MSK1 lysine--tRNA ligase MSK1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MSK1
Official Full Name: lysine--tRNA ligase MSK1
Primary source: SGD:S000005017
Locus tag: YNL073W
See related: AllianceGenome:SGD:S000005017; FungiDB:YNL073W; VEuPathDB:YNL073W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables lysine-tRNA ligase activity. Involved in mitochondrial lysyl-tRNA aminoacylation and tRNA processing. Located in mitochondrion. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See MSK1 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (488386..490116)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

one out of two cytosolic lysine tRNAs of the yeast Saccharomyces cerevisiae is partially internalized by mitochondria. The key protein factor of this process is the precursor of mitochondrial lysyl-tRNA synthetase, preMsk1p.
Title: Procedure for Purification of Recombinant preMsk1p from E. coli Determines Its Properties as a Factor of tRNA Import into Yeast Mitochondria.
In this work, the authors identify the hinge region with two adjacent helices H5 and H7 of preMSK1 to be responsible for mitochondrial targeting of the tRNA(Lys) and characterize preMsk1p versions with altered tRK1 import capacities.
Title: tRNA mitochondrial import in yeast: Mapping of the import determinants in the carrier protein, the precursor of mitochondrial lysyl-tRNA synthetase.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables aminoacyl-tRNA ligase activity	IEA Inferred from Electronic Annotation more info
enables lysine-tRNA ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables lysine-tRNA ligase activity	IEA Inferred from Electronic Annotation more info
enables lysine-tRNA ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables lysine-tRNA ligase activity	ISA Inferred from Sequence Alignment more info	PubMed
enables tRNA binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in lysyl-tRNA aminoacylation	IEA Inferred from Electronic Annotation more info
involved_in lysyl-tRNA aminoacylation	ISA Inferred from Sequence Alignment more info	PubMed
involved_in mitochondrial lysyl-tRNA aminoacylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrial lysyl-tRNA aminoacylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tRNA aminoacylation for protein translation	IEA Inferred from Electronic Annotation more info
involved_in tRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in translation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in mitochondrial matrix	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: lysine--tRNA ligase MSK1

NP_014326.1

EC 6.1.1.6

Mitochondrial lysine-tRNA synthetase; required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys)

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

488386..490116

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182911.1 → NP_014326.1 TPA: lysine--tRNA ligase MSK1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014326.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W1A6, P32048

UniProtKB/TrEMBL

B3LNT2, B5VQX2, N1NWM2

Conserved Domains (1) summary

TIGR00499
Location:41 → 573

lysS_bact; lysyl-tRNA synthetase, eukaryotic and non-spirochete bacterial