Dhx33 DEAH-box helicase 33 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dhx33provided by MGI
Official Full Name: DEAH-box helicase 33provided by MGI
Primary source: MGI:MGI:2445102
See related: Ensembl:ENSMUSG00000040620 AllianceGenome:MGI:2445102
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ddx33; 3110057P17Rik; 9430096J02Rik
Summary: Enables DNA-binding transcription factor binding activity; double-stranded RNA binding activity; and rDNA binding activity. Involved in positive regulation of MAPK cascade; regulation of gene expression; and translational initiation. Located in nucleolus and nucleoplasm. Orthologous to human DHX33 (DEAH-box helicase 33). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in limb E14.5 (RPKM 8.9), ovary adult (RPKM 7.3) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 B4; 11 43.21 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (70874917..70895258, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (70984091..71004533, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dhx33 promotes B-cell growth and proliferation by controlling activation-induced rRNA upregulation.
Title: Dhx33 promotes B-cell growth and proliferation by controlling activation-induced rRNA upregulation.
Function of DHX33 in promoting Warburg effect via regulation of glycolytic genes.
Title: Function of DHX33 in promoting Warburg effect via regulation of glycolytic genes.
Targeting RNA helicase DHX33 blocks Ras-driven lung tumorigenesis in vivo.
Title: Targeting RNA helicase DHX33 blocks Ras-driven lung tumorigenesis in vivo.
DHX33 hydrolyzes ATP in the presence of DNA/RNA duplexes.DHX33 protein possesses unwinding activity toward DNA and RNA duplexes.
Title: Recombinant DHX33 Protein Possesses Dual DNA/RNA Helicase Activity.
Usp36 is essential for cell and organism viability because of its role in ribosomal RNA processing and protein synthesis, which is mediated, at least in part, by regulating DHX33 stability.
Title: Loss of the deubiquitinase USP36 destabilizes the RNA helicase DHX33 and causes preimplantation lethality in mice.
DHX33 doublet is due to alternative translation initiation by two in-frame initiation codons. DHX33 translation initiation from either AUG codon happens at equal efficiency. Short DHX33 protein has similar cellular location and functions with full-length DHX33. Leaky scanning normally occurs in DHX33 mRNA translation, which may serve as a safeguard mechanism to ensure optimal DHX33 translation efficiency.
Title: Alternative translation initiation from two in-frame start codons in DHX33 gene.
results directly implicate DHX33 as a crucial player in establishing rRNA synthesis rates in the face of Ras(V12) or ARF signals, adjusting ribosome biogenesis to match the appropriate growth or antigrowth signals
Title: P19ARF and RasV¹² offer opposing regulation of DHX33 translation to dictate tumor cell fate.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3) 1 citation
Targeted (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH69933 (e-PCR)
- UniSTS:78643

NoName (e-PCR)
- UniSTS:236627

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA helicase activity	IEA Inferred from Electronic Annotation more info
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables double-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables double-stranded RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded RNA binding	ISO Inferred from Sequence Orthology more info
enables double-stranded RNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	ISO Inferred from Sequence Orthology more info
enables mRNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables rDNA binding	IDA Inferred from Direct Assay more info	PubMed
enables rDNA binding	ISO Inferred from Sequence Orthology more info
enables ribosomal large subunit binding	IEA Inferred from Electronic Annotation more info
enables ribosomal large subunit binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of NF-kappaB transcription factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of NLRP3 inflammasome complex assembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of NLRP3 inflammasome complex assembly	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of transcription by RNA polymerase I	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription by RNA polymerase I	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase I	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of type I interferon production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in translational initiation	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of NLRP3 inflammasome complex	ISO Inferred from Sequence Orthology more info
part_of NLRP3 inflammasome complex	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
is_active_in nucleolus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: ATP-dependent RNA helicase DHX33

Names: DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 33; DEAH (Asp-Glu-Ala-His) box polypeptide 33; DEAH box protein 33; putative ATP-dependent RNA helicase DHX33

NP_848144.3

EC 3.6.4.13

XP_036012469.1

EC 3.6.4.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_178367.4 → NP_848144.3 ATP-dependent RNA helicase DHX33

See identical proteins and their annotated locations for NP_848144.3

Status: VALIDATED

Source sequence(s)

AK087592, AL596136, BC052172

Consensus CDS

CCDS24971.1

UniProtKB/Swiss-Prot

Q80VY9, Q8BS50

Related

ENSMUSP00000104167.2, ENSMUST00000108527.8

Conserved Domains (6) summary

smart00487
Location:49 → 252

DEXDc; DEAD-like helicases superfamily

smart00847
Location:469 → 554

HA2; Helicase associated domain (HA2) Add an annotation

cd00046
Location:83 → 225

DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.

pfam00271
Location:282 → 399

Helicase_C; Helicase conserved C-terminal domain

pfam07717
Location:588 → 688

OB_NTP_bind; Oligonucleotide/oligosaccharide-binding (OB)-fold

pfam11422
Location:409 → 500

IBP39; Initiator binding protein 39 kDa

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

70874917..70895258 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036156576.1 → XP_036012469.1 ATP-dependent RNA helicase DHX33 isoform X1

Conserved Domains (1) summary

COG1643
Location:1 → 523

HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]