DSN1 MIND complex subunit DSN1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DSN1
Official Full Name: MIND complex subunit DSN1
Primary source: SGD:S000001449
Locus tag: YIR010W
See related: AllianceGenome:SGD:S000001449; FungiDB:YIR010W; VEuPathDB:YIR010W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Involved in spindle attachment to meiosis I kinetochore. Located in kinetochore and spindle pole. Part of MIS12/MIND type complex. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See DSN1 in Genome Data Viewer

Location:: chromosome: IX

Exon count:: 1

Sequence:: Chromosome: IX; NC_001141.2 (375431..377161)

Chromosome IX - NC_001141.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001141.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Csm1 binds two conserved motifs in Dsn1, one (termed Box 1) representing the ancestral, widely conserved monopolin binding motif and a second (termed Box 2-3) with a likely role in enforcing specificity of sister kinetochore crosslinking. We find that Box 1 and Box 2-3 bind the same conserved hydrophobic cavity on Csm1, suggesting competition or handoff between these motifs. More information in the paper.
Title: The molecular basis of monopolin recruitment to the kinetochore.
We used this assay to investigate why kinetochores recruit the microtubule-binding Ndc80 complex via two receptors: the Mis12 complex and CENP-T. Although the CENP-T pathway is non-essential in yeast, we demonstrate that it becomes essential for viability and Ndc80c recruitment when the Mis12 pathway is crippled by defects in Dsn1 phosphorylation
Title: An assay for de novo kinetochore assembly reveals a key role for the CENP-T pathway in budding yeast.
Altogether, these data indicate that the absence of R37 methylation on Cse4 reduces the recruitment of kinetochore proteins to centromeric chromatin, and that this can be compensated for by stabilizing the outer kinetochore protein Dsn1 via the ubiquitin ligase complex Ubr2/Mub1.
Title: Defects in methylation of arginine 37 on CENP-A/Cse4 are compensated by the ubiquitin ligase complex Ubr2/Mub1.
We found that Aurora B phosphorylation of Dsn1 promotes the interaction between outer and inner kinetochore proteins in budding yeast.
Title: The aurora B kinase promotes inner and outer kinetochore interactions in budding yeast.
Consistent with this, Mub1/Ubr2 help to maintain viability when kinetochores are defective. Together, our data identify a previously unknown regulatory mechanism for the conserved Dsn1 kinetochore protein
Title: The Mub1/Ubr2 ubiquitin ligase complex regulates the conserved Dsn1 kinetochore protein.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables molecular_function	ND No biological Data available more info

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	IDA Inferred from Direct Assay more info	PubMed
involved_in chromosome segregation	IEA Inferred from Electronic Annotation more info
involved_in spindle attachment to meiosis I kinetochore	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of MIS12/MIND type complex	IBA Inferred from Biological aspect of Ancestor more info
part_of MIS12/MIND type complex	IDA Inferred from Direct Assay more info	PubMed
part_of MIS12/MIND type complex	IEA Inferred from Electronic Annotation more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in kinetochore	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in spindle pole	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: MIND complex subunit DSN1

NP_012275.3

Essential component of the outer kinetochore MIND complex; joins kinetochore subunits contacting DNA to those contacting microtubules; phosphorylation promotes interaction between outer and inner kinetochore proteins; kinetochore receptor for monopolin, via interaction with Csm1p; essential for both meiotic and mitotic chromosome segregation; MIND complex consists of Mtw1p, Nnf1p, Nsl1p and Dsn1p; phosphorylated by monopolin subunit, Hrr25p and Aurora kinase, Ipl1p; modified by sumoylation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001141.2 Reference assembly

Range

375431..377161

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179532.3 → NP_012275.3 TPA: MIND complex subunit DSN1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012275.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VVU0, P40568

UniProtKB/TrEMBL

B3LTJ9, N1P0Q8

Conserved Domains (1) summary

pfam08202
Location:221 → 569

MIS13; Mis12-Mtw1 protein family