SSK1 mitogen-activated protein kinase kinase kinase SSK1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SSK1
Official Full Name: mitogen-activated protein kinase kinase kinase SSK1
Primary source: SGD:S000003996
Locus tag: YLR006C
See related: AllianceGenome:SGD:S000003996; FungiDB:YLR006C; VEuPathDB:YLR006C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables mitogen-activated protein kinase kinase kinase binding activity; phosphorelay response regulator activity; and protein kinase activator activity. Involved in several processes, including cellular response to acidic pH; cellular response to osmotic stress; and regulation of p38MAPK cascade. Located in cytoplasm. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See SSK1 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (161755..163893, complement)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

In silico free energy estimates, and sequence comparisons reveal distinctive electrostatic properties of the Ypd1/Ssk1-R2-W638A complex that may provide insight into the regulation of the Sln1 pathway as a function of dynamic osmolyte concentration.
Title: Insights revealed by the co-crystal structure of the Saccharomyces cerevisiae histidine phosphotransfer protein Ypd1 and the receiver domain of its downstream response regulator Ssk1.
Delayed turnover of unphosphorylated Ssk1 during carbon stress activates the yeast Hog1 map kinase pathway.
Title: Delayed Turnover of Unphosphorylated Ssk1 during Carbon Stress Activates the Yeast Hog1 Map Kinase Pathway.
Our data suggest that Snf1 downregulates ER stress response signal mediated by Hog1 through negatively regulating expression of its specific activator Ssk1 at the transcriptional level
Title: The Saccharomyces cerevisiae AMPK, Snf1, Negatively Regulates the Hog1 MAPK Pathway in ER Stress Response.
network structure of the Sln1-Ypd1-Ssk1 three-component phospho-relay prevents unintended activation of the HOG MAPK pathway in Saccharomyces cerevisiae
Title: Robust network structure of the Sln1-Ypd1-Ssk1 three-component phospho-relay prevents unintended activation of the HOG MAPK pathway in Saccharomyces cerevisiae.
The osmotic stress response through the Ssk1p-independent Ssk2p activation is strong, although its duration is short compared with the Ssk1p-dependent activation.
Title: Ssk1p-independent activation of Ssk2p plays an important role in the osmotic stress response in Saccharomyces cerevisiae: alternative activation of Ssk2p in osmotic stress.
Ssk1 is required for Hog1 metabolic activation.
Title: Metabolic activation of the HOG MAP kinase pathway by Snf1/AMPK regulates lipid signaling at the Golgi.
Osmolytes negatively affect the stability of the SSK1-3YPD1 complex in a concentration-dependent manner and, as a consequence, the rate of phosphate hydrolysis increases.
Title: Effects of osmolytes on the SLN1-YPD1-SSK1 phosphorelay system from Saccharomyces cerevisiae.
Constitutive activation of Ssk2 occurs when Ssk1 phosphorylation is blocked by either an Ssk1 mutation at the phosphorylation site or an Ssk1 mutation that inhibits its interaction with Ypd1, the donor of phosphate to Ssk1.
Title: Phosphorylated Ssk1 prevents unphosphorylated Ssk1 from activating the Ssk2 mitogen-activated protein kinase kinase kinase in the yeast high-osmolarity glycerol osmoregulatory pathway.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables mitogen-activated protein kinase kinase kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables phosphorelay response regulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphorelay response regulator activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein kinase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activator activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular hyperosmotic response	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular hyperosmotic response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to acidic pH	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in osmosensory signaling via phosphorelay pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in osmosensory signaling via phosphorelay pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphorelay signal transduction system	IEA Inferred from Electronic Annotation more info
involved_in regulation of actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of p38MAPK cascade	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of p38MAPK cascade	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of p38MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: mitogen-activated protein kinase kinase kinase SSK1

NP_013106.1

Cytoplasmic phosphorelay intermediate osmosensor and regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

161755..163893 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181893.1 → NP_013106.1 TPA: mitogen-activated protein kinase kinase kinase SSK1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013106.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VY08, Q07084, Q07909

UniProtKB/TrEMBL

A0A142F3P3, A7A0R1, B3LSX9, N1P0D0

Conserved Domains (1) summary

cd17546
Location:506 → 643

REC_hyHK_CKI1_RcsC-like; phosphoacceptor receiver (REC) domain of hybrid sensor histidine kinases/response regulators similar to Arabidopsis thaliana CKI1 and Escherichia coli RcsC