinase); adrenergic, beta, receptor kinase 2; beta ARK2; beta-ARK-2; beta-adrenergic receptor kinase 2

NP_037029.2

EC 2.7.11.15

XP_038945037.1

EC 2.7.11.15

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_012897.4 → NP_037029.2 G protein-coupled receptor kinase 3

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000012

UniProtKB/Swiss-Prot

P26819

UniProtKB/TrEMBL

A0A0G2K145

Related

ENSRNOP00000071696.2, ENSRNOT00000082322.3

Conserved Domains (3) summary

cd08747
Location:30 → 186

RGS_GRK2_GRK3; Regulator of G protein signaling domain (RGS) found in G protein-coupled receptor kinase 2 (GRK2) and G protein-coupled receptor kinase 3 (GRK3)

cd01240
Location:553 → 670

PH_GRK2_subgroup; G Protein-Coupled Receptor Kinase 2 subgroup pleckstrin homology (PH) domain

cl21453
Location:185 → 530

PKc_like; Protein Kinases, catalytic domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086030.1 Reference GRCr8

Range

49285228..49395803

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039089109.2 → XP_038945037.1 G protein-coupled receptor kinase 3 isoform X1

UniProtKB/Swiss-Prot

P26819

Conserved Domains (3) summary

cd08747
Location:30 → 186

RGS_GRK2_GRK3; Regulator of G protein signaling domain (RGS) found in G protein-coupled receptor kinase 2 (GRK2) and G protein-coupled receptor kinase 3 (GRK3)

cl17171
Location:553 → 635

PH-like; Pleckstrin homology-like domain

cl21453
Location:185 → 530

PKc_like; Protein Kinases, catalytic domain