him-6 RecQ-like DNA helicase blm-1 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: him-6
Official Full Name: RecQ-like DNA helicase blm-1
Primary source: WormBase:WBGene00001865
Locus tag: CELE_T04A11.6
See related: AllianceGenome:WB:WBGene00001865
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables 3'-5' DNA helicase activity and enzyme binding activity. Involved in several processes, including determination of adult lifespan; meiotic nuclear division; and resolution of DNA recombination intermediates. Located in chromosome and nucleus. Used to study Bloom syndrome. Human ortholog(s) of this gene implicated in Bloom syndrome. Orthologous to human BLM (BLM RecQ like helicase). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See him-6 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 13

Sequence:: Chromosome: IV; NC_003282.8 (12490791..12495298)

Chromosome IV - NC_003282.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003282.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

we found that Caenorhabditis elegans replication protein A, a single-stranded DNA binding protein, suppresses the reiterative behavior of HIM-6 and induces unidirectional, processive unwinding, possibly through a direct interaction between the proteins.
Title: Molecular characteristics of reiterative DNA unwinding by the Caenorhabditis elegans RecQ helicase.
Our results suggest that SMC-5/6 and HIM-6 can synergistically regulate recombination intermediate metabolism and suppress ectopic recombination by controlling chromosome architecture during meiosis.
Title: The SMC-5/6 Complex and the HIM-6 (BLM) Helicase Synergistically Promote Meiotic Recombination Intermediate Processing and Chromosome Maturation during Caenorhabditis elegans Meiosis.
HIM-6 has DNA-dependent ATPase activity HIM-6 and helicase activity that proceeds in the 3'-5' direction and needs at least five 3' overhanging nucleotides. HIM-6 is also able to unwind DNA structures including D-loops and Holliday junctions.
Title: Characterization of the Caenorhabditis elegans HIM-6/BLM helicase: unwinding recombination intermediates.
him-6 mutants are competent to load the MutSgamma complex at crossing over sites, to generate intermediates that enable meiosis progression, and to regulate crossing over. HIM-6 prevents formation of MutSgamma-independent interhomolog associations.
Title: DNA helicase HIM-6/BLM both promotes MutSγ-dependent crossovers and antagonizes MutSγ-independent interhomolog associations during caenorhabditis elegans meiosis.
Our results are consistent with the existence of at least two redundant meiotic resolvase activities: one requiring HIM-6 and XPF-1, and the other dependent on SLX-1 and MUS-81.
Title: Combinatorial regulation of meiotic holliday junction resolution in C. elegans by HIM-6 (BLM) helicase, SLX-4, and the SLX-1, MUS-81 and XPF-1 nucleases.
HIM-6 has a role in preventing DNA lesions
Title: Bloom syndrome ortholog HIM-6 maintains genomic stability in C. elegans.
Needed to maintain low levels of double-strand breaks in normally proliferating germ cells.
Title: Multiple genetic pathways involving the Caenorhabditis elegans Bloom's syndrome genes him-6, rad-51, and top-3 are needed to maintain genome stability in the germ line.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

T04A11.6 (e-PCR)
- UniSTS:310645

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables 3'-5' DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3'-5' DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent activity, acting on DNA	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
enables four-way junction helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables isomerase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA unwinding involved in DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromosome organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in chromosome segregation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in determination of adult lifespan	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via homologous recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in meiotic DNA double-strand break processing involved in reciprocal meiotic recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in meiotic cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in meiotic chromosome separation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in metabolic process	IEA Inferred from Electronic Annotation more info
involved_in mitotic cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleic acid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in reproductive process	IEA Inferred from Electronic Annotation more info
involved_in resolution of DNA recombination intermediates	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in resolution of meiotic recombination intermediates	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to X-ray	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in chromosome	IBA Inferred from Biological aspect of Ancestor more info
located_in chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: RecQ-like DNA helicase blm-1

NP_502390.2

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003282.8 Reference assembly

Range

12490791..12495298

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_069989.7 → NP_502390.2 RecQ-like DNA helicase blm-1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_502390.2

Status: REVIEWED

UniProtKB/Swiss-Prot

O18017, Q8MZK2

Conserved Domains (6) summary

smart00341
Location:807 → 888

HRDC; Helicase and RNase D C-terminal

COG0514
Location:231 → 881

RecQ; Superfamily II DNA helicase RecQ [Replication, recombination and repair]

cd00079
Location:443 → 572

HELICc; Helicase superfamily c-terminal domain; associated with DEXDc-, DEAD-, and DEAH-box proteins, yeast initiation factor 4A, Ski2p, and Hepatitis C virus NS3 helicases; this domain is found in a wide variety of helicases and helicase related proteins; may ...

pfam00270
Location:252 → 419

DEAD; DEAD/DEAH box helicase

pfam09382
Location:653 → 772

RQC; RQC domain

pfam16124
Location:577 → 643

RecQ_Zn_bind; RecQ zinc-binding