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    Nox4 NADPH oxidase 4 [ Mus musculus (house mouse) ]

    Gene ID: 50490, updated on 9-Dec-2024

    Summary

    Official Symbol
    Nox4provided by MGI
    Official Full Name
    NADPH oxidase 4provided by MGI
    Primary source
    MGI:MGI:1354184
    See related
    Ensembl:ENSMUSG00000030562 AllianceGenome:MGI:1354184
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Summary
    Enables protein tyrosine kinase binding activity and superoxide-generating NAD(P)H oxidase activity. Involved in several processes, including positive regulation of intracellular signal transduction; positive regulation of macromolecule metabolic process; and superoxide anion generation. Acts upstream of or within bone resorption and cardiac muscle cell differentiation. Located in cell periphery; mitochondrion; and perinuclear region of cytoplasm. Is expressed in embryo; heart; metanephros; renal cortex; and ventricle compact myocardium. Orthologous to human NOX4 (NADPH oxidase 4). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Biased expression in kidney adult (RPKM 51.3) and lung adult (RPKM 5.1) See more
    Orthologs
    NEW
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    Genomic context

    See Nox4 in Genome Data Viewer
    Location:
    7 D3; 7 48.96 cM
    Exon count:
    21
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 7 NC_000073.7 (86893638..87047918)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 7 NC_000073.6 (87244430..87398710)

    Chromosome 7 - NC_000073.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_19469 Neighboring gene vomeronasal 2, receptor 79 Neighboring gene STARR-seq mESC enhancer starr_19470 Neighboring gene STARR-seq mESC enhancer starr_19471 Neighboring gene ribosomal protein S4, X-linked pseudogene Neighboring gene STARR-seq mESC enhancer starr_19472 Neighboring gene tyrosinase Neighboring gene STARR-seq mESC enhancer starr_19473 Neighboring gene tyrosinase locus control region Neighboring gene predicted gene, 57515

    Genomic regions, transcripts, and products

    Expression

    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Variation

    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables NAD(P)H oxidase H2O2-forming activity ISO
    Inferred from Sequence Orthology
    more info
     
    enables NAD(P)H oxidase H2O2-forming activity ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    enables NADPH oxidase H202-forming activity IEA
    Inferred from Electronic Annotation
    more info
     
    enables heme binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables heme binding ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    enables modified amino acid binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables modified amino acid binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor ISO
    Inferred from Sequence Orthology
    more info
     
    enables protein tyrosine kinase binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables superoxide-generating NAD(P)H oxidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables superoxide-generating NAD(P)H oxidase activity IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    enables superoxide-generating NAD(P)H oxidase activity ISO
    Inferred from Sequence Orthology
    more info
     
    enables superoxide-generating NADPH oxidase activity IEA
    Inferred from Electronic Annotation
    more info
     
    Process Evidence Code Pubs
    acts_upstream_of_or_within bone resorption IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    acts_upstream_of_or_within cardiac muscle cell differentiation IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in cell morphogenesis IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in cellular response to glucose stimulus IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in defense response IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in gene expression IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in gene expression ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in heart process IGI
    Inferred from Genetic Interaction
    more info
    PubMed 
    involved_in heart process ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in homocysteine metabolic process IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in homocysteine metabolic process ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in negative regulation of cell population proliferation IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of DNA biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of ERK1 and ERK2 cascade IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of MAP kinase activity IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of apoptotic process ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in positive regulation of hydrogen peroxide biosynthetic process ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of protein tyrosine kinase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in positive regulation of reactive oxygen species metabolic process ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in positive regulation of smooth muscle cell migration ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in positive regulation of stress fiber assembly ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in reactive oxygen species metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in superoxide anion generation IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in superoxide anion generation IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in superoxide metabolic process ISO
    Inferred from Sequence Orthology
    more info
     
    Component Evidence Code Pubs
    part_of NADPH oxidase complex IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    part_of NADPH oxidase complex ISO
    Inferred from Sequence Orthology
    more info
     
    located_in apical plasma membrane ISO
    Inferred from Sequence Orthology
    more info
     
    located_in cell periphery IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in endoplasmic reticulum ISO
    Inferred from Sequence Orthology
    more info
     
    located_in endoplasmic reticulum ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    located_in endoplasmic reticulum membrane IEA
    Inferred from Electronic Annotation
    more info
     
    located_in endoplasmic reticulum membrane ISO
    Inferred from Sequence Orthology
    more info
     
    located_in focal adhesion IEA
    Inferred from Electronic Annotation
    more info
     
    located_in focal adhesion ISO
    Inferred from Sequence Orthology
    more info
     
    located_in mitochondrion IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in mitochondrion ISO
    Inferred from Sequence Orthology
    more info
     
    located_in nucleolus ISO
    Inferred from Sequence Orthology
    more info
     
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
     
    located_in nucleus ISO
    Inferred from Sequence Orthology
    more info
     
    located_in perinuclear endoplasmic reticulum ISO
    Inferred from Sequence Orthology
    more info
     
    located_in perinuclear region of cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in perinuclear region of cytoplasm ISO
    Inferred from Sequence Orthology
    more info
     
