CEG1 mRNA guanylyltransferase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: CEG1
Official Full Name: mRNA guanylyltransferase
Primary source: SGD:S000003098
Locus tag: YGL130W
See related: AllianceGenome:SGD:S000003098; FungiDB:YGL130W; VEuPathDB:YGL130W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables mRNA guanylyltransferase activity. Involved in 7-methylguanosine mRNA capping; positive regulation of transcription by RNA polymerase II; and tRNA processing. Located in nucleus. Part of mRNA capping enzyme complex. Orthologous to human RNGTT (RNA guanylyltransferase and 5'-phosphatase). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See CEG1 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (266145..267524)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ceg1 depletion reveals mechanisms governing degradation of non-capped RNAs in Saccharomyces cerevisiae.
Title: Ceg1 depletion reveals mechanisms governing degradation of non-capped RNAs in Saccharomyces cerevisiae.
analysis of the role of the Ceg1-carboxy terminal domain interaction in mRNA transcription
Title: The mRNA capping enzyme of Saccharomyces cerevisiae has dual specificity to interact with CTD of RNA Polymerase II.
The results indicate that the Cet1p-Ceg1p interaction is important not only for the activation of Ceg1p, but also for nuclear import of the complex.
Title: The essential role for the RNA triphosphatase Cet1p in nuclear import of the mRNA capping enzyme Cet1p-Ceg1p complex of Saccharomyces cerevisiae.
Data show that the early Pol II elongation factor Spt5 contributes to stable recruitment of the mRNA capping enzymes Cet1, Ceg1, and Abd1 in the initiation-elongation transition of RNA polymerase II.
Title: Cap completion and C-terminal repeat domain kinase recruitment underlie the initiation-elongation transition of RNA polymerase II.
A crystal structure of Cet1-Ceg1 revealed a 176 kDa heterotetrameric complex composed of one Cet1 homodimer that associates with two Ceg1 molecules via interactions between the Ceg1 oligonucleotide binding domain and an extended Cet1 amino acid motif.
Title: Structure of the Saccharomyces cerevisiae Cet1-Ceg1 mRNA capping apparatus.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables mRNA guanylyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA guanylyltransferase activity	IEA Inferred from Electronic Annotation more info
enables mRNA guanylyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables nucleotidyltransferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in 7-methylguanosine mRNA capping	IBA Inferred from Biological aspect of Ancestor more info
involved_in 7-methylguanosine mRNA capping	IEA Inferred from Electronic Annotation more info
involved_in 7-methylguanosine mRNA capping	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleobase-containing compound metabolic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of mRNA capping enzyme complex	IEA Inferred from Electronic Annotation more info
part_of mRNA capping enzyme complex	IGI Inferred from Genetic Interaction more info	PubMed
part_of mRNA capping enzyme complex	IPI Inferred from Physical Interaction more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: mRNA guanylyltransferase

NP_011385.3

EC 2.7.7.50

Guanylyltransferase involved in mRNA 5' capping; subunit of mRNA capping enzyme, which is a heterotetramer composed of two molecules of Ceg1p and a homodimer of Cet1p, the mRNA 5'-triphosphatase subunit; nuclear import of Ceg1p requires interaction with Cet1p; mammalian capping enzyme is a single bifunctional polypeptide; human homolog RNGTT can complement yeast ceg1 null mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

266145..267524

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180995.3 → NP_011385.3 TPA: mRNA guanylyltransferase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011385.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VU18, Q01159, Q9URD1

UniProtKB/TrEMBL

A6ZU52, B3LHI9, B5VIJ9, C7GX64, C8Z8C0, G2WDX2, N1P3E0

Conserved Domains (1) summary

COG5226
Location:1 → 449

CEG1; mRNA capping enzyme, guanylyltransferase (alpha) subunit [RNA processing and modification]