Pcyt1a phosphate cytidylyltransferase 1, choline, alpha isoform [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pcyt1aprovided by MGI
Official Full Name: phosphate cytidylyltransferase 1, choline, alpha isoformprovided by MGI
Primary source: MGI:MGI:88557
See related: Ensembl:ENSMUSG00000005615 AllianceGenome:MGI:88557
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ctpct; CTalpha; Cctalpha; Cttalpha
Summary: Enables choline-phosphate cytidylyltransferase activity. Involved in B cell proliferation; CDP-choline pathway; and isotype switching. Located in endoplasmic reticulum membrane and glycogen granule. Is expressed in several structures, including alimentary system; central nervous system; ear; genitourinary system; and integumental system. Human ortholog(s) of this gene implicated in congenital generalized lipodystrophy and spondylometaphyseal dysplasia with cone-rod dystrophy. Orthologous to human PCYT1A (phosphate cytidylyltransferase 1A, choline). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in small intestine adult (RPKM 19.0), heart adult (RPKM 14.8) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 16 B3; 16 22.69 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	16	NC_000082.7 (32249739..32293883)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	16	NC_000082.6 (32430921..32475065)

Chromosome 16 - NC_000082.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PCYT1A deficiency disturbs fatty acid metabolism and induces ferroptosis in the mouse retina.
Title: PCYT1A deficiency disturbs fatty acid metabolism and induces ferroptosis in the mouse retina.
Intestinal Phospholipid Disequilibrium Initiates an ER Stress Response That Drives Goblet Cell Necroptosis and Spontaneous Colitis in Mice.
Title: Intestinal Phospholipid Disequilibrium Initiates an ER Stress Response That Drives Goblet Cell Necroptosis and Spontaneous Colitis in Mice.
The findings reported herein indicate that palmitate-induced cisternal endoplasmic reticulum expansion is dependent on the activation of XBP-1/CCTalpha-mediated phospholipid accumulation in RAW 264.7 cells.
Title: Palmitate induces cisternal ER expansion via the activation of XBP-1/CCTα-mediated phospholipid accumulation in RAW 264.7 cells.
Data suggest that CTalpha is an important factor in hepatocyte proliferation in vitro; however, liver regeneration, DNA synthesis, and phosphatidylcholine biosynthesis after partial hepatectomy are not impaired in CTalpha(-/-) knockout mice.
Title: Impaired phosphatidylcholine biosynthesis does not attenuate liver regeneration after 70% partial hepatectomy in hepatic CTP:phosphocholine cytidylyltransferase-α deficient mice.
CaMKI vies with CRM1/exportin 1 for access to a nuclear export signal, and assembly of a CaMKI-14-3-3 zeta-CCTalpha complex is a key effector mechanism that drives nuclear CCTalpha translocation.
Title: Calcium-calmodulin kinase I cooperatively regulates nucleocytoplasmic shuttling of CCTα by accessing a nuclear export signal.
This study illustrates, for the first time, the connection between the acute accumulation of farnesylated prelamin A and involvement of CCT-alpha in generating an nucleoplasmic reticulum.
Title: The induction of a nucleoplasmic reticulum by prelamin A accumulation requires CTP:phosphocholine cytidylyltransferase-α.
Enforced expression of either CK(alpha) or CCTalpha increased the rate of synthesis and the amount of PtdCho, and these cells initiated neuronal differentiation.
Title: Phosphatidylcholine biosynthesis during neuronal differentiation and its role in cell fate determination.
14-3-3zeta controls CCTalpha nuclear import in response to calcium signals, thereby regulating mammalian phospholipid synthesis.
Title: 14-3-3zeta escorts CCTalpha for calcium-activated nuclear import in lung epithelia.
CCTalpha is monoubiquitinated at a molecular site (K(57)) juxtaposed near its nuclear localization signal, resulting in disruption of its interaction with importin-alpha, nuclear exclusion, and subsequent degradation within the lysosome.
Title: Masking of a nuclear signal motif by monoubiquitination leads to mislocalization and degradation of the regulatory enzyme cytidylyltransferase.
CTP:phosphocholine cytidylyltransferase alpha is required for B-cell proliferation and class switch recombination.
Title: CTP:phosphocholine cytidylyltransferase alpha is required for B-cell proliferation and class switch recombination.