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in plasma membrane ISO
    Inferred from Sequence Orthology
    more info
     
    located_in plasma membrane ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    located_in sarcoplasmic reticulum ISO
    Inferred from Sequence Orthology
    more info
     
    located_in stress fiber ISO
    Inferred from Sequence Orthology
    more info
     

    General protein information

    Preferred Names
    NADPH oxidase 4
    Names
    KOX-1
    kidney oxidase-1
    kidney superoxide-producing NADPH oxidase
    renal NAD(P)H-oxidase
    superoxide-generating NADPH oxidase 4
    NP_001272762.1
    NP_001272764.1
    NP_056575.1
    XP_006508073.1
    XP_006508074.1
    XP_006508075.1
    XP_017177836.1

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001285833.1NP_001272762.1  NADPH oxidase 4 isoform 2

      See identical proteins and their annotated locations for NP_001272762.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) uses an alternate splice junction at the 5' end of an exon and contains two additional alternate coding exons compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus and an alternate internal segment compared to isoform 1.
      Source sequence(s)
      AC141329, AK149398, AK149455, BY001994, CJ227847
      Conserved Domains (2) summary
      cd06186
      Location:237542
      NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...
      pfam01794
      Location:17130
      Ferric_reduct; Ferric reductase like transmembrane component
    2. NM_001285835.1NP_001272764.1  NADPH oxidase 4 isoform 3

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) uses an alternate splice junction at the 5' end of an exon compared to variant 1. This difference causes translation initiation at a downstream AUG and results in an isoform (3) with a shorter N-terminus compared to isoform 1.
      Source sequence(s)
      AK149398, AK149455, BY001994, CJ227847
      Conserved Domains (2) summary
      cd06186
      Location:237503
      NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...
      pfam01794
      Location:17130
      Ferric_reduct; Ferric reductase like transmembrane component
    3. NM_015760.5NP_056575.1  NADPH oxidase 4 isoform 1

      See identical proteins and their annotated locations for NP_056575.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes the longest isoform (1).
      Source sequence(s)
      AK050371, AK149398, AK149455, BY001994, CJ227847
      Consensus CDS
      CCDS21437.1
      UniProtKB/Swiss-Prot
      Q3TF39, Q8C3M1, Q8VCA3, Q9JHI8
      UniProtKB/TrEMBL
      B2RSM1
      Related
      ENSMUSP00000032781.8, ENSMUST00000032781.14
      Conserved Domains (2) summary
      cd06186
      Location:311577
      NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...
      pfam01794
      Location:69204
      Ferric_reduct; Ferric reductase like transmembrane component

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000073.7 Reference GRCm39 C57BL/6J

      Range
      86893638..87047918
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006508012.2XP_006508075.1  NADPH oxidase 4 isoform X4

      UniProtKB/Swiss-Prot
      Q9JHI8
      Conserved Domains (2) summary
      cd06186
      Location:311616
      NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...
      pfam01794
      Location:69204
      Ferric_reduct; Ferric reductase like transmembrane component
    2. XM_017322347.2XP_017177836.1  NADPH oxidase 4 isoform X1

      Conserved Domains (2) summary
      cd06186
      Location:311576
      NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...
      pfam01794
      Location:69204
      Ferric_reduct; Ferric reductase like transmembrane component
    3. XM_006508011.4XP_006508074.1  NADPH oxidase 4 isoform X3

      Conserved Domains (2) summary
      pfam01794
      Location:69204
      Ferric_reduct; Ferric reductase like transmembrane component
      cl06868
      Location:307357
      FNR_like; Ferredoxin reductase (FNR), an FAD and NAD(P) binding protein, was intially identified as a chloroplast reductase activity, catalyzing the electron transfer from reduced iron-sulfur protein ferredoxin to NADP+ as the final step in the electron transport ...
    4. XM_006508010.4XP_006508073.1  NADPH oxidase 4 isoform X2

      Conserved Domains (2) summary
      cd06186
      Location:294560
      NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...
      pfam01794
      Location:52187
      Ferric_reduct; Ferric reductase like transmembrane component

    RNA

    1. XR_004934139.1 RNA Sequence