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    EST3 telomerase subunit EST3 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 854806, updated on 9-Dec-2024

    Summary

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    Official Symbol
    EST3
    Official Full Name
    telomerase subunit EST3
    Primary source
    SGD:S000006432
    Locus tag
    YIL009C-A
    See related
    AllianceGenome:SGD:S000006432; FungiDB:YIL009C-A; VEuPathDB:YIL009C-A
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables DNA/RNA helicase activity; GTPase activity; and telomeric DNA binding activity. Involved in telomere maintenance via telomerase. Located in nucleus. Part of telomerase holoenzyme complex. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See EST3 in Genome Data Viewer
    Location:
    chromosome: IX
    Exon count:
    1
    Sequence:
    Chromosome: IX; NC_001141.2 (335666..336212, complement)

    Chromosome IX - NC_001141.2Genomic Context describing neighboring genes Neighboring gene Tir3p Neighboring gene thioredoxin peroxidase DOT5 Neighboring gene tRNA-Asp Neighboring gene long-chain fatty acid-CoA ligase FAA3

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001141.2

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Yeast telomerase protein Est3 is a novel type of GTPase. Shubernetskaya O, et al. Biochimie, 2011 Feb. PMID 20884318
    2. Programmed translational frameshifting in a gene required for yeast telomere replication. Morris DK, et al. Curr Biol, 1997 Dec 1. PMID 9382847
    3. In vitro dimerization of telomerase protein Est3p is stimulated by magnesium cations. Malyavko AG, et al. Dokl Biochem Biophys, 2010 Jul-Aug. PMID 20714845
    4. Dissecting protein function: an efficient protocol for identifying separation-of-function mutations that encode structurally stable proteins. Lubin JW, et al. Genetics, 2013 Mar. PMID 23307900, Free PMC Article
    5. Isolation of active yeast telomerase protein Est3p and investigation of its dimerization in vitro. Sharanov YS, et al. Biochemistry (Mosc), 2007 Jul. PMID 17680761

    See all (66) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Est3 adopts an overall protein fold which is structurally similar to that adopted by the shelterin component TPP1
      Title: Structure of Est3 reveals a bimodal surface with differential roles in telomere replication.
    2. Stimulation of yeast telomerase activity by the ever shorter telomere 3 (Est3) subunit is dependent on direct interaction with the catalytic protein Est2.
      Title: Stimulation of yeast telomerase activity by the ever shorter telomere 3 (Est3) subunit is dependent on direct interaction with the catalytic protein Est2.
    3. Dimerization of Est3p contributes to telomere maintenance.
      Title: Saccharomyces cerevisiae Est3p dimerizes in vitro and dimerization contributes to efficient telomere replication in vivo.
    4. An mRNA sequence from the EST3 gene stimulates programmed +1 translational frameshifting.
      Title: An mRNA sequence derived from the yeast EST3 gene stimulates programmed +1 translational frameshifting.
    5. Est3p binds both DNA and RNA oligonucleotides containing telomere repeat sequences and stimulates dissociation of RNA/DNA heteroduplexes which is important in telomerase function
      Title: Saccharomyces cerevisiae telomerase subunit Est3p binds DNA and RNA and stimulates unwinding of RNA/DNA heteroduplexes.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables DNA/RNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTPase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTPase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables telomeric DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables telomeric DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in telomere maintenance via telomerase IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in telomere maintenance via telomerase IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in telomere maintenance via telomerase IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in viral translational frameshifting IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in chromosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in chromosome, telomeric region IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol HDA PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of telomerase holoenzyme complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of telomerase holoenzyme complex IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    telomerase subunit EST3
    NP_012256.1
    • Component of the telomerase holoenzyme; involved in telomere replication; synthesis of the full-length protein results from a programmed +1 ribosomal frameshift

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001141.2 Reference assembly

      Range
      335666..336212 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001184306.2 → NP_012256.1  TPA: telomerase subunit EST3 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_012256.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VVS0, Q03096
      UniProtKB/TrEMBL
      A6ZVQ8, G2WGD6
      Conserved Domains (1) summary
      pfam10341
      Location:20 → 138
      TPP1; Shelterin complex subunit, TPP1/ACD
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q03096.2

    Gene LinkOut

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