SAE2 ssDNA endodeoxyribonuclease SAE2 [Saccharomyces cerevisiae S288C] - Gene

Summary

Go to the top of the page Help

Official Symbol: SAE2
Official Full Name: ssDNA endodeoxyribonuclease SAE2
Primary source: SGD:S000003143
Locus tag: YGL175C
See related: AllianceGenome:SGD:S000003143; FungiDB:YGL175C; VEuPathDB:YGL175C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: COM1
Summary: Enables double-stranded DNA binding activity and single-stranded DNA endodeoxyribonuclease activity. Involved in DNA metabolic process. Acts upstream of or within DNA catabolic process. Located in cytoplasm and nucleus. Part of Mre11 complex. [provided by Alliance of Genome Resources, Dec 2024]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See SAE2 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (173285..174322, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nej1 interacts with Sae2 at DNA double-stranded breaks to inhibit DNA resection.
Title: Nej1 interacts with Sae2 at DNA double-stranded breaks to inhibit DNA resection.
sae2-ms mutant upregulates Mre11-Rad50-Xrs2 complex and Tel1 signaling activities at DSBs by reducing Mre11 endonuclease activity.
Title: Uncoupling Sae2 Functions in Downregulation of Tel1 and Rad53 Signaling Activities.
Overexpression of the RNA-DNA helicase Senataxin suppresses DNA damage sensitivity and R-loop accumulation in Sae2/CtIP-deficient cells, and a catalytic mutant of CtIP fails to complement this sensitivity, indicating a role for CtIP nuclease activity in the repair process.
Title: Sae2/CtIP prevents R-loop accumulation in eukaryotic cells.
Phosphorylation of S. cerevisiae ssDNA endodeoxyribonuclease SAE2 controls its capacity to promote the Mre11-Rad50-Xrs2 (MRX) nuclease to initiate resection of blocked DNA ends. DNA damage and cell cycle-dependent phosphorylation leads to Sae2 tetramerization. Phosphorylation of the conserved C-terminal domain of Sae2 is a prerequisite for its interaction with the MRX subunit Rad50.
Title: Regulatory control of DNA end resection by Sae2 phosphorylation.
MRX-Sae2 efficiently cleaves near Ku-blocked DNA ends, which shows how recombination competes with NHEJ in the S/G2 phase when Sae2 is phosphorylated and thus capable of promoting endonucleolytic DNA cleavage by MRX
Title: Physiological protein blocks direct the Mre11-Rad50-Xrs2 and Sae2 nuclease complex to initiate DNA end resection.
Authors find that the ability of Sae2 to promote MRX nuclease functions is important for DNA damage survival, particularly in the absence of Dna2 nuclease activity.
Title: Genetic Separation of Sae2 Nuclease Activity from Mre11 Nuclease Functions in Budding Yeast.
CtIP/Ctp1/Sae2/Com1 role in removal of DNA double strand breaks through DSB repair by homologous recombination is reviewed.
Title: CtIP/Ctp1/Sae2, molecular form fit for function.
our data suggest a direct role for Sae2 nuclease activity in processing of the DNA structures that arise during replication and DNA damage and provide insights into the mechanism underlying Mre11-Sae2-mediated abrogation of replication stressrelated defects in S. cerevisiae.
Title: Genetic and biochemical evidences reveal novel insights into the mechanism underlying Saccharomyces cerevisiae Sae2-mediated abrogation of DNA replication stress.
By performing a genetic screen, we identify rad53 and tel1 mutant alleles that suppress both the DNA damage hypersensitivity and the resection defect of sae2Delta cells through an Sgs1-Dna2-dependent mechanism
Title: Sae2 Function at DNA Double-Strand Breaks Is Bypassed by Dampening Tel1 or Rad53 Activity.
secondary structures within single-stranded DNA are potent instigators of genome instability, and RPA and Mre11-Sae2 play important roles in preventing their formation and propagation, respectively.
Title: Mre11-Sae2 and RPA Collaborate to Prevent Palindromic Gene Amplification.

Submit: New GeneRIF Correction See all GeneRIFs (34)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables endonuclease activity	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA endodeoxyribonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA endodeoxyribonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA catabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within DNA catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA double-strand break processing	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA double-strand break processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA double-strand break processing involved in repair via synthesis-dependent strand annealing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in gene conversion at mating-type locus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic DNA double-strand break formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in meiotic DNA double-strand break processing	IGI Inferred from Genetic Interaction more info	PubMed
involved_in meiotic DNA double-strand break processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in telomere maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomeric 3' overhang formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Mre11 complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: ssDNA endodeoxyribonuclease SAE2

NP_011340.1

Endonuclease required for telomere elongation; required for telomeric 5' C-rich strand resection; involved in ds-break repair and processing hairpin DNA structures with the MRX complex; function requires sumoylation and phosphorylation; exists as inactive oligomers that are transiently released into smaller active units by phosphorylation; DNA damage triggers Sae2p removal, so active Sae2p is present only transiently; sequence and functional similarity with human CtIP/RBBP8