U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    Gad1 glutamate decarboxylase 1 [ Mus musculus (house mouse) ]

    Gene ID: 14415, updated on 3-Dec-2024

    Summary

    Official Symbol
    Gad1provided by MGI
    Official Full Name
    glutamate decarboxylase 1provided by MGI
    Primary source
    MGI:MGI:95632
    See related
    Ensembl:ENSMUSG00000070880 AllianceGenome:MGI:95632
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    EP10; Gad-1
    Summary
    The protein encoded by this gene is an enzyme that catalyzes the production of gamma-aminobutyric acid from L-glutamic acid. Alternative splicing of this gene results in three products, the predominant 67 kDa isoform, a smaller 25 kDa isoform, and an even smaller 49 aa isoform. The two shorter isoforms are not thought to retain catalytic activity. [provided by RefSeq, Apr 2023]
    Expression
    Biased expression in frontal lobe adult (RPKM 99.8), cerebellum adult (RPKM 45.5) and 4 other tissues See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See Gad1 in Genome Data Viewer
    Location:
    2 C2; 2 41.63 cM
    Exon count:
    19
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (70392109..70432358)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (70561303..70602014)

    Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_04762 Neighboring gene trans-acting transcription factor 5 Neighboring gene glutamate decarboxylase 1, opposite strand Neighboring gene glutamate rich 2 Neighboring gene STARR-seq mESC enhancer starr_04764 Neighboring gene predicted pseudogene 16510 Neighboring gene predicted gene, 46745

    Genomic regions, transcripts, and products

    Expression

    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Variation

    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (5) 
    • Targeted (15)  1 citation

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables glutamate binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables glutamate decarboxylase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables glutamate decarboxylase activity IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    enables glutamate decarboxylase activity ISO
    Inferred from Sequence Orthology
    more info
     
    enables identical protein binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables identical protein binding ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    enables protein-containing complex binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables pyridoxal phosphate binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables pyridoxal phosphate binding ISO
    Inferred from Sequence Orthology
    more info
     
    Component Evidence Code Pubs
    is_active_in GABA-ergic synapse IDA
    Inferred from Direct Assay
    more info
    PubMed 
    is_active_in GABA-ergic synapse IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    located_in axon IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in axon terminus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in cell cortex IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane TAS
    Traceable Author Statement
    more info
     
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in inhibitory synapse IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in mitochondrion HDA PubMed 
    located_in neuron projection terminus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in neuronal cell body ISO
    Inferred from Sequence Orthology
    more info
     
    is_active_in perinuclear region of cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    is_active_in presynapse IDA
    Inferred from Direct Assay
    more info
    PubMed 
    is_active_in presynapse IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    is_active_in presynaptic active zone IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in presynaptic active zone IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in presynaptic active zone ISO
    Inferred from Sequence Orthology
    more info
    PubMed 
    located_in synapse IDA
    Inferred from Direct Assay
    more info
    PubMed 

    General protein information

    Preferred Names
    glutamate decarboxylase 1
    Names
    67 kDa glutamic acid decarboxylase
    glutamic acid decarboxylase 1
    NP_001299829.1
    NP_001407028.1
    NP_001407029.1
    NP_001407030.1
    NP_001407031.1
    NP_001407032.1
    NP_001407033.1
    NP_001407034.1
    NP_001407035.1
    NP_032103.2

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001312900.2NP_001299829.1  glutamate decarboxylase 1 isoform GAD25

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) contains an alternate terminal exon in place of the last eleven exons compared to variant 1. The resulting isoform (GAD25) has a shorter and distinct C-terminus compared to isoform GAD67.
      Source sequence(s)
      AC097273
      Consensus CDS
      CCDS84535.1
      UniProtKB/TrEMBL
      D3Z1L8, D3Z5S3
      Related
      ENSMUSP00000119733.2, ENSMUST00000148210.8
      Conserved Domains (1) summary
      cl18945
      Location:143212
      AAT_I; Aspartate aminotransferase (AAT) superfamily (fold type I) of pyridoxal phosphate (PLP)-dependent enzymes. PLP combines with an alpha-amino acid to form a compound called a Schiff base or aldimine intermediate, which depending on the reaction, is the ...
    2. NM_001420099.1NP_001407028.1  glutamate decarboxylase 1 isoform GAD67

      Status: REVIEWED

      Source sequence(s)
      AC097273
      UniProtKB/Swiss-Prot
      O08685, P48318
      UniProtKB/TrEMBL
      Q548L6
    3. NM_001420100.1NP_001407029.1  glutamate decarboxylase 1 isoform GAD67

      Status: REVIEWED

      Source sequence(s)
      AC097273
      UniProtKB/Swiss-Prot
      O08685, P48318
      UniProtKB/TrEMBL
      Q548L6
    4. NM_001420101.1NP_001407030.1  glutamate decarboxylase 1 isoform GAD67

      Status: REVIEWED

      Source sequence(s)
      AC097273
      UniProtKB/Swiss-Prot
      O08685, P48318
      UniProtKB/TrEMBL
      Q548L6
    5. NM_001420102.1NP_001407031.1  glutamate decarboxylase 1 isoform GAD25

      Status: REVIEWED

      Source sequence(s)
      AC097273
      UniProtKB/TrEMBL
      D3Z1L8, D3Z5S3
    6. NM_001420103.1NP_001407032.1  glutamate decarboxylase 1 isoform GAD25

      Status: REVIEWED

      Source sequence(s)
      AC097273
      UniProtKB/TrEMBL
      D3Z1L8, D3Z5S3
      Related
      ENSMUSP00000119379.2, ENSMUST00000130998.8
    7. NM_001420104.1NP_001407033.1  glutamate decarboxylase 1 isoform GAD25

      Status: REVIEWED

      Source sequence(s)
      AC097273
      UniProtKB/TrEMBL
      D3Z1L8, D3Z5S3
      Related
      ENSMUSP00000116301.2, ENSMUST00000123330.3
    8. NM_001420105.1NP_001407034.1  glutamate decarboxylase 1 isoform c

      Status: REVIEWED

      Source sequence(s)
      AC097273
      UniProtKB/TrEMBL
      D3Z466
      Related
      ENSMUSP00000117521.2, ENSMUST00000130618.8
    9. NM_001420106.1NP_001407035.1  glutamate decarboxylase 1 isoform c

      Status: REVIEWED

      Source sequence(s)
      AC097273
      UniProtKB/TrEMBL
      D3Z466
    10. NM_008077.6NP_032103.2  glutamate decarboxylase 1 isoform GAD67

      See identical proteins and their annotated locations for NP_032103.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (GAD67).
      Source sequence(s)
      AC097273
      Consensus CDS
      CCDS16108.1
      UniProtKB/Swiss-Prot
      O08685, P48318
      UniProtKB/TrEMBL
      Q548L6
      Related
      ENSMUSP00000092539.5, ENSMUST00000094934.11
      Conserved Domains (1) summary
      pfam00282
      Location:143517
      Pyridoxal_deC; Pyridoxal-dependent decarboxylase conserved domain

    RNA

    1. NR_185134.1 RNA Sequence

      Status: REVIEWED

      Source sequence(s)
      AC097273
    2. NR_185135.1 RNA Sequence

      Status: REVIEWED

      Source sequence(s)
      AC097273

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000068.8 Reference GRCm39 C57BL/6J

      Range
      70392109..70432358
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    RNA

    1. XR_003950661.2 RNA Sequence

    2. XR_003950660.2 RNA Sequence