MSN4 stress-responsive transcriptional activator MSN4 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MSN4
Official Full Name: stress-responsive transcriptional activator MSN4
Primary source: SGD:S000001545
Locus tag: YKL062W
See related: AllianceGenome:SGD:S000001545; FungiDB:YKL062W; VEuPathDB:YKL062W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA-binding transcription factor activity, RNA polymerase II-specific and cis-regulatory region sequence-specific DNA binding activity. Involved in several processes, including cellular response to ethanol; cellular response to freezing; and cellular response to pH. Located in cytosol and nucleus. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See MSN4 in Genome Data Viewer

Location:: chromosome: XI

Exon count:: 1

Sequence:: Chromosome: XI; NC_001143.9 (323228..325120)

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Deletion of the transcription factors Hsf1, Msn2 and Msn4 in yeast uncovers transcriptional reprogramming in response to proteotoxic stress.
Title: Deletion of the transcription factors Hsf1, Msn2 and Msn4 in yeast uncovers transcriptional reprogramming in response to proteotoxic stress.
Msn2/4 transcription factors positively regulate expression of Atg39 ER-phagy receptor.
Title: Msn2/4 transcription factors positively regulate expression of Atg39 ER-phagy receptor.
This work attempts to reveal the significance of specific serine residues of Msn2/4p, as possible targets of PKA, through substitution of these serine residues with alanine.
Title: Specific serine residues of Msn2/4 are responsible for regulation of alcohol fermentation rates and ethanol resistance.
Msn2 and Msn4 promote ATG8 expression downstream of the histone deacetylase complex (HDAC) subunit Ume6, a previously identified negative regulator of ATG8 expression.
Title: Stress-response transcription factors Msn2 and Msn4 couple TORC2-Ypk1 signaling and mitochondrial respiration to ATG8 gene expression and autophagy.
e transcription factors also play an important role in the exit of quiescence and regrowth. By studying Saccharomyces cerevisiae under a continuous, nutrient-limited condition, we found that Msn2 and Msn4 function as master regulators of glycolytic genes in the quiescent-like phase.
Title: Stress response factors drive regrowth of quiescent cells.
Thus Msn2/4 exhibit a dual role in activating carbohydrate metabolism genes and stress response genes.
Title: Msn2/4 regulate expression of glycolytic enzymes and control transition from quiescence to growth.
Data suggest that promoters of most genes involved in biogenesis, function, and regulation of peroxisomes contain msn2- and msn4-binding sites; msn2 exhibits increased occupancy on promoters of beta-oxidation genes in glucose-depleted conditions; thus, msn2 and msn4 transcription factors activate expression of genes involved in fatty acid oxidation.
Title: The stress-regulatory transcription factors Msn2 and Msn4 regulate fatty acid oxidation in budding yeast.
Target genes of Msn2 and Msn4 with fast activation kinetics can be fully induced by either factor, behaving as an 'OR' gate. In contrast, target genes with slow activation kinetics behave as an 'AND' gate, requiring distinct contributions from both factors, upon transient stimulation.
Title: Dynamic control of gene regulatory logic by seemingly redundant transcription factors.
Study demonstrate that Rpa12p interacts with the transcription factor Msn4p and prevents its binding to the promoter of AYR1, a key enzyme involved in triacylglycerol biosynthesis and mobilization of nonpolar lipids. These results suggest that the deletion of RPA12 derepresses Msn4p, thus activating the transcription of AYR1, which leads to the accumulation of triacylglycerol in the rpa12D mutant.
Title: The RNA polymerase I subunit Rpa12p interacts with the stress-responsive transcription factor Msn4p to regulate lipid metabolism in budding yeast.
Cdc55 phosphatase is not involved in cytosolic stress signal perception but is involved in a specific intranuclear mechanism to regulate Msn2 and Msn4 nuclear accumulation and chromatin association under stress conditions.
Title: Yeast protein phosphatase 2A-Cdc55 regulates the transcriptional response to hyperosmolarity stress by regulating Msn2 and Msn4 chromatin recruitment.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IMP Inferred from Mutant Phenotype more info	PubMed
enables cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular hypotonic response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to alkaline pH	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to amino acid starvation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to arsenic-containing substance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to cold	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to ethanol	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to freezing	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to glucose starvation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to heat	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to heat	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to hydrogen peroxide	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to hydrogen peroxide	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to hydrostatic pressure	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to nitrogen starvation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to nitrosative stress	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to salt	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to zinc ion starvation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular stress response to acidic pH	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular stress response to acidic pH	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin remodeling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to starvation	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: stress-responsive transcriptional activator MSN4

NP_012861.1

Stress-responsive transcriptional activator; activated in stochastic pulses of nuclear localization in response to various stress conditions; binds DNA at stress response elements of responsive genes, inducing gene expression; involved in diauxic shift

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9 Reference assembly

Range

323228..325120

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179628.1 → NP_012861.1 TPA: stress-responsive transcriptional activator MSN4 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012861.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VXM5, P33749, P35726

UniProtKB/TrEMBL

A0A3G9D666, B3LR29, B5VM94, C7GP12, N1P090

Conserved Domains (2) summary

COG5048
Location:57 → 622

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:575 → 596

ZF_C2H2; C2H2 Zn finger [structural motif]