Myo5b myosin Vb [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Myo5bprovided by RGD
Official Full Name: myosin Vbprovided by RGD
Primary source: RGD:621347
See related: EnsemblRapid:ENSRNOG00000014104 AllianceGenome:RGD:621347
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Myr6
Summary: Enables ATP hydrolysis activity; ionotropic glutamate receptor binding activity; and small GTPase binding activity. Involved in several processes, including cellular response to glycine; endosomal transport; and positive regulation of neurogenesis. Located in several cellular components, including dendritic spine; neuronal cell body; and recycling endosome. Is active in several cellular components, including Schaffer collateral - CA1 synapse; postsynaptic actin cytoskeleton; and postsynaptic recycling endosome membrane. Human ortholog(s) of this gene implicated in microvillus inclusion disease and progressive familial intrahepatic cholestasis. Orthologous to human MYO5B (myosin VB). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Kidney (RPKM 192.5), Lung (RPKM 58.0) and 6 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 18q12.2

Exon count:: 45

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	18	NC_086036.1 (70313717..70613918)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	18	NC_051353.1 (68038759..68341568)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	18	NC_005117.4 (70426865..70729985)

Chromosome 18 - NC_086036.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Which bridges Myo5b and the membrane-bound Rab11a.
Title: Regulation of Myosin-5b by Rab11a and the Rab11 family interacting protein 2.
Rab10 interaction with myosin Vb (MYO5B) determines the formation of Rab10 carriers and is important for axon development.
Title: Myosin Vb controls biogenesis of post-Golgi Rab10 carriers during axon development.
Results indicate that Rab11a, Rab8a, and Myo5B function as part of a network to promote stretch-induced exocytosis.
Title: A Rab11a-Rab8a-Myo5B network promotes stretch-regulated exocytosis in bladder umbrella cells.
the efficient transport of NPC1L1 to the PM is dependent on the microfilament-associated myosin Vb.Rab11a.Rab11-FIP2 triple complex
Title: Requirement of myosin Vb.Rab11a.Rab11-FIP2 complex in cholesterol-regulated translocation of NPC1L1 to the cell surface.
These results support a model whereby AS160, Rab8A, and myosin Vb are required for insulin-induced GLUT4 translocation in muscle cells, potentially as part of a linear signaling cascade.
Title: Muscle cells engage Rab8A and myosin Vb in insulin-dependent GLUT4 translocation.
myosin Vb has a role in in glutamate receptor GluR1 trafficking
Title: Involvement of myosin Vb in glutamate receptor trafficking.
myosin Vb functions in traffic between peripheral and pericentrosomal compartments
Title: Chemical-genetic inhibition of a sensitized mutant myosin Vb demonstrates a role in peripheral-pericentriolar membrane traffic.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

d18rat10 (e-PCR)
- UniSTS:120231

BF403782 (e-PCR)
- UniSTS:246514

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables cytoskeletal motor activity	IEA Inferred from Electronic Annotation more info
enables ionotropic glutamate receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables microfilament motor activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	IEA Inferred from Electronic Annotation more info
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to glycine	IEP Inferred from Expression Pattern more info	PubMed
involved_in dendritic spine morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in endosomal transport	IEA Inferred from Electronic Annotation more info
involved_in endosomal transport	ISO Inferred from Sequence Orthology more info
involved_in endosomal transport	ISS Inferred from Sequence or Structural Similarity more info
involved_in modulation of chemical synaptic transmission	ISO Inferred from Sequence Orthology more info
involved_in neurotransmitter receptor transport, endosome to plasma membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in neurotransmitter receptor transport, endosome to plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of axon extension	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of exosomal secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein localization to plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of modification of postsynaptic structure	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of modification of postsynaptic structure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic vesicle recycling via endosome	IDA Inferred from Direct Assay more info	PubMed
involved_in synaptic vesicle recycling via endosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vesicle-mediated transport	ISO Inferred from Sequence Orthology more info
involved_in vesicle-mediated transport in synapse	IDA Inferred from Direct Assay more info	PubMed
involved_in vesicle-mediated transport in synapse	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in Schaffer collateral - CA1 synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in Schaffer collateral - CA1 synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in Schaffer collateral - CA1 synapse	ISO Inferred from Sequence Orthology more info
is_active_in actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in apical cortex	IEA Inferred from Electronic Annotation more info
located_in apical cortex	ISO Inferred from Sequence Orthology more info
located_in apical cortex	ISS Inferred from Sequence or Structural Similarity more info
located_in brush border	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in dendritic spine	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
part_of myosin complex	IEA Inferred from Electronic Annotation more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic recycling endosome membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic recycling endosome membrane	IMP Inferred from Mutant Phenotype more info	PubMed
located_in presynapse	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
located_in recycling endosome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: unconventional myosin-Vb

Names: Myosin of the dilute-myosin-V family; myosin 5B; myosin heavy chain myr 6

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_017083.2 → NP_058779.2 unconventional myosin-Vb

Status: VALIDATED

Source sequence(s)

JAXUCZ010000018

UniProtKB/TrEMBL

F1M3R4

Related

ENSRNOP00000019512.7, ENSRNOT00000019512.8

Conserved Domains (4) summary

smart00015
Location:863 → 883

IQ; Calmodulin-binding motif

COG1196
Location:901 → 1218

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd15477
Location:1472 → 1843

Myo5b_CBD; Cargo binding domain of myosin 5b

cd01380
Location:83 → 751

MYSc_Myo5; class V myosin, motor domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086036.1 Reference GRCr8

