DHX33 DEAH-box helicase 33 [Homo sapiens (human)] - Gene

Summary

Official Symbol: DHX33provided by HGNC
Official Full Name: DEAH-box helicase 33provided by HGNC
Primary source: HGNC:HGNC:16718
See related: Ensembl:ENSG00000005100 MIM:614405; AllianceGenome:HGNC:16718
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: DDX33
Summary: This gene encodes a member of the DEAD box protein family. The DEAD box proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
Expression: Ubiquitous expression in brain (RPKM 5.2), appendix (RPKM 3.8) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 17p13.2

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	17	NC_000017.11 (5440917..5468982, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	17	NC_060941.1 (5334655..5362725, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	17	NC_000017.10 (5344237..5372302, complement)

Chromosome 17 - NC_000017.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

RNA helicase DHX33 regulates HMGB family genes in human cancer cells.
Title: RNA helicase DHX33 regulates HMGB family genes in human cancer cells.
Systematic analysis of the prognostic and immunological role of DHX33 in pan-cancer.
Title: Systematic analysis of the prognostic and immunological role of DHX33 in pan-cancer.
Comprehensive analysis of immune implication and prognostic value of DHX33 in sarcoma.
Title: Comprehensive analysis of immune implication and prognostic value of DHX33 in sarcoma.
GSK-3beta phosphorylation of DHX33 leads to its ubiquitination mediated protein degradation.
Title: GSK-3β phosphorylation of DHX33 leads to its ubiquitination mediated protein degradation.
Long non-coding RNA HOTAIR promotes hepatocellular carcinoma progression by regulating miR-526b-3p/DHX33 axis.
Title: Long non-coding RNA HOTAIR promotes hepatocellular carcinoma progression by regulating miR-526b-3p/DHX33 axis.
Function of DHX33 in promoting Warburg effect via regulation of glycolytic genes.
Title: Function of DHX33 in promoting Warburg effect via regulation of glycolytic genes.
DHX33 Recruits Gadd45a To Cause DNA Demethylation and Regulates a Subset of Gene Transcription.
Title: DHX33 Recruits Gadd45a To Cause DNA Demethylation and Regulates a Subset of Gene Transcription.
This study highlights that DHX33 protein is critical in glioblastoma cell proliferation and its potential as a novel therapeutic drug target.
Title: The RNA helicase DHX33 is required for cancer cell proliferation in human glioblastoma and confers resistance to PI3K/mTOR inhibition.
DHX33 is highly expressed in human colon cancers. DHX33 and Wnt/beta-catenin reciprocally regulate each other.
Title: DHX33 promotes colon cancer development downstream of Wnt signaling.
Results reveal that DHX33 represses apoptosis through the direct upregulation of Bcl-2 gene transcription. AP-2beta is a binding partner for DHX33 and that DHX33 acts as a coactivator for AP-2beta to promote the transcription of antiapoptotic Bcl-2 gene.
Title: DHX33 Interacts with AP-2β To Regulate Bcl-2 Gene Expression and Promote Cancer Cell Survival.

Submit: New GeneRIF Correction See all GeneRIFs (21)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Replication interactions

Interaction	Pubs
Knockdown of DEAH (Asp-Glu-Ala-His) box polypeptide 33 (DHX33) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D10S275 (e-PCR)
- UniSTS:147992

STS-R63801 (e-PCR)
- UniSTS:45701

D11S3114 (e-PCR)
- UniSTS:152207

RH36080 (e-PCR)
- UniSTS:62396

RH80463 (e-PCR)
- UniSTS:83679

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor binding	IEA Inferred from Electronic Annotation more info
enables RNA binding	HDA more info	PubMed
enables RNA helicase activity	IEA Inferred from Electronic Annotation more info
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables double-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables double-stranded RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables rDNA binding	IDA Inferred from Direct Assay more info	PubMed
enables ribosomal large subunit binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of MAPK cascade	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of NF-kappaB transcription factor activity	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of NLRP3 inflammasome complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase I	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription by RNA polymerase I	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of type I interferon production	ISS Inferred from Sequence or Structural Similarity more info
involved_in translational initiation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of NLRP3 inflammasome complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleolus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ATP-dependent RNA helicase DHX33

Names: DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 33; DEAH (Asp-Glu-Ala-His) box polypeptide 33; DEAH box protein 33; putative ATP-dependent RNA helicase DHX33

NP_001186628.1

EC 3.6.4.13

NP_064547.2

EC 3.6.4.13

XP_016880366.1

EC 3.6.4.13

XP_047292374.1

EC 3.6.4.13

XP_054172671.1

EC 3.6.4.13

XP_054172672.1

EC 3.6.4.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001199699.2 → NP_001186628.1 ATP-dependent RNA helicase DHX33 isoform 2

See identical proteins and their annotated locations for NP_001186628.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an exon in the 5' coding region and uses a downstream start codon, compared to variant 1. The resulting protein (isoform 2) has a shorter N-terminus, compared to isoform 1.

Source sequence(s)

AC004148, AK295760, BC030017, BQ775963

UniProtKB/TrEMBL

I3L1L6

Related

ENSP00000458925.1, ENST00000572490.1

Conserved Domains (5) summary

smart00847
Location:305 → 390

HA2; Helicase associated domain (HA2) Add an annotation

COG1643
Location:1 → 522

HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]

cd00046
Location:1 → 61

DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.

pfam00271
Location:118 → 235

Helicase_C; Helicase conserved C-terminal domain

pfam07717
Location:424 → 524

OB_NTP_bind; Oligonucleotide/oligosaccharide-binding (OB)-fold
NM_020162.4 → NP_064547.2 ATP-dependent RNA helicase DHX33 isoform 1

See identical proteins and their annotated locations for NP_064547.2

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC004148, AK295760, BQ775963

Consensus CDS

CCDS11072.1

UniProtKB/Swiss-Prot

B4DHF9, Q4G149, Q5CZ73, Q9H5M9, Q9H6R0

UniProtKB/TrEMBL

B4DIS6

Related

ENSP00000225296.3, ENST00000225296.8

Conserved Domains (5) summary

smart00847
Location:478 → 563

HA2; Helicase associated domain (HA2) Add an annotation

COG1643
Location:67 → 695

HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]

cd00046
Location:92 → 234

DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.

pfam00271
Location:291 → 408

Helicase_C; Helicase conserved C-terminal domain

pfam07717
Location:597 → 697

OB_NTP_bind; Oligonucleotide/oligosaccharide-binding (OB)-fold

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000017.11 Reference GRCh38.p14 Primary Assembly

Range

5440917..5468982 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017024877.2 → XP_016880366.1 ATP-dependent RNA helicase DHX33 isoform X2

UniProtKB/Swiss-Prot

Q9H6R0

Conserved Domains (1) summary

COG1643
Location:1 → 267

HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]
XM_047436418.1 → XP_047292374.1 ATP-dependent RNA helicase DHX33 isoform X1