RAD2 ssDNA endodeoxyribonuclease RAD2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RAD2
Official Full Name: ssDNA endodeoxyribonuclease RAD2
Primary source: SGD:S000003490
Locus tag: YGR258C
See related: AllianceGenome:SGD:S000003490; FungiDB:YGR258C; VEuPathDB:YGR258C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables single-stranded DNA endodeoxyribonuclease activity. Involved in nucleotide-excision repair and transcription by RNA polymerase II. Part of nucleotide-excision repair factor 3 complex. Used to study Cockayne syndrome and xeroderma pigmentosum. Human ortholog(s) of this gene implicated in several diseases, including cerebrooculofacioskeletal syndrome 3; melanoma; respiratory system cancer (multiple); stomach cancer; and xeroderma pigmentosum group G. Orthologous to human ERCC5 (ERCC excision repair 5, endonuclease). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RAD2 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (1007671..1010766, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Interplay of the Tfb1 pleckstrin homology domain with Rad2 and Rad4 in transcription coupled and global genomic nucleotide excision repair.
Title: Interplay of the Tfb1 pleckstrin homology domain with Rad2 and Rad4 in transcription coupled and global genomic nucleotide excision repair.
In a mutant affected in Pol II phosphorylation leading to Mediator stabilization on core promoters, Rad2 genome-wide occupancy shifts towards core promoters following that of Mediator, but decreases on transcribed regions together with Pol II.
Title: Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin.
Rad2 and rad27 overexpression increases genomic instability in yeast. Rad2 and Rad27 causes DNA breaks that are processed by different repair functions.
Title: Differential effect of the overexpression of Rad2/XPG family endonucleases on genome integrity in yeast and human cells.
THe data suggest that in contrast to related enzymes (FEN1 and EXO1), the Rad2 active site may be more accessible, which would create an exit route for DNA substrates without a free 5' end.
Title: Crystal structure of the catalytic core of Rad2: insights into the mechanism of substrate binding.
Study shows that Rad2p interacts with PCNA through its proliferating cell nuclear antigen-binding domain (PCNA-BD) and the PCNA-BD of Rad2p plays a role in UV-induced mutagenesis.
Title: The PCNA binding domain of Rad2p plays a role in mutagenesis by modulating the cell cycle in response to DNA damage.
Structure determination of a Rad2-Tfb1PH complex indicates that Rad2 binds to TFIIH using a similar motif as TFIIEalpha uses to bind TFIIH in the pre-initiation complex.
Title: Structural and functional characterization of interactions involving the Tfb1 subunit of TFIIH and the NER factor Rad2.
These observations suggest that RAD2 has a function in coordinating cell cycle regulation and damaged DNA repair.
Title: Mitotic catastrophe induced by overexpression of budding yeast Rad2p.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA nuclease activity	IEA Inferred from Electronic Annotation more info
enables endonuclease activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded DNA binding	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA endodeoxyribonuclease activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in nucleotide-excision repair	IDA Inferred from Direct Assay more info	PubMed
involved_in nucleotide-excision repair	IEA Inferred from Electronic Annotation more info
involved_in nucleotide-excision repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of nucleotide-excision repair factor 3 complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ssDNA endodeoxyribonuclease RAD2

NP_011774.1

Single-stranded DNA endonuclease; cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

1007671..1010766 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181387.1 → NP_011774.1 TPA: ssDNA endodeoxyribonuclease RAD2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011774.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VV38, E9P8X9, P07276

UniProtKB/TrEMBL

C7GNH3, N1P444

Conserved Domains (1) summary

TIGR00600
Location:1 → 1030

rad2; DNA excision repair protein (rad2)