TPO1 polyamine transporter TPO1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: TPO1
Official Full Name: polyamine transporter TPO1
Primary source: SGD:S000003951
Locus tag: YLL028W
See related: AllianceGenome:SGD:S000003951; FungiDB:YLL028W; VEuPathDB:YLL028W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables spermidine transmembrane transporter activity and spermine transmembrane transporter activity. Involved in cellular response to oxidative stress and polyamine transport. Located in cellular bud membrane and fungal-type vacuole membrane. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See TPO1 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (84804..86564)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

results described suggest that Tpo1 is one of the key players standing in the crossroad between benzoic acid stress response and tolerance and the control of the intracellular concentration of nitrogenous compounds
Title: Yeast response and tolerance to benzoic acid involves the Gcn4- and Stp1-regulated multidrug/multixenobiotic resistance transporter Tpo1.
The present study provides evidence that alterations in the sphingolipid level/structure result in loss of Tpo1 protein from the mother at each cell division leading to a progressive decline in the total protein level in mother.
Title: Sphingolipids facilitate age asymmetry of membrane proteins in dividing yeast cells.
TPO1 times induction of oxidation stress response proteins and cell cycle arrest.
Title: Tpo1-mediated spermine and spermidine export controls cell cycle delay and times antioxidant protein expression during the oxidative stress response.
YAP1 overexpression rescues the imp2Delta mutant from bleomycin toxicity by triggering Tpo1 expression to expel the drug.
Title: Upregulation of the Saccharomyces cerevisiae efflux pump Tpo1 rescues an Imp2 transcription factor-deficient mutant from bleomycin toxicity.
The timing of TPO1 expression during the cell cycle is critical to the observed localization patterns, asymmetric localization of Tpo1 to the mother cortex.
Title: Asymmetrically inherited multidrug resistance transporters are recessive determinants in cellular replicative ageing.
The transport activity of TPO1 was enhanced through phosphorylation at Ser19 by protein kinase C and at Thr52 by casein kinase 1
Title: Characteristics of the polyamine transporter TPO1 and regulation of its activity and cellular localization by phosphorylation.
The transcriptional activation of TPO1 and PDR5 was registered after exposure of the unadapted yeast population to acute artesunate-induced stress, being significantly reduced in the absence of Pdr1p and abolished in the absence of Pdr1p and Pdr3p.
Title: Adaptive response to the antimalarial drug artesunate in yeast involves Pdr1p/Pdr3p-mediated transcriptional activation of the resistance determinants TPO1 and PDR5.
We screened for Saccharomyces cerevisiae genes whose overexpression causes indomethacin resistance and identified the TPO1 gene, which encodes a major facilitator superfamily transporter.
Title: Identification of the TPO1 gene in yeast, and its human orthologue TETRAN, which cause resistance to NSAIDs.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables antiporter activity	IEA Inferred from Electronic Annotation more info
enables spermidine transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables spermidine transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables spermine transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables spermine transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables transmembrane transporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cellular response to oxidative stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in polyamine transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in putrescine transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermidine transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in spermidine transport	IDA Inferred from Direct Assay more info	PubMed
involved_in spermidine transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermine transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in spermine transport	IDA Inferred from Direct Assay more info	PubMed
involved_in spermine transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cell periphery	HDA more info	PubMed
located_in cellular bud membrane	IDA Inferred from Direct Assay more info	PubMed
located_in fungal-type vacuole membrane	IMP Inferred from Mutant Phenotype more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: polyamine transporter TPO1

NP_013072.1

Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; during oxidative stress exports spermine, spermidine from the cell, which controls timing of expression of stress-responsive genes; phosphorylation enhances activity and sorting to the plasma membrane

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

84804..86564

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181848.1 → NP_013072.1 TPA: polyamine transporter TPO1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013072.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VXX6, Q07824

UniProtKB/TrEMBL

B3LTE2, N1NZX9

Conserved Domains (1) summary

cd17323
Location:156 → 577

MFS_Tpo1_MDR_like; Yeast Polyamine transporter 1 (Tpo1) and similar multidrug resistance (MDR) transporters of the Major Facilitator Superfamily