Range

70313717..70613918

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063277100.1 → XP_063133170.1 unconventional myosin-Vb isoform X8

UniProtKB/TrEMBL

A0A0G2K318

Related

ENSRNOP00000105443.1, ENSRNOT00000154755.1
XM_063277099.1 → XP_063133169.1 unconventional myosin-Vb isoform X5
XM_008772085.4 → XP_008770307.1 unconventional myosin-Vb isoform X1

UniProtKB/TrEMBL

F1M3R4

Conserved Domains (10) summary

smart00015
Location:871 → 891

IQ; Calmodulin-binding motif

smart00242
Location:72 → 770

MYSc; Myosin. Large ATPases

cd15477
Location:1480 → 1851

Myo5b_CBD; Cargo binding domain of myosin 5b

cd01380
Location:91 → 759

MYSc_Myo5; class V myosin, motor domain

pfam04871
Location:1155 → 1267

Uso1_p115_C; Uso1 / p115 like vesicle tethering protein, C terminal region

pfam07956
Location:910 → 1002

DUF1690; Protein of Unknown function (DUF1690)

pfam09311
Location:919 → 1179

Rab5-bind; Rabaptin-like protein

cd16269
Location:1385 → 1396

GBP_C; coiled coil [structural motif]

cl20817
Location:1340 → 1416

GBP_C; Guanylate-binding protein, C-terminal domain

cl23720
Location:979 → 1069

RILP-like; Rab interacting lysosomal protein-like 1 and 2 (Rilpl1 and Rilpl2)
XM_017600855.3 → XP_017456344.1 unconventional myosin-Vb isoform X6

UniProtKB/TrEMBL

A0A8I5ZST3, A0A8I6ACS1

Related

ENSRNOP00000081367.1, ENSRNOT00000094389.2
XM_063277098.1 → XP_063133168.1 unconventional myosin-Vb isoform X4
XM_039096575.2 → XP_038952503.1 unconventional myosin-Vb isoform X10

UniProtKB/TrEMBL

A0A8I6G4V2

Conserved Domains (4) summary

smart00015
Location:871 → 891

IQ; Calmodulin-binding motif

cd15477
Location:1434 → 1805

Myo5b_CBD; Cargo binding domain of myosin 5b

TIGR02168
Location:906 → 1244

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

cd01380
Location:91 → 759

MYSc_Myo5; class V myosin, motor domain
XM_063277102.1 → XP_063133172.1 unconventional myosin-Vb isoform X11
XM_017600852.3 → XP_017456341.1 unconventional myosin-Vb isoform X2

UniProtKB/TrEMBL

F1M3R4
XM_008772086.4 → XP_008770308.1 unconventional myosin-Vb isoform X7

UniProtKB/TrEMBL

A0A8I6ACS1

Conserved Domains (8) summary

smart00015
Location:871 → 891

IQ; Calmodulin-binding motif

smart00242
Location:72 → 770

MYSc; Myosin. Large ATPases

cd15477
Location:1453 → 1824

Myo5b_CBD; Cargo binding domain of myosin 5b

cd01380
Location:91 → 759

MYSc_Myo5; class V myosin, motor domain

pfam04871
Location:1154 → 1266

Uso1_p115_C; Uso1 / p115 like vesicle tethering protein, C terminal region

pfam07956
Location:910 → 1002

DUF1690; Protein of Unknown function (DUF1690)

pfam14915
Location:924 → 1216

CCDC144C; CCDC144C protein coiled-coil region

cl23720
Location:979 → 1069

RILP-like; Rab interacting lysosomal protein-like 1 and 2 (Rilpl1 and Rilpl2)
XM_063277101.1 → XP_063133171.1 unconventional myosin-Vb isoform X9
XM_063277103.1 → XP_063133173.1 unconventional myosin-Vb isoform X12
XM_063277104.1 → XP_063133174.1 unconventional myosin-Vb isoform X13
XM_063277105.1 → XP_063133175.1 unconventional myosin-Vb isoform X13
XM_017600854.3 → XP_017456343.1 unconventional myosin-Vb isoform X3

UniProtKB/TrEMBL

F1M3R4

Related

ENSRNOP00000069482.3, ENSRNOT00000085318.3
XM_006254908.5 → XP_006254970.1 unconventional myosin-Vb isoform X14

UniProtKB/TrEMBL

F1M3R4

Conserved Domains (7) summary

smart00015
Location:625 → 645

IQ; Calmodulin-binding motif

smart00242
Location:1 → 524

MYSc; Myosin. Large ATPases

cd15477
Location:1234 → 1605

Myo5b_CBD; Cargo binding domain of myosin 5b

cl22853
Location:1 → 513

Motor_domain; Myosin and Kinesin motor domain

pfam07956
Location:664 → 756

DUF1690; Protein of Unknown function (DUF1690)

pfam09311
Location:673 → 933

Rab5-bind; Rabaptin-like protein

cl23720
Location:733 → 823

RILP-like; Rab interacting lysosomal protein-like 1 and 2 (Rilpl1 and Rilpl2)
XM_039096576.2 → XP_038952504.1 unconventional myosin-Vb isoform X15

UniProtKB/TrEMBL

A6KRF7, A6KRF8

Conserved Domains (2) summary

COG5022
Location:5 → 376

COG5022; Myosin heavy chain [General function prediction only]

cd15477
Location:761 → 1132

Myo5b_CBD; Cargo binding domain of myosin 5b