Submit: New GeneRIF Correction See all GeneRIFs (25)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Gene trapped (1)
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

STS-L28957 (e-PCR)
- UniSTS:78865

Pcyt1a (e-PCR), detects polymorphism
- UniSTS:470561

NoName (e-PCR)
- UniSTS:237486

Pcyt1a (e-PCR), detects polymorphism
- UniSTS:259504

Pcyt1a (e-PCR), detects polymorphism
- UniSTS:259505

NoName (e-PCR)
- UniSTS:237493

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables calmodulin binding	ISO Inferred from Sequence Orthology more info
enables choline-phosphate cytidylyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables choline-phosphate cytidylyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables choline-phosphate cytidylyltransferase activity	ISO Inferred from Sequence Orthology more info
enables choline-phosphate cytidylyltransferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables lipid binding	ISO Inferred from Sequence Orthology more info
enables molecular function inhibitor activity	IEA Inferred from Electronic Annotation more info
enables molecular function inhibitor activity	ISO Inferred from Sequence Orthology more info
enables phosphatidylcholine binding	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylcholine binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylcholine binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in B cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in CDP-choline pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in CDP-choline pathway	ISO Inferred from Sequence Orthology more info
involved_in CDP-choline pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in isotype switching	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within phosphatidylcholine biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphatidylcholine biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within phosphatidylcholine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphatidylcholine biosynthetic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytosol	IEA Inferred from Electronic Annotation more info
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in glycogen granule	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear envelope	IEA Inferred from Electronic Annotation more info
located_in nuclear envelope	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: choline-phosphate cytidylyltransferase A

Names: CCT A; CCT-alpha; CT A; CTP phosphocholine cytidylyl transferase; CTP:phosphocholine cytidylyltransferase A; CTP:phosphocholine cytidylyltransferase alpha; phosphorylcholine transferase A

NP_001156631.1

EC 2.7.7.15

NP_001156632.1

EC 2.7.7.15

NP_034111.1

EC 2.7.7.15

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001163159.1 → NP_001156631.1 choline-phosphate cytidylyltransferase A

See identical proteins and their annotated locations for NP_001156631.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript. All three variants encode the same protein.

Source sequence(s)

AC087556, AK076050

Consensus CDS

CCDS28120.1

UniProtKB/Swiss-Prot

P49586, Q542W4

Related

ENSMUSP00000130056.2, ENSMUST00000104893.10

Conserved Domains (1) summary

cd02174
Location:75 → 224

CCT; CTP:phosphocholine cytidylyltransferase
NM_001163160.1 → NP_001156632.1 choline-phosphate cytidylyltransferase A

See identical proteins and their annotated locations for NP_001156632.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All three variants encode the same protein.

Source sequence(s)

AC087556, AK076050

Consensus CDS

CCDS28120.1

UniProtKB/Swiss-Prot

P49586, Q542W4

Related

ENSMUSP00000110793.2, ENSMUST00000115140.2

Conserved Domains (1) summary

cd02174
Location:75 → 224

CCT; CTP:phosphocholine cytidylyltransferase
NM_009981.4 → NP_034111.1 choline-phosphate cytidylyltransferase A

See identical proteins and their annotated locations for NP_034111.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All three variants encode the same protein.

Source sequence(s)

AC087556, AK076050, BB642590

Consensus CDS

CCDS28120.1

UniProtKB/Swiss-Prot

P49586, Q542W4

Related

ENSMUSP00000078721.5, ENSMUST00000079791.11

Conserved Domains (1) summary

cd02174
Location:75 → 224

CCT; CTP:phosphocholine cytidylyltransferase