ATG16 Atg16p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ATG16
Official Full Name: Atg16p
Primary source: SGD:S000004769
Locus tag: YMR159C
See related: AllianceGenome:SGD:S000004769; FungiDB:YMR159C; VEuPathDB:YMR159C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: APG15; APG16; CVT11; SAP18
Summary: Enables protein-macromolecule adaptor activity. Contributes to Atg8-family ligase activity. Involved in several processes, including C-terminal protein lipidation; cytoplasm to vacuole targeting by the Cvt pathway; and macroautophagy. Located in phagophore; phagophore assembly site; and vacuole-isolation membrane contact site. Part of Atg12-Atg5-Atg16 complex. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See ATG16 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (574476..574928, complement)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Membrane Binding and Homodimerization of Atg16 Via Two Distinct Protein Regions is Essential for Autophagy in Yeast.
Title: Membrane Binding and Homodimerization of Atg16 Via Two Distinct Protein Regions is Essential for Autophagy in Yeast.
Here, the authors discover a novel mechanism for the PAS targeting of Atg12-Atg5-Atg16, which is mediated by the interaction of Atg12 with the Atg1 kinase complex that serves as a scaffold for pre-autophagosomal structure organization.
Title: Two distinct mechanisms target the autophagy-related E3 complex to the pre-autophagosomal structure.
Atg1, Atg8 and the Atg16-Atg12-Atg5 complex were present at both the VICS and the cup-shaped IM.
Title: Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae.
Molecular functions of the Atg12-Atg5-Atg16 complex in autophagy probed by X-ray crystallography.
Title: Structure of the Atg12-Atg5 conjugate reveals a platform for stimulating Atg8-PE conjugation.
It was shown that the yeast Atg5-Atg12/Atg16 complex directly binds membranes. Membrane binding is mediated by Atg5, inhibited by Atg12 and activated by Atg16.
Title: Mechanism and functions of membrane binding by the Atg5-Atg12/Atg16 complex during autophagosome formation.
Evolutionary conserved surface residues clustered at the C-terminal half of Atg16 CCD were shown to be crucial for autophagy.
Title: Dimeric coiled-coil structure of Saccharomyces cerevisiae Atg16 and its functional significance in autophagy.
The coiled-coil domain of Atg16 was expressed, purified and crystallized, and described at 2.5 angstrom resolution.
Title: Crystallization of the coiled-coil domain of Atg16 essential for autophagy.
F154 plays a critical role in the assembly of a functional Atg12-Atg5.Atg16 complex that is requisite for autophagosome formation.
Title: Structure-function relationship of Atg12, a ubiquitin-like modifier essential for autophagy.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to Atg8-family ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein-macromolecule adaptor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in C-terminal protein lipidation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in autophagosome organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in autophagy	IEA Inferred from Electronic Annotation more info
involved_in autophagy of mitochondrion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cytoplasm to vacuole targeting by the Cvt pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in macroautophagy	IDA Inferred from Direct Assay more info	PubMed
involved_in nucleophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in piecemeal microautophagy of the nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of Atg12-Atg5-Atg16 complex	IDA Inferred from Direct Assay more info	PubMed
part_of Atg12-Atg5-Atg16 complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytosol	HDA more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed
located_in phagophore	IDA Inferred from Direct Assay more info	PubMed
located_in phagophore assembly site	IDA Inferred from Direct Assay more info	PubMed
located_in phagophore assembly site membrane	IEA Inferred from Electronic Annotation more info
located_in vacuole-isolation membrane contact site	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Atg16p

NP_013882.1

Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which binds to membranes and localizes to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress