Myf5 myogenic factor 5 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Myf5provided by MGI
Official Full Name: myogenic factor 5provided by MGI
Primary source: MGI:MGI:97252
See related: Ensembl:ENSMUSG00000000435 AllianceGenome:MGI:97252
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Myf-5; bHLHc2; B130010J22Rik
Summary: Enables DNA-binding transcription activator activity, RNA polymerase II-specific and sequence-specific DNA binding activity. Acts upstream of or within several processes, including positive regulation of transcription by RNA polymerase II; skeletal muscle cell differentiation; and skeletal system morphogenesis. Predicted to be located in nucleoplasm. Is expressed in several structures, including alimentary system; brain; embryo mesenchyme; genitourinary system; and musculature. Orthologous to human MYF5 (myogenic factor 5). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in limb E14.5 (RPKM 2.7), ovary adult (RPKM 0.6) and 1 other tissue See more
Orthologs: human all
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Genomic context

Location:: 10 D1; 10 55.95 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (107318769..107322787, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (107482908..107486927, complement)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A dual-color PAX7 and MYF5 in vivo reporter to investigate muscle stem cell heterogeneity in regeneration and aging.
Title: A dual-color PAX7 and MYF5 in vivo reporter to investigate muscle stem cell heterogeneity in regeneration and aging.
Dnmt3b Deficiency in Myf5(+)-Brown Fat Precursor Cells Promotes Obesity in Female Mice.
Title: Dnmt3b Deficiency in Myf5(+)-Brown Fat Precursor Cells Promotes Obesity in Female Mice.
PUFA Treatment Affects C2C12 Myocyte Differentiation, Myogenesis Related Genes and Energy Metabolism.
Title: PUFA Treatment Affects C2C12 Myocyte Differentiation, Myogenesis Related Genes and Energy Metabolism.
MLL1 facilitates proliferation of myoblasts and Pax7-positive satellite cells by epigenetically regulating Myf5.
Title: MLL1 promotes myogenesis by epigenetically regulating Myf5.
The induction phase of primary Myf5+ brown adipogenesis.
Title: STAT3 suppresses Wnt/β-catenin signaling during the induction phase of primary Myf5+ brown adipogenesis.
Asymmetrically dividing muscle stem cells in skeletal muscle give rise to committed cells, where the myogenic determination factor Myf5 is transcriptionally activated by Pax7.
Title: The Dystrophin Glycoprotein Complex Regulates the Epigenetic Activation of Muscle Stem Cell Commitment.
An absolute requirement for either MyoD or Myf5 in muscle regeneration.
Title: Loss of MyoD and Myf5 in Skeletal Muscle Stem Cells Results in Altered Myogenic Programming and Failed Regeneration.
Novel RNA-binding activity of MYF5 enhances Ccnd1 mRNA translation during myogenesis.
Title: Novel RNA-binding activity of MYF5 enhances Ccnd1/Cyclin D1 mRNA translation during myogenesis.
Transcription of the skeletal muscle program is achieved by the expression of MyoD, which binds to the same sites as Myf5, indicating that each factor regulates distinct steps in gene initiation and transcription at a shared set of binding sites
Title: Distinct Activities of Myf5 and MyoD Indicate Separate Roles in Skeletal Muscle Lineage Specification and Differentiation.
The Myf5- and Myogenin-deficient mice showed a partial or complete, respectively, loss of TMC in an otherwise regularly structured thymus.
Title: Myf5 and Myogenin in the development of thymic myoid cells - Implications for a murine in vivo model of myasthenia gravis.

Submit: New GeneRIF Correction See all GeneRIFs (51)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (7) 1 citation
Targeted (21) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PMC15073P2 (e-PCR)
- UniSTS:158744

Myf5 (e-PCR), detects polymorphism
- UniSTS:158745

NoName (e-PCR)
- UniSTS:548382

Myf5 (e-PCR), detects polymorphism
- UniSTS:238139

Myf5 (e-PCR), detects polymorphism
- UniSTS:143943

Myf5 (e-PCR), detects polymorphism
- UniSTS:143944

Myf5 (e-PCR), detects polymorphism
- UniSTS:143945

Myf5 (e-PCR), detects polymorphism
- UniSTS:238164

Myf5 (e-PCR), detects polymorphism
- UniSTS:226142

Myf5 (e-PCR), detects polymorphism
- UniSTS:525925

NoName (e-PCR)
- UniSTS:548218

Myf5 (e-PCR), detects polymorphism
- UniSTS:545961

Myf5 (e-PCR), detects polymorphism
- UniSTS:507078

Myf5 (e-PCR), detects polymorphism
- UniSTS:233980

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	TAS Traceable Author Statement more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within camera-type eye development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cartilage condensation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within embryonic skeletal system morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within extracellular matrix organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within muscle organ development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within muscle organ development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within muscle organ morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within muscle tissue morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within muscle tissue morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within ossification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of myoblast differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of skeletal muscle fiber development	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of cell-matrix adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in skeletal muscle cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within skeletal muscle cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within skeletal muscle tissue development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within skeletal muscle tissue development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within somitogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	TAS Traceable Author Statement more info	PubMed

General protein information

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Preferred Names: myogenic factor 5

Names: myogenic regulatory factor MYF-5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_008656.5 → NP_032682.1 myogenic factor 5

See identical proteins and their annotated locations for NP_032682.1

Status: VALIDATED

Source sequence(s)

AK044894, AK083402

Consensus CDS

CCDS24161.1

UniProtKB/Swiss-Prot

P24699, Q543W7

UniProtKB/TrEMBL

A2RSK4

Related

ENSMUSP00000000445.2, ENSMUST00000000445.2

Conserved Domains (3) summary

smart00520
Location:1 → 88

BASIC; Basic domain in HLH proteins of MYOD family

pfam12232
Location:143 → 214

Myf5; Myogenic determination factor 5

cd18937
Location:83 → 146

bHLH_TS_Myf5; basic helix-loop-helix (bHLH) domain found in myogenic factor 5 (Myf-5) and similar proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

107318769..107322787 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006513319.3 → XP_006513382.1 myogenic factor 5 isoform X1

See identical proteins and their annotated locations for XP_006513382.1

UniProtKB/Swiss-Prot

P24699, Q543W7

UniProtKB/TrEMBL

A2RSK4

Conserved Domains (3) summary

smart00520
Location:1 → 88

BASIC; Basic domain in HLH proteins of MYOD family

pfam12232
Location:143 → 214

Myf5; Myogenic determination factor 5

cd18937
Location:83 → 146

bHLH_TS_Myf5; basic helix-loop-helix (bHLH) domain found in myogenic factor 5 (Myf-5) and similar proteins