LEU2 3-isopropylmalate dehydrogenase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: LEU2
Official Full Name: 3-isopropylmalate dehydrogenase
Primary source: SGD:S000000523
Locus tag: YCL018W
See related: AllianceGenome:SGD:S000000523; FungiDB:YCL018W; VEuPathDB:YCL018W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables 3-isopropylmalate dehydrogenase activity. Involved in L-leucine biosynthetic process and glyoxylate cycle. Located in cytosol. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See LEU2 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 1

Sequence:: Chromosome: III; NC_001135.5 (91324..92418)

Chromosome III - NC_001135.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001135.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The ero1-1 leu2 strain does not grow in standard synthetic complete medium at 30 degrees C, a defect that can be remedied by increasing the L-leucine concentration in the medium or by transforming the ero1-1 leu2 strain with the LEU2 wild-type allele.
Title: Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3-isopropylmalate dehydrogenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3-isopropylmalate dehydrogenase activity	IDA Inferred from Direct Assay more info	PubMed
enables 3-isopropylmalate dehydrogenase activity	IEA Inferred from Electronic Annotation more info
enables 3-isopropylmalate dehydrogenase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables NAD binding	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in L-leucine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in L-leucine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in L-leucine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glyoxylate cycle	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: 3-isopropylmalate dehydrogenase

NP_009911.2

EC 1.1.1.85

Beta-isopropylmalate dehydrogenase (IMDH); catalyzes the third step in the leucine biosynthesis pathway; can additionally catalyze the conversion of beta-ethylmalate into alpha-ketovalerate

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001135.5 Reference assembly

Range

91324..92418

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178665.1 → NP_009911.2 TPA: 3-isopropylmalate dehydrogenase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009911.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQZ6, P04173

UniProtKB/TrEMBL

A6ZTG8, B5VET6, C7GQK2, C8Z469, G2WA04

Conserved Domains (1) summary

TIGR00169
Location:6 → 359

leuB; 3-isopropylmalate dehydrogenase