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    KAR9 Kar9p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 855859, updated on 9-Dec-2024

    Summary

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    Official Symbol
    KAR9
    Official Full Name
    Kar9p
    Primary source
    SGD:S000006190
    Locus tag
    YPL269W
    See related
    AllianceGenome:SGD:S000006190; FungiDB:YPL269W; VEuPathDB:YPL269W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Involved in establishment of spindle localization; mitotic spindle orientation checkpoint signaling; and nuclear migration along microtubule. Located in several cellular components, including kinetochore; mating projection tip; and microtubule cytoskeleton. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See KAR9 in Genome Data Viewer
    Location:
    chromosome: XVI
    Exon count:
    1
    Sequence:
    Chromosome: XVI; NC_001148.4 (33013..34947)

    Chromosome XVI - NC_001148.4Genomic Context describing neighboring genes Neighboring gene F1F0 ATP synthase subunit epsilon Neighboring gene ATP-binding cassette permease MDL2 Neighboring gene phosphatidylinositol phospholipase C Neighboring gene Acm1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001148.4

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Short Linear Sequence Motif LxxPTPh Targets Diverse Proteins to Growing Microtubule Ends. Kumar A, et al. Structure, 2017 Jun 6. PMID 28552577
    2. Purification, crystallization and preliminary crystallographic analysis of Kar9p from Saccharomyces cerevisiae. Hüls D, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2012 Oct 1. PMID 23027760, Free PMC Article
    3. Temporal control of contact site formation reveals a relationship between mitochondrial division and Num1-mediated mitochondrial tethering. Harper CS, et al. Mol Biol Cell, 2023 Oct 1. PMID 37585290, Free PMC Article
    4. Astral microtubule pivoting promotes their search for cortical anchor sites during mitosis in budding yeast. Baumgärtner S, et al. PLoS One, 2014. PMID 24721997, Free PMC Article
    5. Molecular linkage underlying microtubule orientation toward cortical sites in yeast. Korinek WS, et al. Science, 2000 Mar 24. PMID 10731146

    See all (131) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Activation of the DNA damage checkpoint perturbs asymmetric localization of Kar9 to spindle pole bodies in Saccharomyces cerevisiae.
      Title: Activation of the DNA damage checkpoint perturbs asymmetric localization of Kar9 to spindle pole bodies in Saccharomyces cerevisiae.
    2. Multivalency ensures persistence of a +TIP body at specialized microtubule ends.
      Title: Multivalency ensures persistence of a +TIP body at specialized microtubule ends.
    3. KAR9 has microtubule end-binding motif LxxPTPh (where x and h represent any and hydrophobic amino acids, respectively).
      Title: Short Linear Sequence Motif LxxPTPh Targets Diverse Proteins to Growing Microtubule Ends.
    4. One crystal of Kar9p showed diffraction to 3 A resolution
      Title: Purification, crystallization and preliminary crystallographic analysis of Kar9p from Saccharomyces cerevisiae.
    5. Kar9 is ubiquitylated and degraded by the proteasome after phosphorylation by Cdk1.
      Title: Ubiquitylation regulates interactions of astral microtubules with the cleavage apparatus.
    6. sumoylation regulates spindle positioning by restricting Kar9p to one spindle pole body.
      Title: The spindle positioning protein Kar9p interacts with the sumoylation machinery in Saccharomyces cerevisiae.
    7. Three independent mechanisms contribute to Kar9 asymmetry: Cdk1-dependent phosphorylation, sumoylation, and spindle-assembly checkpoint signaling.
      Title: Regulation of mitotic spindle asymmetry by SUMO and the spindle-assembly checkpoint in yeast.
    8. Kar9p-mediated MT delivery along actin cables does not involve Bud6p at the cell cortex
      Title: Differential contribution of Bud6p and Kar9p to microtubule capture and spindle orientation in S. cerevisiae.
    9. Overproduction of Bim1, but not Kar9, restores Kar9-dependent spindle positioning in the tub4-delta dsyl mutant, reduces cortical dwelling, and promotes Bim1-Kar9 interactions.
      Title: Gamma-tubulin is required for proper recruitment and assembly of Kar9-Bim1 complexes in budding yeast.
    10. The asymmetric localization of Kar9p to one spindle pole body and attached cytoplasmic microtubules is dependent on its interactions with microtubule-associated proteins and is regulated by the yeast Cdc28p
      Title: The cyclin-dependent kinase Cdc28p regulates multiple aspects of Kar9p function in yeast.
    Submit: New GeneRIF Correction See all GeneRIFs (11)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables molecular_function ND
    No biological Data available
    more info
    		  
    Process Evidence Code Pubs
    involved_in establishment of spindle localization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in establishment of spindle localization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in karyogamy IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mitotic spindle orientation checkpoint signaling IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mitotic spindle orientation checkpoint signaling IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in nuclear migration along microtubule IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in nuclear migration along microtubule IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cell cortex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cell cortex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasmic microtubule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoskeleton IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in kinetochore IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in mating projection tip IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in mating projection tip IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in microtubule IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in spindle pole body IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in spindle pole body IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    Kar9p
    NP_015054.1
    • Spindle positioning factor; orients astral microtubules, connecting them to actin cables at the cortex with Bim1p and Myo2, resulting in proper spindle positioning; targeted for StuBL-dependent degradation at kinetochores by Slx5p-Slx8p, ensuring chromosome transmission fidelity and correct spindle positioning; role in karyogamy; localizes to the shmoo tip, the growing bud-tip, the nucleus, the kinetochore, the spindle and microtubules; homolog of adenomatous polyposis coli

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001148.4 Reference assembly

      Range
      33013..34947
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001184083.1 → NP_015054.1  TPA: Kar9p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_015054.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6W3A1, P32526, Q08982
      UniProtKB/TrEMBL
      B3LKG4, B5VSR9, C7GSG9, C8ZIB0, N1NVN2
      Conserved Domains (1) summary
      pfam08580
      Location:84 → 406
      KAR9; Yeast cortical protein KAR9
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P32526.2

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