sas-6 Spindle assembly abnormal protein 6 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: sas-6
Official Full Name: Spindle assembly abnormal protein 6
Primary source: WormBase:WBGene00012888
Locus tag: CELE_Y45F10D.9
See related: AllianceGenome:WB:WBGene00012888
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables identical protein binding activity and protein domain specific binding activity. Involved in centriole replication; protein localization; and regulation of cell cycle. Located in centriole and cytoplasm. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See sas-6 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 4

Sequence:: Chromosome: IV; NC_003282.8 (13786945..13788731, complement)

Chromosome IV - NC_003282.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003282.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A primary microcephaly-associated sas-6 mutation perturbs centrosome duplication, dendrite morphogenesis, and ciliogenesis in Caenorhabditis elegans.
Title: A primary microcephaly-associated sas-6 mutation perturbs centrosome duplication, dendrite morphogenesis, and ciliogenesis in Caenorhabditis elegans.
The PP2A-mediated dephosphorylation promotes centriolar targeting of SAS-5 and ensures SAS-6 delivery to the site of centriole assembly.
Title: PP2A phosphatase acts upon SAS-5 to ensure centriole formation in C. elegans embryos.
SAS-6 is a component that is required for daughter centriole formation in C. elegans. SAS-6 is a coiled-coil protein that is recruited to centrioles at the onset of the centrosome duplication cycle.
Title: SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells.
Direct interactions of the SAS-6 coiled coil with ZYG-1 and SAS-5 are required to recruit SAS-6 to the mother centriole.
Title: Direct binding of SAS-6 to ZYG-1 recruits SAS-6 to the mother centriole for cartwheel assembly.
It was demonstrated that the short SAS-5 C-terminal domain (residues 390-404) specifically binds to a narrow central region (residues 275-288) of the SAS-6 coiled coil.
Title: SAS-6 coiled-coil structure and interaction with SAS-5 suggest a regulatory mechanism in C. elegans centriole assembly.
Study demonstrates that the kinase ZYG-1 phosphorylates the coiled-coil protein SAS-6 at serine 123 in vitro; this phosphorylation event is crucial for centriole formation in vivo.
Title: Phosphorylation of SAS-6 by ZYG-1 is critical for centriole formation in C. elegans embryos.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Y45F10D.9 (e-PCR)
- UniSTS:306554

Y45F10D.7 (e-PCR)
- UniSTS:308181

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cell cycle	IEA Inferred from Electronic Annotation more info
involved_in centriole replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in centriole replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in centriole	IBA Inferred from Biological aspect of Ancestor more info
located_in centriole	IDA Inferred from Direct Assay more info	PubMed
located_in centriole	IEA Inferred from Electronic Annotation more info
is_active_in centrosome	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in intracellular membraneless organelle	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Spindle assembly abnormal protein 6

NP_502660.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003282.8 Reference assembly

Range

13786945..13788731 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_070259.6 → NP_502660.1 Spindle assembly abnormal protein 6 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_502660.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O62479

Conserved Domains (3) summary

pfam04880
Location:231 → 268

NUDE_C; NUDE protein, C-terminal conserved region

pfam16531
Location:52 → 108

SAS-6_N; Centriolar protein SAS N-terminal

cl23943
Location:190 → 259

PCRF; PCRF domain