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    hsdS type I restriction enzyme EcoKI specificity subunit [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948867, updated on 3-Dec-2024

    Summary

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    Official Symbol
    hsdS
    Official Full Name
    type I restriction enzyme EcoKI specificity subunit
    Primary source
    ECOCYC:EG10460
    Locus tag
    b4348
    See related
    ASAP:ABE-0014258
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4338; hsp; hss; rm
    Summary
    Substitution of Trp212 to Arg alters the protein-protein interaction with HsdM, in turn effecting the DNA binding of the molecule; DNA binding in the mutant is restored by HsdR binding. [More information is available at EcoGene: EG10460]. HsdS is the specificity-determining component of the EcoKI restriction-modification system . [More information is available at EcoCyc: EG10460].
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    Genomic context

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    Sequence:
    NC_000913.3 (4580068..4581462, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene Type IV methyl-directed restriction enzyme EcoKMcrBC subunit Neighboring gene 5-methylcytosine-specific restriction enzyme subunit McrB;McrBS Neighboring gene nucleoid-associated protein SymE Neighboring gene ncRNA Neighboring gene type I restriction enzyme EcoKI methylase subunit Neighboring gene type I restriction enzyme EcoKI endonuclease subunit Neighboring gene Type IV methyl-directed restriction enzyme EcoKMrr

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Tetra-amino-acid tandem repeats are involved in HsdS complementation in type IC restriction-modification systems. Adamczyk-Popławska M, et al. Microbiology (Reading), 2003 Nov. PMID 14600243
    2. Sequence diversity among related genes for recognition of specific targets in DNA molecules. Gough JA, et al. J Mol Biol, 1983 May 5. PMID 6304321
    3. Structural homologies among type I restriction-modification systems. Murray NE, et al. EMBO J, 1982. PMID 6329689, Free PMC Article
    4. The in vitro assembly of the EcoKI type I DNA restriction/modification enzyme and its in vivo implications. Dryden DT, et al. Biochemistry, 1997 Feb 4. PMID 9033396
    5. Purification and biochemical characterisation of the EcoR124 type I modification methylase. Taylor I, et al. Nucleic Acids Res, 1992 Jan 25. PMID 1741244, Free PMC Article

    See all (22) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA restriction-modification system IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA restriction-modification system IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of type I site-specific deoxyribonuclease complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    type I restriction enzyme EcoKI specificity subunit
    NP_418768.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4580068..4581462 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418768.1 type I restriction enzyme EcoKI specificity subunit [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418768.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2W6P716
      Conserved Domains (1) summary
      PRK09737
      Location:1 → 464
      PRK09737; type I restriction-modification system specificity subunit
    Nucleotide Protein
    Heading Accession and Version

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